Team:OUC-China/Results

From 2013.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 3: Line 3:
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
-
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<link href="http://oucast.com/igem/css/bootstrap.css" rel="stylesheet" media="screen">
+
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
-
<link href="http://oucast.com/igem/css/bootstrap-responsive.css" rel="stylesheet" media="screen">
+
-
<link href="http://oucast.com/igem/css/docs.css" rel="stylesheet">
+
-
 
+
<style type="text/css">
<style type="text/css">
-
 
+
/*the igem Global setting*/
-
 
+
* {
 +
  margin: 0;
 +
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
 +
#menubar{
 +
  display: none;
 +
}
 +
body{
 +
  color: #34495e;
 +
  padding-top: 80px;
 +
}
 +
#bodyContent h1, #bodyContent h2{
 +
  margin-bottom:0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
  padding-top: 0;
 +
  padding-bottom: 0;
 +
  color: #34495e;
 +
}
 +
#globalWrapper {
 +
  font-size: 100%;
 +
}
#top-section{
#top-section{
   height:0px;
   height:0px;
Line 17: Line 39:
     margin:0 auto;
     margin:0 auto;
     padding:0 0 0 0;
     padding:0 0 0 0;
-
     background-color:#e5e5e5;
+
     background-color:transparent;
     overflow:hide;
     overflow:hide;
-
 
}
}
#p-logo{display:none;}
#p-logo{display:none;}
Line 34: Line 55:
#content
#content
{
{
-
border:none;
+
  border:none;
-
height: 100%;
+
  height: 100%;
-
margin:0;
+
  margin:0;
-
padding:0;
+
  padding:0;
-
width:100%;
+
  width:100%;
-
position: absolute;
+
  position: absolute;
-
background-color: #e5e5e5;
+
  background-color: #FFFFFF;
 +
  color: #34495e;
}
}
-
 
+
a:visited{
-
{
+
  color: #fff;
-
margin:0;
+
-
padding:0;
+
}
}
-
 
+
/*the nav settings
-
 
+
----------------------------------------------- */
-
#menubar {
+
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
-
    background-color:transparent;
+
  color: #1abc9c;
-
    position: relative;
+
  background-color: transparent;
-
    float:left;
+
-
    white-space: nowrap;
+
-
    top:-6px;
+
-
    width: 490px;
+
-
    z-index: 5000;
+
-
    font-family: sans-serif;
+
-
    font-size: 50%;
+
-
    line-height: 1em;
+
-
    z-index:99;
+
}
}
-
li {font-weight:normal;
+
.navbar-inverse .navbar-nav>li>a{
-
    font-size:12px;
+
  color: #fff;
 +
}
 +
.navbar-inverse .navbar-nav>li>a:hover{
 +
  color: #1abc9c;
 +
  background-color:transparent;
 +
}
 +
.navbar-inverse .navbar-brand{
 +
          color: #fff;
 +
          padding: 0;
 +
}
 +
.navbar-inverse .navbar-brand img{
 +
  height: 50px;
 +
  padding-left: 90px;
 +
}
 +
.navbar-inverse .navbar-brand p{
 +
  padding:12px 30px;
 +
  margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
  color: #1abc9c;
 +
}
 +
.navbar-inverse{
 +
  border-color: transparent;
 +
  background: #34495e;
 +
  font-weight: bold;
 +
  filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
 +
}
 +
.dropdown-menu{
 +
  background-color:#34495e;
 +
  border:none;
 +
  -webkit-box-shadow:none;
 +
  box-shadow: none;
 +
}
 +
.dropdown-menu>li>a{
 +
  color: #fff;
 +
  font-weight: bold;
 +
}
 +
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
 +
  color:#1abc9c;
 +
  background-color: transparent;
}
}
-
body {
+
/*add dropdown-submenu to btV3*/
-
      padding-bottom: 40px;
+
.dropdown-submenu{position:relative;}
-
      color: #5a5a5a;
+
.dropdown-submenu>.dropdown-menu{top:0;left:100%;margin-top:-6px;margin-left:-1px;-webkit-border-radius:0 6px 6px 6px;-moz-border-radius:0 6px 6px 6px;border-radius:0 6px 6px 6px;}
-
  background: #e5e5e5 url(bootstrap/img/moon.png);
+
.dropdown-submenu:hover>.dropdown-menu{display:block;}
-
  overflow-x: hidden;
+
.dropdown-submenu>a:after{display:block;content:" ";float:right;width:0;height:0;border-color:transparent;border-style:solid;border-width:5px 0 5px 5px;border-left-color:#cccccc;margin-top:5px;margin-right:-10px;}
-
  -webkit-user-select: none;
+
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
-
  -webkit-tap-highlight-color: rgba(0,0,0,0);
+
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
-
    }
+
-
h1 {color:#0099FF}
+
 +
/*footer*/
 +
footer{
 +
  margin-top: 100px;
 +
  background-color: #eceff1;
 +
  color: #bdc1c5;
 +
  font-size: 17px;
 +
  padding: 0;
}
}
-
 
