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(Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% A) |
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Latest revision as of 17:07, 27 October 2013
Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% AmilCP (blue) producing colonies. Non-recycled columns produced ~ 50% AmilCP (blue) producing colonies and ~50% RFP producing colonies from the previous experiment.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 17:07, 27 October 2013 |  | 725×351 (89 KB) | Pk197 (Talk | contribs) | |
| 17:05, 27 October 2013 |  | 742×375 (93 KB) | Pk197 (Talk | contribs) | ||
| 17:00, 27 October 2013 |  | 763×401 (99 KB) | Pk197 (Talk | contribs) | ||
| 19:01, 27 September 2013 |  | 800×446 (115 KB) | Raafekie (Talk | contribs) | (Columns were used to purify RFP expressing plasmid. Columns were recycled as per the recycling protocol (left) or not (right) and then used to purify AmilCP expressing plasmid. The elutant was transformed and plated. Recycled columns resulted in 100% A) |
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