Latest revision as of 20:59, 16 September 2013

11 June 2013

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helloworld-project

Every PCR tube contains

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-== main purposes today ==
+{{:Team:DTU-Denmark/Templates/StartPage|11 June 2013}}
+Navigate to the [[Team:DTU-Denmark/Notebook/10_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_June_2013|Next]] Entry
+=208 lab=
+<hr/>
+== Main purposes today ==
+<hr/>
 helloworld-project
 *diluting primers with 1.5 ml milli Q water each
 *diluting G-block with 20ul milli Q water
-==who were in the lab==
+==Who was in the lab==
+<hr/>
 ==Procedure==
+<hr/>
+[[Team:DTU-Denmark/Methods/PCR|PCR]]
-== Conclusion from today ==
+*7 rxns in duplicates
+*mastermix for 15 rxns
+{|
+! !!per rxn!!master
+|-
+|5x HF buffer||10ul||150ul
+|-
+|MgCl_2||0.5ul||7.5ul
+|-
+|dNTPs||5ul||75ul
+|-
+|Phusion polymerase||0.5ul||7.5ul
+|-
+|Milli Q water||25ul||375ul
+|-
+|master mix total || ||615 ul
+|}
+Every PCR tube contains
+{|
+|-
+|mastermix||41ul
+|-
+|FW primer||3ul
+|-
+|RV primer||3ul
+|-
+|DNA template||1ul
+|-
+|PCR tube total || ||50ul
+|}
+Navigate to the [[Team:DTU-Denmark/Notebook/10_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/12_June_2013|Next]] Entry
+{{:Team:DTU-Denmark/Templates/EndPage}}