Team:Korea U Seoul/Notebook sub

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Latest revision as of 20:26, 27 September 2013

March

1. Introduction to iGEM
- About synthetic biology : As an automobile can be described as assembly of numerous parts, synthetic biology is a field of assembled techniques from division of biotechnology. One of benefits offered in this field is that we can use necessary parts with lack of knowledge to specifics of each technique.
- Look around 2012 iGEM teams' posters, wiki pages

2. Division of roles
1) Experiment : Minseob Yoo, Hyeyeong Choi, Junhong Jang, Hyunsong Kim, Jihee Park, Juyoung Ha
2) Human Practice : Hyunsong Kim, Jihee Park, Hyeyeong Choi, Minseob Yoo
3) Creative Challenger Program (CCP) : Juyoung Han, Minseob Yoo, Hyunsong Kim
4) Design : Minseob Yoo
5) Modeling : Dongha Kim, Minwhan Yoo, Jonghee Chun
6) Fundraising : Kyengwoo Jang, Dongha Kim, Junhong Jang, Jihee Park
7) Clerk : Hyunsong Kim
8) Photographer : Jihoon Jeong

3. Fix meeting dates
- Meeing will be on every Monday, 7:00 p.m, lecture room 121 Life Science Division West building

4. Studying iGEM
- All members choose one team's result from past iGEM and make a presentation about it.

1) Jihoon Jeong - 2012 Cornell
2) Jaeuk Han - 2012 Slovenia
3) Hyeyeong Choi - 2011 Hong Kong-CUHK
4) Jihee Park - 2011 Brown-Stanford
5) Juyoung Han - 2012 PENN
6) Kyengwoo Jang - 2012 Caltech
7) Junhong Jang - 2012 Kyoto
8) Jonghee Chun - 2012 Arizona state
9) Minseob Yoo - 2012 Calgary
10) Dongha Kim - 2012 Groningen
11) Minwhan Yoo - 2011 Washington
12) Hyunsong Kim - 2012 Carnegie Mellon

5. Brainstorming for ideas

6. Human Practice
- Ask information agency about financial support and writing a newspaper article
- Participating in Science fair at COEX

Hyeyeong Choi : Collaborate with Korea University club "Uhai" by making a presentation about Synthetic Biology at Africa
Jihee Park: Making video introducing Synthetic Biology for elementary, middle and high school students
Juyoung Han : Producing cartoon that expains Synthetic Biology
Minwhan Yoo : Web-page production for KUAS(Korea University Association for Synthetic biology) beyond iGEM wiki page
Dongha Kim : Making flash gamem Manufacturing circuit board
Jonghee Chun : Homepage making
Junhong Jang : Realization of biobrick by flash

April

1. Ideas
(1) Keywords
- We made bulletin board at our website that we can easily search ideas

Kyengwoo Jang : Cyanobacteria+Rhodopsin, Acetic acid
Junhong Jang : MSG
Hyunsong Kim : Shwanella+Nano Silver
Juyoung Han : Aluminum
Jihoon Jeong : Proteorhodopsin
Jihee Park : Endospore inner membrane
Hyeyeong Choi : Food waste odor, Nuclear waste breakdown

(2) Teamwork
-We made team to think systemically

Group 1 (Jihoon Jeong, Minseob Yoo) : CaCO3, AR system
Group 2 (Jihee Park, Hyeyeong Choi, Kyengwoo Jang, Minseob Yoo, Juyoung Han) : Nonylphenol, SPR sensor, Lignin β-keto adipate pathway
Group 3 (Junhong Jang, Kyengwoo Jang, Hyunsong Kim, Juyoung Han) : Bio-hoil
Group 4 (Jihoon Jeong, Jaeuk Han, Minseob Yoo, Dongha Kim) : Bio-wire

We consolidated group 3&4 to the topic of nano metal producing by microbes

2. Human Practice
- Vote for Best 3 Human Practice from last year : Cornell, Calgary, LMU-Munich

May

1. Decide topic
- Minseob Yoo
: conchiolin is major protein of pearl
There are two types of crystal structures of CaCO3, calcite and aragonite
Nacerin automatically gathers and produce aragonite

We will further search :
1) How CaCO3 makes aragonite
2) Conditions for gloss
3) Exact mechanism of making pearl by pearl shell
4) Method of making layers
5) Accurate role of colchiolin and possibility of producing it.

