Team:Hong Kong HKUST/module3backup
From 2013.igem.org
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<h3>Overview</h3> | <h3>Overview</h3> | ||
In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria. | In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria. | ||
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- | In our module, we will construct the MLS BioBrick | + | In our module, we will construct the MLS BioBrick <a href="http://parts.igem.org/Part:BBa_K1119001">K1119001</a>, and characterize it quantitatively[link]. |
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In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The ACE proteins should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). | In our project, we introduced bacterial enzymes to mammalian cell to modify metabolic pathway. However, unlike bacteria, citric acid cycle in mammalian cells is compartmentalized in mitochondria. The ACE proteins should be targeted to mitochondria for their functionality. To do so, we fused ACE enzymes with Mitochondrial Leader Sequence (MLS). | ||
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<h3>Reference</h3> | <h3>Reference</h3> | ||
Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2010). Essential cell biology. (3rd ed., p. 505). UK: Garland Science. | Alberts, B., Bray, D., Hopkin, K., Johnson, A., Lewis, J., Raff, M., Roberts, K., & Walter, P. (2010). Essential cell biology. (3rd ed., p. 505). UK: Garland Science. |
Latest revision as of 15:13, 27 September 2013
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Protein Trafficking
- Overview
- Mechanism of MLS
- Linkage to Project
- Reference
-
Modules
- FA Quantification & Cell Viability
- FA Sensing Mechanism
- Protein Trafficking
- Glyoxylate Shunt
Protein Trafficking
Overview
In nature, eukaryotic cell mitochondria usually have their protein encoded by gene in nucleus and produced from cytosol. In this process, MLS will act as signal peptide to target the protein into mitochondria.In our module, we will construct the MLS BioBrick K1119001, and characterize it quantitatively[link].