+
.footer-banner{
-
a:not(.btn) {
+
  background-color: #34495e;
-
color: #00699E;
+
  color: #58F8D9;
-
-webkit-transition: 300ms;
+
  margin-left: 42px;
-
-moz-transition: 300ms;
+
  min-height: 286px;
-
transition: 300ms;
+
  padding: 0 30px 30px;
}
}
-
a:hover:not(.btn),
+
.footer-banner ul{
-
a:active:not(.btn) {
+
  list-style-type: none;
-
color: #9E0000;
+
-
text-decoration: none;
+
-
-webkit-transform: translateY(-8px);
+
-
-moz-transform: translateY(-8px);
+
-
transform: translateY(-8px);
+
}
}
-
 
+
.footer-banner ul li{
-
hr.soften {
+
  border-bottom:1px solid rgba(57, 107, 97, 0.53);
-
height: 1px;
+
  line-height: 19px;
-
margin: 30px 0;
+
  padding: 6px 0;
-
background-image: -webkit-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:   -moz-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:    -ms-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:      -o-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
border: 0;
+
}
}
-
 
+
.footer-banner a{
-
.nav a {
+
  color: #58F8D9;
-
-webkit-transition: 500ms;
+
}
-
-moz-transition: 500ms;
+
.footer-banner a:hover{
-
transition: 500ms;
+
  text-decoration: none;
 +
}
 +
.footer-title{
 +
  margin: 0 0 22px;
 +
  padding-top: 21px;
 +
  color: #1abc9c;
 +
  font-weight: bold;
 +
}
 +
.footerleft a{
 +
  color: #bdc1c5;
 +
}
 +
.footerleft a:visited {
 +
  color: #bdc1c5;
}
}
 +
/*slide nav*/
 +
.bs-docs-sidebar{
 +
  margin-bottom: 30px;
 +
  padding-top: 10px;
 +
  padding-bottom: 10px;
 +
}
 +
.bs-docs-sidebar a{
 +
  color: #34495e;
 +
}
 +
.bs-docs-sidebar a:hover{
 +
  color: #16A085;
 +
}
</style>
</style>
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
  <meta name="description" content="">
  <meta name="description" content="">
  <meta name="author" content="">
  <meta name="author" content="">
-
<title>Microfluidics</title>
+
<title>Overview</title>
</head>
</head>
-
 
<body>
<body>
-
<div class="navbar navbar-inverse navbar-fixed-top">
+
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
-
      <div class="navbar-inner">
+
  <div class="navbar-header">
-
        <div class="container">
+
    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
-
<div class="row"><div class="span3"><a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2013/6/6a/Ouc-igem1.jpg" alt="logo" style="width:70px;height:50px;margin-top:0px;padding:0 0 0 0;" class="brand png"></a></div>
+
      <span class="sr-only">The navigation</span>
-
          <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
        </button>
-
          </button>
+
    <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
-
            <div class="nav-collapse collapse">
+
      <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
-
                  <ul class="nav pull-right">
+
    </a>
-
  <li class="active"><a href="https://2013.igem.org/Team:OUC-China"><font size="4.5px">Home</font></a></li>
+
    <a class="navbar-brand" href="https://2013.igem.org/Team:OUC-China">
-
              <li class="dropdown">
+
      <p>OUC-China IGEM 2013</p>
-
                <a class="dropdown-toggle" id="drop4" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Project</font><b class="caret"></b>
+
    </a>
-
</a>
+
  </div>
-
                <ul id="menu1" class="dropdown-menu" role="menu" aria-labelledby="drop4">
+
    <div class="collapse navbar-collapse navbar-ex1-collapse">
-
<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a>
+
      <ul class="nav navbar-nav pull-right">
-
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a>
+
      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
-
                  <li class="dropdown-submenu">
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
                  <a tabindex="-1" href="#">Intercellular compartment</a>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
-
  <ul class="dropdown-menu">
+
              <ul class="dropdown-menu">
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
+
                    <li class="dropdown-submenu">
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
+
                      <a tabindex="-1" href="#">Intracellular compartment</a>
-
 
+
                <ul class="dropdown-menu">
-
  </ul>
+
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
-
  </li>
+
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
 +
                </ul>
 +
            </li>
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
-
  <ul class="dropdown-menu">
+
          <ul class="dropdown-menu">
-
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instruction">Instruction</a></li>
+
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
-
 