2. Human Practice
- Undergraduate essay contest : K water, Bioneer challenge, Samsung human-tech

June

Things to do
- Decide team logo & Order team T-shirt
- Homepage making (Contents)
- Festival at Yongsan youth center : Dongha Kim, Hyunsong Kim, Jihee Park

July

1. New name for Korea University biosynthetic academy
- Korea University Association of Synthetic biology (KUAS)

2. Role allocation
1) Sponsor :
in the school - Jihoon Jeong
outside the school - Dongha Kim, Junhong Jang, Jonghee Chun
2) Writing reports : Juyoung Han
3) CCP : Jihee Park, Hyunsong Kim, Juyoung Han, Minseob Yoo
4) Plans in Hongkong : Jihoon Jeong
5) Translation into English : Hyunsong Kim
6) Wiki contents : Minseob Yoo, Kyengwoo Jang, Jaeuk Han

August

1. Individual tasks
1) Jihoon Jeong - Report, Plane ticket, Wiki contents
2) Jaeuk Han - Wiki contents, Experiment
3) Hyeyeong Choi - Human Practice in Africa
4) Jihee Park - CCP, Experiment, Travel plan
5) Juyoung Han - Report, Design, Manager
6) Kyengwoo Jang - Wiki contents, Experiment
7) Junhong Jang - Experiment, Sponsor
8) Jonghee Chun - Sponsor, Homepage
9) Minseob Yoo - Experiment, CCP, Wiki contents
10) Dongha Kim - Homepage, Wiki platform
11) Minwhan Yoo - Homepage, wiki platform
12) Hyunsong Kim - Translation, Experiment, CCP

* Experiment day of week
- Monday : Jaeuk Han
- Tuesday : Jihee Park
- Wednesday : Juyoung Han
- Thursday : Hyunsong Kim

