Team:Berkeley

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                <li id="TitleID"> <a id="TitleID" href="https://2013.igem.org/Team:Berkeley">Project Blue Genes</a>  
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<div class = "heading"><a name="Project Description">Project Description</a></div>
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Many of the major advances in synthetic biology involve the ability to use microorganisms to synthesize compounds that are difficult or costly to produce chemically. Our team hopes to exploit this familiar application of synthetic biology for the production of indigo in E. Coli.</p>
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Indigo, the dye used to make blue jeans around the world, is produced in quantities of tens of thousands of tons every year. The chemical process involves converting vast quantities of aniline into indigo. Because indigo is extremely insoluble in water, this product must be reduced to leuco-indigo, a white soluble substance, using sodium dithionite. Upon immersing cotton in leuco-indigo and exposing the material to air, indigo reforms and binds to the cloth. The waste from this process is often discarded in large amounts, with significant environmental impact. Moreover, the process to produce analine uses a variety of hazardous chemicals including sulfuric acid and nitric acid. Our team explored biological production of indigo, biological alternatives to the current dyeing process, and potential industrial application of these alternatives.
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                <p>The industrial dyeing process is ecologically unfriendly - indigo is made from an oil derivative (benzene) using harsh chemicals and extremely high temperatures. Furthermore, for indigo to actually adhere to fabric, a reducing agent is necessary to solubilize the dye, and this process releases acidified wastewaters into local environments. </p> <p>Research has shown that the leaves of indigo-producing plants contain two enzymes, a glucosyltransferase (GT) and a glucosidase (GLU), which together control indigo solubility by interconverting indigo with a soluble form called indican. We propose a biological dyeing method that harnesses this pathway in a bacterial chassis. </p><p> For the first time, indican has been produced using a recombinant GT as a result of our work. We prove indican irreversibly dyes fabric with the use of a GLU. Lastly, we have examined  ways to reduce our new system’s costs by optimizing indoxyl (an indican precursor) production and taking into account other innovative design choices.</p><p><b> Our work has created an entirely new way to dye denim with purely biological inputs which, with continued optimization, could compete economically with current industrial methods.</b>  
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</p><p> <a href="https://2013.igem.org/Team:Berkeley/Project/Introduction">Read on</a> to learn more about our nature-inspired system!</p>
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                <div class="heading"><a name="Sponsors">&nbsp;&nbsp;Acknowledgements</a>
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                <p>The UC Berkeley iGEM team would like to thank Autodesk, Agilent, Synthetic Biology Institute, Qualcomm, Dean A. Richard Newton Memorial Chair for their financial support, IDT for discounted oligos and Quintara Bio for discounted sequencing service.</p>
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The UC Berkeley iGEM team would like to thank Autodesk, Agilent, Synthetic Biology Institute, Qualcomm, Dean A. Richard Newton Memorial Chair for their financial support, IDT for discounted oligos and Quintara Bio for discounted sequencing service.
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Latest revision as of 03:51, 29 October 2013

Project Blue Genes
Project Description

The industrial dyeing process is ecologically unfriendly - indigo is made from an oil derivative (benzene) using harsh chemicals and extremely high temperatures. Furthermore, for indigo to actually adhere to fabric, a reducing agent is necessary to solubilize the dye, and this process releases acidified wastewaters into local environments.

Research has shown that the leaves of indigo-producing plants contain two enzymes, a glucosyltransferase (GT) and a glucosidase (GLU), which together control indigo solubility by interconverting indigo with a soluble form called indican. We propose a biological dyeing method that harnesses this pathway in a bacterial chassis.

For the first time, indican has been produced using a recombinant GT as a result of our work. We prove indican irreversibly dyes fabric with the use of a GLU. Lastly, we have examined ways to reduce our new system’s costs by optimizing indoxyl (an indican precursor) production and taking into account other innovative design choices.

Our work has created an entirely new way to dye denim with purely biological inputs which, with continued optimization, could compete economically with current industrial methods.

Read on to learn more about our nature-inspired system!

Follow us on

  Acknowledgements

The UC Berkeley iGEM team would like to thank Autodesk, Agilent, Synthetic Biology Institute, Qualcomm, Dean A. Richard Newton Memorial Chair for their financial support, IDT for discounted oligos and Quintara Bio for discounted sequencing service.

getting code to count
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