+
            </ul>
-
 
+
          </li>
-
  </ul>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
-
  </li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
+
-
                  <li role="presentation" class="divider"></li>
+
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
+
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
              <li class="dropdown">
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Modeling</font><b class="caret"></b></a>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
-
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                 <ul class="dropdown-menu">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
-
                  <li role="presentation" class="divider"></li>
 
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
              <li class="dropdown">
+
            <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
-
                <a  href="https://2013.igem.org/Team:OUC-China/Human Practice"><font size="4.5px">Human Practice</font></a>
+
      <li class="dropdown">
-
               
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
-
              </li>
+
              <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
-
<li class="dropdown">
+
-
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Team</font><b class="caret"></b></a>
+
-
              <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
-
  </ul>
+
        </ul>
-
              </li>
+
            </li>
-
  <li class="dropdown">
+
      <li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
-
                <a  href="https://2013.igem.org/Team:OUC-China/Safety"><font size="4.5px">Safety</font></a>
+
      <li class="dropdown">
-
               
+
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
-
              </li>
+
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
-
  <li class="dropdown">
+
-
                 <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Notebook</font><b class="caret"></b></a>
+
-
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab note">Lab note</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab note">Lab note</a></li>
-
                  <li role="presentation" class="divider"></li>
 
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
  </ul>
+
    </ul>
-
</div>
+
  </div>
-
          </div>
+
</div>  
-
        </div>
+
-
      </div>
+
-
    </div>
+
-
<br><br>
+
-
 
+
<div class="container">
<div class="container">
-
 
<div class="row">
<div class="row">
-
       <div class="span3 bs-docs-sidebar">
+
       <div class="col-md-3 bs-docs-sidebar">
-
        <ul class="nav nav-list bs-docs-sidenav">
+
      <ul class="nav nav-list bs-docs-sidenav">
           <li><a href="https://2013.igem.org/Team:OUC-China/Review"><i class="icon-chevron-right"></i>Review</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/Review"><i class="icon-chevron-right"></i>Review</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/Design"><i class="icon-chevron-right"></i>Design</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/Design"><i class="icon-chevron-right"></i>Design</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/Microfluidics"><i class="icon-chevron-right"></i>Microfluidics</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/Microfluidics"><i class="icon-chevron-right"></i>Microfluidics</a></li>
-
  <li><a href="#Results"><i class="icon-chevron-right"></i>Results</a></li>
+
      <li><a href="#Results"><i class="icon-chevron-right"></i>Results</a></li>
            
            
         </ul>
         </ul>
       </div>
       </div>
-
 
+
       <div class="col-md-9">
-
       <div class="span9">
+
  <section id="Overview">
-
+
           <p><img src="https://static.igem.org/mediawiki/2013/8/8b/Ouc-result1.jpg" width="400"/><br />Figure. 1 The laser confocal microscopy result of JM109 with no plasmid. There is no GFP gathering in view.<br /><img src="https://static.igem.org/mediawiki/2013/2/26/Ouc-result5.jpg" width="400"/><br />Figure. 2 The laser confocal microscopy results of JM109 with MamC::GFP expression plasmid(the left) and the JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster(the right).<br /><br />As we see there are obvious GFP signals generally equally distributed in JM109 with MamC::GFP expression plasmid, only a few bacteria contain the GFP gatherings at both ends of cells. And there no GFP signals in middle of the cell. There are GFP gatherings at both ends of cells. Compared with the JM109 with MamC::GFP expression plasmid, the figure(on the right) of JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster shows that there are clear MamC::GFP fluorescence gatherings at both ends and in middle area of the cell, which is different from the results of the other two samples. Additionally, we observed that the shape of the strain with both plasmids has changed. It has become much longer than normal <i>E.coli</i>, as the figure you can see(on the right). And the length of strain with both plasmids is almost three times longer than normal <i>E.coli.</i><br /> <br />We cannot be completely certain that the protein is actually attached to the membrane as we assumed, because the mechanism of the membrane formation is still not studied thoroughly. We cannot do TEM for the engineered bacteria at the moment due to forces beyond our control. You are welcome to follow us in our future work!
-
 
+
     <br/></p>
-
  <section id="Results">
+
  </section>
-
 
+
-
  <div class="page-header">
+
-
            <h1>Results</h1>
+
-
           </div>
+
-
  <div class="row">
+
-
 