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-                    Background and Abstract
+                    March
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+             <p><br/><span>1. Introduction to iGEM</span><br/><span>- About synthetic biology : As an automobile can be described as assembly of numerous parts, synthetic biology is a field of assembled techniques from division of biotechnology. One of benefits offered in this field is that we can use necessary parts with lack of knowledge to specifics of each technique.</span><br/><span>- Look around 2012 iGEM teams' posters, wiki pages</span><br/><span></span><br/><span></span><br/><span>2. Division of roles</span><br/><span>1) Experiment : Minseob Yoo, Hyeyeong Choi, Junhong Jang, Hyunsong Kim, Jihee Park, Juyoung Ha</span><br/><span>2) Human Practice : Hyunsong Kim, Jihee Park, Hyeyeong Choi, Minseob Yoo</span><br/><span>3) Creative Challenger Program (CCP) : Juyoung Han, Minseob Yoo, Hyunsong Kim</span><br/><span>4) Design : Minseob Yoo</span><br/><span>5) Modeling : Dongha Kim, Minwhan Yoo, Jonghee Chun</span><br/><span>6) Fundraising : Kyengwoo Jang, Dongha Kim, Junhong Jang, Jihee Park</span><br/><span>7) Clerk : Hyunsong Kim</span><br/><span>8) Photographer : Jihoon Jeong</span><br/><span></span><br/><span></span><br/><span>3. Fix meeting dates</span><br/><span>- Meeing will be on every Monday, 7:00 p.m, lecture room 121 Life Science Division West building</span><br/><span></span><br/><span></span><br/><span>4. Studying iGEM</span><br/><span>- All members choose one team's result from past iGEM and make a presentation about it.</span><br/><span></span><br/><span>1) Jihoon Jeong - 2012 Cornell</span><br/><span>2) Jaeuk Han - 2012 Slovenia</span><br/><span>3) Hyeyeong Choi - 2011 Hong Kong-CUHK</span><br/><span>4) Jihee Park - 2011 Brown-Stanford</span><br/><span>5) Juyoung Han - 2012 PENN</span><br/><span>6) Kyengwoo Jang - 2012 Caltech</span><br/><span>7) Junhong Jang - 2012 Kyoto</span><br/><span>8) Jonghee Chun - 2012 Arizona state</span><br/><span>9) Minseob Yoo - 2012 Calgary</span><br/><span>10) Dongha Kim - 2012 Groningen</span><br/><span>11) Minwhan Yoo - 2011 Washington</span><br/><span>12) Hyunsong Kim - 2012 Carnegie Mellon</span><br/><span></span><br/><span></span><br/><span>5. Brainstorming for ideas</span><br/><span></span><br/><span></span><br/><span>6. Human Practice</span><br/><span>- Ask information agency about financial support and writing a newspaper article</span><br/><span>- Participating in Science fair at COEX</span><br/><span></span><br/><span>Hyeyeong Choi : Collaborate with Korea University club "Uhai" by making a presentation about Synthetic Biology at Africa</span><br/><span>Jihee Park: Making video introducing Synthetic Biology for elementary, middle and high school students</span><br/><span>Juyoung Han : Producing cartoon that expains Synthetic Biology</span><br/><span>Minwhan Yoo : Web-page production for KUAS(Korea University Association for Synthetic biology) beyond iGEM wiki page</span><br/><span>Dongha Kim : Making flash gamem Manufacturing circuit board</span><br/><span>Jonghee Chun : Homepage making</span><br/><span>Junhong Jang : Realization of biobrick by flash</span>
-            The global warming is the serious problem todays. It's the phenomenon which methane, CO2 and other green-house gases, caused from industrialization, increase the average temperature. These days, global warming causes abnormal climate; we should realize the seriousness of the situation and strive to decrease CO2. CCU (Carbon Capture and Utilization) is one of these efforts. It's the technique of collecting CO2 from power plant, steeling mill and others, converting CO2 into hydrocarbon and using it.<br /><br />
-            The Korea_U_Seoul team makes pearl-coli from this aspect. Pearl-coli is an <i>E.coli</i> converting CO2 into a pearl powder (nacre). The Korea_U_Seoul team aims to design Pearl-coli, an <i>E.coli</i> has an ability to convert atmospheric CO2 into pearl powder materials. The design is based on cell surface display of nacrein in <i>E.coli</i>. Nacrein is a major protein component in nacre(an organic-inorganic composite layer found in outer coating of pearls). We divided nacrein into functional regions - carbonic anhydrase(CA), calcium binding and scaffold repeats. CA domain fixes CO2 into carbonic acid changing bicarbonate ion in aqueous solution. We will examine if displayed nacrein in <i>E.coli</i> can make a pearl powder in a solution or fabricate a nacre-like structure while atmospheric CO2 is fixed into bicarbonate. Once a nacre material can be prepared from Pearl-coli, we will grow <i>E.coli</i> in a confined container to make synthetic pearl. The Pearl-coli has dual-function: mitigating the global warming by CO2 reduction and preparing valuable pearl-like raw materials.
-                <img src="https://static.igem.org/mediawiki/2013/5/59/Co2avcd.png">
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-                    Description
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-                 <img src="https://static.igem.org/mediawiki/2013/d/dd/CO2_dpa.jpg">
+                 <p><span>1. Ideas</span><br/><span>(1) Keywords&nbsp;</span><br/><span>- We made bulletin board at our website that we can easily search ideas</span><br/><span></span><br/><span>Kyengwoo Jang : Cyanobacteria+Rhodopsin, Acetic acid</span><br/><span>Junhong Jang : MSG</span><br/><span>Hyunsong Kim : Shwanella+Nano Silver</span><br/><span>Juyoung Han : Aluminum</span><br/><span>Jihoon Jeong : Proteorhodopsin</span><br/><span>Jihee Park : Endospore inner membrane</span><br/><span>Hyeyeong Choi : Food waste odor, Nuclear waste breakdown</span><br/><span></span><br/><span>(2) Teamwork</span><br/><span>-We made team to think systemically</span><br/><span></span><br/><span>Group 1 (Jihoon Jeong, Minseob Yoo) : CaCO3, AR system</span><br/><span>Group 2 (Jihee Park, Hyeyeong Choi, Kyengwoo Jang, Minseob Yoo, Juyoung Han) : Nonylphenol, SPR sensor, Lignin &beta;-keto adipate pathway</span><br/><span>Group 3 (Junhong Jang, Kyengwoo Jang, Hyunsong Kim, Juyoung Han) : Bio-hoil</span><br/><span>Group 4 (Jihoon Jeong, Jaeuk Han, Minseob Yoo, Dongha Kim) : Bio-wire</span><br/><span></span><br/><span>We consolidated group 3&amp;4 to the topic of nano metal producing by microbes</span><br/><span></span><br/><span></span><br/><span>2. Human Practice</span><br/><span>- Vote for Best 3 Human Practice from last year : Cornell, Calgary, LMU-Munich</span></p>
