Team:Washington/appsetup

From 2013.igem.org

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<b><u>How to start the app</u></b></br>
<b><u>How to start the app</u></b></br>
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Changing Intensity</br>
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<b>Changing Intensity</b></br>
1. Follow step 1 to 4 on the protocol above.</br>
1. Follow step 1 to 4 on the protocol above.</br>
2. Change intensity between 0% to 100%. (0% is completely dark)</br>
2. Change intensity between 0% to 100%. (0% is completely dark)</br>
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3. Follow step 5 to 6 on the protocol above.</br></br>
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<b>Blinking</b></br>
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3. Follow step 5 to 6 on the protocol above.</br>
 
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<b><u>Blinking</b></u></br>
 
1. Follow step 1 to 4 on the protocol above.</br>
1. Follow step 1 to 4 on the protocol above.</br>
2. Change the Green Wait (Milliseconds) to 125 and Black Wait (Milliseconds) to 875 to have alternating color expression the wells. You may vary the time depending on your experiment from 0 millisecond to 60000 milliseconds maximum</br>
2. Change the Green Wait (Milliseconds) to 125 and Black Wait (Milliseconds) to 875 to have alternating color expression the wells. You may vary the time depending on your experiment from 0 millisecond to 60000 milliseconds maximum</br>
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Latest revision as of 21:18, 27 September 2013



How to start the app
1. Start the E.colight app.
2. Select from petri dish (100 mm or 60 mm) or 96 well plate format
For 60mm petri dishes, go to step 3A. For 96 well plate, skip to step 3B.

Washington app picture1.jpg
























3A. Use your finger to highlight the appropriate wells. To make green wells refer to step 4A, for dark wells, refer to step 4B.

Washington app picture2.jpg
























3B. Use your finger to highlight the appropriate wells. To make green wells refer to step 4A, for dark wells, refer to step 4B.

Washington app picture3.jpg
























4A. Press 'Set Values'. Set Red Wait (milliseconds) to 0. Set Green Wait (milliseconds) to 200. Set Blue Wait (Milliseconds) to 0. Set Black Wait (milliseconds) to 0. To change intensity or to setup blinking experiments refer to supplemental.

Washington app picture4.jpg
























4B. Press 'Set Values'. Set Red Wait (milliseconds) to 0. Set Green Wait (milliseconds) to 0. Set Blue Wait (Milliseconds) to 0. Set Black Wait (milliseconds) to 200. To change intensity or to setup blinking experiments refer to supplemental.

Washington app picture5.jpg
























5. Press 'Set Values' to return to the experiment screen.

Washington app picture6.jpg

Washington app picture7.jpg
























6. Press 'Start Test'

Supplemental:

Changing Intensity
1. Follow step 1 to 4 on the protocol above.
2. Change intensity between 0% to 100%. (0% is completely dark)

Washington app picture8.jpg
























3. Follow step 5 to 6 on the protocol above.

Blinking
1. Follow step 1 to 4 on the protocol above.
2. Change the Green Wait (Milliseconds) to 125 and Black Wait (Milliseconds) to 875 to have alternating color expression the wells. You may vary the time depending on your experiment from 0 millisecond to 60000 milliseconds maximum

Washington app picture9.jpg
























3. Follow step 5 to 6 on the protocol above.