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| - | <html>
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| - | <head>
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| - | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.10.2/jquery.min.js">
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| - | </script>
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| - | body{background-color:#d5cfbf;width:100%;margin-bottom:20px;min-width:600px;max-width:2000px;font: x-small sans-serif;}
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| - | #menubar
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| - | #menubar ul li a
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| - | #menubar.right-menu
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| - | {background-color:#EEECEC;opacity:0.8;top:-4px;width:240px;height:23px;position:absolute;top:-20px;}
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| - | #p-logo
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| - | .row {
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| - | min-width: 768px;
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| - | .menu ul,li
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| - | ul.first>li>a:hover,ul.first>li>a:active
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| - | .menu
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| - | position:absolute;
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| - | left:0px;
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| - | top:141px;
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| - | width:100%;
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| - | #back-top {
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| - | position: fixed;
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| - | top: 10%;
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| - | right: 2%;
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| - | }
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| - | #back-top a {
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| - | width: 108px;
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| - | text-align: center;
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| - | font: 11px/100% Arial, Helvetica, sans-serif;
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| - |
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| - | #back-top span {
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| - | width: 65px;
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| - | height: 60px;
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| - | display: block;
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| - | margin-bottom: 7px;
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| - |
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| - | ul.side-nav, ul.side-nav1 {
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| - | display: block;
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| - | list-style: none;
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| - | margin: 0;
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| - | padding: 17px 0;font-family:"Trebuchet MS", Helvetica, sans-serif;
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| - | ul.side-nav h6 {
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| - | ul.side-nav1 h6 {
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| - |
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| - | .two, .row .two {
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| - | width: 16.66667%;
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| - | top:160px;
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| - | }
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| - | ul, ol {
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| - | font-size: 14px;
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| - | [class*="column"] + [class*="column"]:last-child {
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| - | float: right;
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| - | .ten, .row .ten {
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| - | width: 83.33333%;
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| - | top:192px;
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| - | z-index:-2;
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| - | .column, .columns {
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| - | float: left;
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| - | min-height: 1px;
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| - | padding: 0 15px;
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| - | position: relative;
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| - | }
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| - | .twelve, .row .twelve {
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| - | width: 100%;
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| - | }
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| - | h1, h2, h4, h5, h6 {
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| - | font-family:"Trebuchet MS", Helvetica, sans-serif;
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| - | font-weight: normal;
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| - | color: #000;
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| - | border-bottom: none;
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| - |
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| - |
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| - | h3{font-size:18px;font-family:"Trebuchet MS", Helvetica, sans-serif;}
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| - | h5{margin-left:15px;}
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| - |
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| - | }
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| - | div.team-bios-container div.row {
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| - | padding: 0px 0px 10px;
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| - | border-bottom: 1px solid #ddd;
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| - | }
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| - | .row .row {
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| - | width: auto;
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| - | max-width: none;
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| - | min-width: 0;
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| - | margin: 0 -15px;
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| - | }
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| - | media="screen, projection"
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| - | #bodyContent h1, #bodyContent h2 {
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| - | margin-bottom: .6em;
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| - | }
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| - | h1.centered, h2.centered, h3.centered, h4.centered, h5.centered, h6.centered {
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| - | text-align: center;
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| - | }
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| - | h2 {
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| - | font-size: 37px;
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| - | }
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| - | .nine, .row .nine {
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| - | width: 100%;font-family:"Trebuchet MS", Helvetica, sans-serif;
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| - | }
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| - | [class*="column"] + [class*="column"]:last-child {
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| - | float: right;
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| - | }
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| - | .three, .row .three {
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| - | width: 25%;
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| - | }
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| - | #construct{margin-left:30px;}
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| - | ul li ul, ul li ol {
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| - | margin-left: 20px;
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| - | margin-bottom: 0;}
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| - | </style>
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| - |
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| - | <body>