-                Our Team project is making pearl by E.coli induced Nacrein gene. At first, amplify kinds of nacrein genes by DH5a. Next, transformation of these genes to BW25113, BL21(DE3). Then nacrein genes make nacrein proteins. Finally they make nacrein aragonite structures itself and they forms the pearl.<br /><br />
+            </p>
-                Nacrein has carbonic anhydrase domain that can make CO2 to carbonic acid, and it changes to bicarbonate ion(HCO3-) and H+. And We use Nacrein, Nacrein CA, Nacrein DR, Nacrein R gene to check what gene can make lots of pearl. (CA, DR and R indicate first carbonic anhydrase domain clone, repeat deleted-clone and only repeat clone, respectively.) We can make pearl with reducing CO2.<br /><br />
+            </div>
-                 The Korea_U_Seoul team aims to design Pearl-coli that is <i>E.coli</i> able to convert atmospheric CO2 into a pearl powder material. The design is based on cell surface display of nacrein in <i>E.coli</i>. Nacrein is a major protein component in nacre (an organic-inorganic composite layer found in outer coating of pearls). We divided nacrein into several functional regions - carbonic anhydrase (CA), calcium binding and scaffold repeats. Carbonic anhydrase domain fixes CO2 into carbonic acid changing to bicarbonate ion in aqueous solution. We assume that the other regions except CA is related to formation of nacre layer in a pearl. The whole nacrein and those functional regions (CA, NR...??) are displayed in cell surface of <i>E.coli</i>. We will examine if displayed nacrein in <i>E.coli</i> can make a pearl powder in a solution or fabricate a nacre-like structure while atmospheric CO2 is fixed into bicarbonate. Once a nacre material can be prepared from Pearl-coli, we will grow <i>E.coli</i> in a confined container to make synthetic pearl. The Pearl-coli has dual-function such as (1) mitigate the global warming by CO2 reduction, (2) prepare valuable pearl-like raw materials.
+            <div class="title">
+                <div class="title_">
+                   May
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+            <p>
+                <p><span>1. Decide topic</span><br/><span>- Minseob Yoo</span><br/><span>: conchiolin is major protein of pearl</span><br/><span>There are two types of crystal structures of CaCO3, calcite and aragonite</span><br/><span>Nacerin automatically gathers and produce aragonite</span><br/><span></span><br/><span>We will further search :</span><br/><span>1) How CaCO3 makes aragonite</span><br/><span>2) Conditions for gloss</span><br/><span>3) Exact mechanism of making pearl by pearl shell</span><br/><span>4) Method of making layers</span><br/><span>5) Accurate role of colchiolin and possibility of producing it.</span><br/><span></span><br/><span></span><br/><span>2. Human Practice</span><br/><span>- Undergraduate essay contest : K water, Bioneer challenge, Samsung human-tech</span></p>
+            </p>
+            </div>
+            <div class="title">
+                 <div class="title_">
+                   June
+                </div>
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+            </div>
+            <div class="content_">
+            <p>
+                <p><span>Things to do</span><br/><span>- Decide team logo &amp; Order team T-shirt</span><br/><span>- Homepage making (Contents)</span><br/><span>- Festival at Yongsan youth center : Dongha Kim, Hyunsong Kim, Jihee Park</span></p>
+            </p>
+            </div>
+            <div class="title">
+                <div class="title_">
+                   July
+                </div>
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+            </div>
+            <div class="content_">
+            <p>
+                <p><span>1. New name for Korea University biosynthetic academy</span><br/><span>- Korea University Association of Synthetic biology (KUAS)</span><br/><span></span><br/><span>2. Role allocation</span><br/><span>1) Sponsor :</span><br/><span>in the school - Jihoon Jeong</span><br/><span>outside the school - Dongha Kim, Junhong Jang, Jonghee Chun</span><br/><span>2) Writing reports : Juyoung Han</span><br/><span>3) CCP : Jihee Park, Hyunsong Kim, Juyoung Han, Minseob Yoo</span><br/><span>4) Plans in Hongkong : Jihoon Jeong</span><br/><span>5) Translation into English : Hyunsong Kim</span><br/><span>6) Wiki contents : Minseob Yoo, Kyengwoo Jang, Jaeuk Han</span></p>
+            </p>
+            </div>
+            <div class="title">
+                <div class="title_">
+                   August
+                </div>
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+            </div>
+            <div class="content_">
+            <p>
+                <p><span>1. Individual tasks</span><br/><span>1) Jihoon Jeong - Report, Plane ticket, Wiki contents</span><br/><span>2) Jaeuk Han - Wiki contents, Experiment</span><br/><span>3) Hyeyeong Choi - Human Practice in Africa</span><br/><span>4) Jihee Park - CCP, Experiment, Travel plan</span><br/><span>5) Juyoung Han - Report, Design, Manager</span><br/><span>6) Kyengwoo Jang - Wiki contents, Experiment</span><br/><span>7) Junhong Jang - Experiment, Sponsor</span><br/><span>8) Jonghee Chun - Sponsor, Homepage</span><br/><span>9) Minseob Yoo - Experiment, CCP, Wiki contents</span><br/><span>10) Dongha Kim - Homepage, Wiki platform</span><br/><span>11) Minwhan Yoo - Homepage, wiki platform</span><br/><span>12) Hyunsong Kim - Translation, Experiment, CCP</span><br/><span></span><br/><span>* Experiment day of week&nbsp;</span><br/><span>- Monday : Jaeuk Han</span><br/><span>- Tuesday : Jihee Park</span><br/><span>- Wednesday : Juyoung Han</span><br/><span>- Thursday : Hyunsong Kim</span></p>
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