|
|
| (4 intermediate revisions not shown) |
| Line 30: |
Line 30: |
| | <div id="page-content"> | | <div id="page-content"> |
| | | | |
| - | <div class="article" id="w02-14-05">
| |
| - | <div class="article-title">
| |
| - | <p><a name="week02-14-05">Week 2, 14.05.2013, Tuesday</a></p>
| |
| - | </div>
| |
| - | <div class="article-details">
| |
| - | <p>Posted on 10/06/2013</p>
| |
| - | </div>
| |
| - | <div class="article-text">
| |
| - | <p>
| |
| - | <b>1) Starting with experiment Glycerol dehydrogenase </b><br/>
| |
| | | | |
| - | - Preparing overnight culture ''E. coli'' KRX <br/>
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| | | | |
| - | <div class="article" id="w02-15-05">
| |
| - | <div class="article-title">
| |
| - | <p><a name="week01-15-05">Week 2, 15.05.2013, Wednesday</a></p>
| |
| - | </div>
| |
| - | <div class="article-details">
| |
| - | <p>Posted on 10/06/2013</p>
| |
| - | </div>
| |
| - | <div class="article-text">
| |
| - | <p>
| |
| - | <b>1) Glycerol dehydrogenase </b><br />
| |
| - | - Using overnight culture ''E. coli'' KRX <br />
| |
| - | - Genome Isolation ''E. coli'' KRX using innuPrep Plasmid MiniKit ''JenaAnalytik'' <br />
| |
| - | - Using ''E. coli'' KRX Genome DNA for PCR <br />
| |
| - | - PCR-program: <br />
| |
| | | | |
| - | <!-- [[Image:Bielefeld-Labjournal-22-05-2013-Tab1.jpg|100x150px|thumb|left|'''Molecular Biotechnology, Master''']]
| |
| - | <img align="right" width="500px" height="400" id="labj001" src="https://static.igem.org/mediawiki/2013/b/ba/Bielefeld-Labjournal-22-05-2013-Tab1.jpg"> -->
| |
| - | <br /><br />
| |
| | | | |
| - |
| |
| - | Table PCR-program <br />
| |
| - | - PCR products are used for agarose gel electrophoresis <br />
| |
| - | Table gel-program <br />
| |
| - | - Agarose gel electrophoresis failed, no bands found <br />
| |
| - | </p>
| |
| - | </div><!-- /article-text -->
| |
| - | </div><!-- /article -->
| |
| - |
| |
| - | <div class="article" id="w02-16-05">
| |
| - | <div class="article-title">
| |
| - | <p><a name="week02-16-05">Week 2, 16.05.2013, Thursday</a></p>
| |
| - | </div>
| |
| - | <div class="article-details">
| |
| - | <p>Posted on 10/06/2013</p>
| |
| - | </div>
| |
| - | <div class="article-text">
| |
| - | <p>
| |
| - | <b>1) Glycerol dehydrogenase </b><br />
| |
| - | - Using'' E. coli'' KRX Genome DNA for PCR <br />
| |
| - | - PCR-program: <br />
| |
| - | Table PCR-components <br />
| |
| - | Table PCR-program <br />
| |
| - | - PCR products are used for agarose gel electrophoresis <br />
| |
| - | Table gel-program <br />
| |
| - | - Agarose gel electrophoresis worked, see image. <br />
| |
| - | Picture Agarosegel glda_1 <br />
| |
| - | - Bands are at expected size of 1050 bp <br />
| |
| - | </p>
| |
| - | </div><!-- /article-text -->
| |
| - | </div><!-- /article -->
| |
| - |
| |
| - |
| |
| - | <div class="article" id="w02-17-05">
| |
| - | <div class="article-title">
| |
| - | <p><a name="week02-17-05">Week 2, 17.05.2013, Friday</a></p>
| |
| - | </div>
| |
| - | <div class="article-details">
| |
| - | <p>Posted on 10/06/2013</p>
| |
| - | </div>
| |
| - |
| |
| - | <div class="article-text">
| |
| - | <p>
| |
| - |
| |
| - | <b> 1) Glycerol dehydrogenase </b> <br />
| |
| - |
| |
| - | - Repeating agarose gel electrophoresis form 23.05.2013 with more sample volume <br />
| |
| - | - Isolation of bands at 1050 bp using innuPrep Gel Extraction Kit ''JenaAnalytik'' <br />
| |
| - | - Measurement of DNA-concentration using nanodrop <br />
| |
| - | <br />
| |
| - | </p>
| |
| - | </div><!-- /article-text -->
| |
| - | </div><!-- /article -->
| |
| | </div><!-- /page-content --> | | </div><!-- /page-content --> |
| | <!-- </div> /page-middle --> | | <!-- </div> /page-middle --> |
| | | | |
| | </html> | | </html> |
"