+
-
  <div class="span9">
+
-
    <div class="row">
+
-
     
+
-
      <div class="span9"><p style="font-weight:normal;"><font size="2px"><img src="https://static.igem.org/mediawiki/2013/2/26/Ouc-result5.jpg" height="300" width="400"/><br /><img src="https://static.igem.org/mediawiki/2013/2/26/Ouc-result5.jpg" height="300" width="400"/><br />Figure. 2 The laser confocal microscopy results of JM109 with MamC::GFP expression plasmid(the left) and the JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster(the right).<br /><br />As we see there are obvious GFP signals generally equally distributed in JM109 with MamC::GFP expression plasmid, only a few bacteria contain the GFP gatherings at both ends of cells. And there no GFP signals in middle of the cell. There are GFP gatherings at both ends of cells. Compared with the JM109 with MamC::GFP expression plasmid, the figure(on the right) of JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster shows that there are clear MamC::GFP fluorescence gatherings at both ends and in middle area of the cell, which is different from the results of the other two samples. Additionally, we observed that the shape of the strain with both plasmids has changed. It has become much longer than normal E.coli, as the figure you can see(on the right). And the length of strain with both plasmids is almost three times longer than normal <i>E.coli.</i></font></p></div>
+
-
 
+
-
  <br/>
+
-
  </div>
+
-
     </div>
+
-
  </div>
+
-
</div><br /><br /></br>
+
-
+
-
    </section>
+
    </div>
    </div>
  </div>
  </div>
  </div>
  </div>
-
 
+
<!--footer-->
-
 
+
<footer>
-
 
+
<div class="container">
 +
<div class="row">
 +
<div class="col-md-1"></div>
 +
<div class="col-md-6 footerleft">
 +
  <h3 class="footer-title" style="color:#bdc1c5;">About</h3>
 +
  <p>Thanks to:</p>
 +
  <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Bootstrap&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Flat-ui&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Unslider</p>
 +
  <p>Designed and built by <a href="http://copypeng.com">@PengYong</a> and <a href="http://www.renren.com/345094399/profile?ref=opensearch_normal#notice">@ZhenYuchen.</a></p>
 +
  <p>Code licensed under Apache License v2.0</p>
 +
</div>
 +
<div class="col-md-4">
 +
  <div class="footer-banner">
 +
    <h3 class="footer-title">Contact US</h3>
 +
    <ul>
 +
      <li>E-mail:<a href="mailto:oucigem@163.com"> oucigem@163.com</a></li>
 +
      <li><a href="http://oucast.com"> Official website</a></li>
 +
      <li><a href="http://www.renren.com/oast"> Find us on Renren</a></li>
 +
      <li><a href="http://weibo.com/u/2805858363"> Find us on Weibo</a></li>
 +
      <li><a href="https://www.google.com/maps?q=+36.062884,+120.335542&sll=36.1360706,120.3648376&sspn=0.36606958894334163,0.9386959091014536&t=m&z=16">Find us on Google Map</a></li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="col-md-1"></div>
 +
</div>
 +
</div>
 +
</footer>
<script src="http://code.jquery.com/jquery.js"></script>
<script src="http://code.jquery.com/jquery.js"></script>
-
<script src="http://oucast.com/igem/js/bootstrap.min.js"></script>
+
<script src="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
-
<script src="http://oucast.com/igem/js/ouc2.js"></script>
+
-
<script src="http://oucast.com/igem/js/application.js"></script>
+
-
<script>
+
-
 
+
-
      !function ($) {
+
-
        $(function(){
+
-
          // carousel demo
+
-
          $('#myCarousel').carousel()
+
-
        })
+
-
      }(window.jQuery)
+
-
    </script>
+
-
<script src="http://oucast.com/igem/js/holder.js"></script>
+
</body>
</body>
</html>
</html>

Latest revision as of 04:04, 28 September 2013

Overview


Figure. 1 The laser confocal microscopy result of JM109 with no plasmid. There is no GFP gathering in view.

Figure. 2 The laser confocal microscopy results of JM109 with MamC::GFP expression plasmid(the left) and the JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster(the right).

As we see there are obvious GFP signals generally equally distributed in JM109 with MamC::GFP expression plasmid, only a few bacteria contain the GFP gatherings at both ends of cells. And there no GFP signals in middle of the cell. There are GFP gatherings at both ends of cells. Compared with the JM109 with MamC::GFP expression plasmid, the figure(on the right) of JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster shows that there are clear MamC::GFP fluorescence gatherings at both ends and in middle area of the cell, which is different from the results of the other two samples. Additionally, we observed that the shape of the strain with both plasmids has changed. It has become much longer than normal E.coli, as the figure you can see(on the right). And the length of strain with both plasmids is almost three times longer than normal E.coli.

We cannot be completely certain that the protein is actually attached to the membrane as we assumed, because the mechanism of the membrane formation is still not studied thoroughly. We cannot do TEM for the engineered bacteria at the moment due to forces beyond our control. You are welcome to follow us in our future work!

Retrieved from "http://2013.igem.org/Team:OUC-China/Results"