Team:BYU Provo/Notebook/SmallPhage/Springexp/Period4/Dailylog

From 2013.igem.org

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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: <u> '''Small Phage''' </u> </font>
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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<font size="4"> '''6/17/13''' </font>
<font size="4"> '''6/17/13''' </font>
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- Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
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- Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]]
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<font size="4"> '''6/18/13''' </font>
<font size="4"> '''6/18/13''' </font>
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- JADE, HOW MUCH OVERNIGHT DID YOU START?
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- Started approximately 50mL of BL21 liquid culture overnight.
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<font size="4"> '''6/19/13''' </font>
<font size="4"> '''6/19/13''' </font>
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- We performed Large Plaque Confirmation 1 as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
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- We performed Large Plaque Confirmation 1 as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]]
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<font size="4"> '''5/30/13''' </font>
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<font size="4"> '''6/23/13''' </font>
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- Plates from yesterday are taken out of incubation at around 4:00pm
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- Started 40mL of BL21 liquid culture overnight.
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<font size="4"> '''5/31/13''' </font>
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<font size="4"> '''6/24/13''' </font>
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- Discussed results for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]] -> will need to try this one more time with minor adjustment to experimental design: plate -4 dilution using x6 and x8 top agar.
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- Made more x8 top agar.
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- Because the large plaque confirmation 1 had contamination from overnight (the LB was contaminated the next day), we redid the confirmation procedure: Large Plaque Confirmation 2 in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Second Protocol]].
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- Discussed plans for next week.
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- Made new LB and x6 top agar.
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<font size="4"> '''6/2/13''' </font>
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<font size="4"> '''6/26/13''' </font>
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- Made about 20ml of BL21 overnight
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- Made about 50ml of BL21 liquid culture overnight
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<font size="4"> '''6/3/13''' </font>
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<font size="4"> '''6/27/13''' </font>
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- Made new LB plates
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- Started the mutagenesis procedure for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]]
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- Plated -4 titer on x6 and x8 plates for [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]
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<font size="4"> '''6/5/13''' </font>
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<font size="4"> '''6/28/13''' </font>
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- Discussed plans for the selection process of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]] and calculated the needed volume of agar, overnight, and phage
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- Performed first round of titer for [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.27_Mutagen_Concentration_Test_-_Third_Protocol|6.27 Mutagen Concentration Test - Third Protocol]]. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.
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- Made 500mL x8 agar
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- Performed dilution series to generate enough 200ug -4 phage stock for selection.
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<font size="4"> '''6/6/13''' </font>
 
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- Started approximately 50mL of BL21 liquid culture overnight.
 
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<font size="4"> '''6/7/13''' </font>
 
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- Performed the selection process of  [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]. Specifically, we plated 45 plates of 200ug mutagen, -4 phage dilution using x8 agar. 5 plates of 0ug, -3 phage dilution were also done a control.
 
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<font size="4"> '''6/9/13''' </font>
 
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- Started 21mL of BL21 overnight.
 
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<font size="4"> '''6/10/13''' </font>
 
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- Made 1250mL of x8 top agar
 
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- Attempted to amplify phage from larger plaques in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment|5.20 Mutagen Concentration Experiment]]
 
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<font size="4"> '''6/11/13''' </font>
 
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- Started approximately 70mL of BL21 liquid culture overnight
 
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<font size="4"> '''6/12/13''' </font>
 
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- Perfected protocol for applying mutagen to phage
 
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- Started [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
 
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<font size="4"> '''6/13/13''' </font>
 
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- Started approximately 30mL of BL21 liquid culture overnight.
 
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<font size="4"> '''6/14/13''' </font>
 
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- Titered the mutagenesis product from Wednesday as part of [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/6.12 Mutagen Concentration Test - Perfected Protocol|6.12 Mutagen Concentration Test - Perfected Protocol]]
 
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<font size="4"> '''6/16/13''' </font>
 
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- Started approximately 100mL of BL21 liquid culture overnight.
 
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Latest revision as of 15:31, 9 September 2013


Small Phage May - June Notebook: June 17 - June 30 Daily Log



Small Phage
March-April
May-June
July-August
September-October


6/17/13

- Plated 5 controls and 50 selection plates (500ug at -2) as part of selection 1 in 6.12 Mutagen Concentration Test - Second Protocol


6/18/13

- Started approximately 50mL of BL21 liquid culture overnight.


6/19/13

- We performed Large Plaque Confirmation 1 as part of 6.12 Mutagen Concentration Test - Second Protocol


6/23/13

- Started 40mL of BL21 liquid culture overnight.


6/24/13

- Made more x8 top agar. - Because the large plaque confirmation 1 had contamination from overnight (the LB was contaminated the next day), we redid the confirmation procedure: Large Plaque Confirmation 2 in 6.12 Mutagen Concentration Test - Second Protocol.


6/26/13

- Made about 50ml of BL21 liquid culture overnight


6/27/13

- Started the mutagenesis procedure for 6.27 Mutagen Concentration Test - Third Protocol


6/28/13

- Performed first round of titer for 6.27 Mutagen Concentration Test - Third Protocol. This includes 0, -2, -4, -6, and -8 for 0ug, 100ug, 200ug, and 500ug mutagen concentration.


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