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| - | <p id="back-top" style=""display:none;>
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| - | <a href="#top"><span><img src="http://515alive.com/theme/img/up-arrow.png" style="width:90%;"><br><br>BACK TO TOP</span></a>
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| - | </p>
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| - | <a href=https://2013.igem.org/Team:Hong_Kong_HKUST><center><div id="kepala" style="height:121px;width:100%;"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png" style="height:121px;width:100%;align:middle;"></div></center></a>
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| - | <div id="cover"></div>
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| - | <div class="menu">
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| - | <ul class="first">
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST">Home</a></li>
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/team">Team</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/members">Members</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/advisors">Advisors</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/instructors">Instructors</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/attribution">Attribution</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/acknowledge">Acknowledgement</a></li>
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| - | </ul>
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| - | </li>
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project">Project</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/abstract">Abstract</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/modules">Modules Description</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/data">Data Page</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Parts">Parts</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/characterization">Characterization</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/results">Result</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/future">Future Work</a></li>
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| - | </ul>
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| - | </li>
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Wetlab">Wetlab</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/experiment">Experiments</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/notebook">Notebook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/protocols">Protocols</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/safety">Safety</a></li>
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| - |
| |
| - | </ul>
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| - | </li>
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/achievement">Achievement</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/medals">Medal Requirements</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/prizes">Prizes</a></li>
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| - | </ul>
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| - | </li>
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| - |
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp">Human Practice</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/cp">Country Profile</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/blog">Blog</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/interview">Interviews</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/article/genet">Article</a></li>
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| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/video">Videos</a></li>
| |
| - | <li><a href="https://2013.igem.org/Team:Hong_Kong_HKUST/hp/presentation">Presentations</a></li>
| |
| - | </ul>
| |
| - | </li>
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| - |
| |
| - | </ul>
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| - |
| |
| - | </div>
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| - | <div class="row">
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| - | <div class="two columns">
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| - | <br><br>
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| - | <ul class="side-nav">
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| - | <li>
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| - | <h6>Modules</h6>
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| - | </li>
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| - | <li class="divider"></li>
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| - | <li>
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| - | FA Quantification & Cell Viability
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| - | <ul><li>
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| - | <a href=#1>Cell Line</a>
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| - | </li>
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| - | <li>
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| - | <a href=#2>Cell Culture</a>
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| - | </li></ul>
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| - | </li>
| |
| - | <li>
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| - | <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module2">FA Sensing Mechanism</a>
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| - | </li>
| |
| - | <li>
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| - | <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module3">Protein Trafficking</a>
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| - | </li>
| |
| - | <li>
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| - | <a href="https://2013.igem.org/Team:Hong_Kong_HKUST/Project/module4">Glyoxylate Shunt</a>
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| - | </li>
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| - | </ul>
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| - | </div>
| |
| - | <div class="ten columns team-bios-container">
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| - | <div class="row" id="ugd-members">
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| - | <div class="twelve columns">
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| - | <br>
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| - | <h2 class="centered">Fatty Acid Quantification and Cell Viability</h2>
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| - | </div>
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| - | </div>
| |
| - | <div class="row">
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| - | <div class="nine columns"><p id="1"></p>
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| - | <h3>Cell Line</h3>
| |
| - | For our project we made use of two mammalian cell lines: HepG2 human hepatoma cells, and HEK293FT human embryonic kidney cells. HepG2 cells were used for characterizing inducible promoters and the glyoxylate systems. To take advantage of their higher transfection efficiency, the characterizations of mitochondria leader sequence and constitutive promoter were conducted using HEK293FT cells.
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| - | <br>
| |
| - | </div>
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| - | </div>
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| - | <div class="row">
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| - | <div class="nine columns"><p id="2"></p>
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| - | <h3>Cell Culture</h3>
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| - | HepG2 and HEK293FT cells were grown in DMEM supplemented with 10% heat-inactivated FBS, 50 μg/mL penicillin and 50 μg/mL streptomycin. The cells were incubated at 37℃ in a humidified atmosphere containing 5% <p>CO<sub>2</sub>. Cells were transfected in petri dishes and multi-well plates with Lipofectamine 2000 (Invitrogen; Carlsbard, CA) transfection reagent according to the manufacturer’s protocols. GFP signals were observed under fluorescent microscope or under confocal microscope, if necessary.
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| - | </div>
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| - | </div>
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| - |
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| - |
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| - | </div>
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| - | </body>
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| - | </html>
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