Team:Hong Kong CUHK

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   <h1>Abstract</h1>
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  <p><u>Switch off PAHs</u></p>
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   <p>Polycyclic  aromatic hydrocarbons (PAHs) are harmful to both environment and human health.  We proposed the PAHs degradation system, which contains codon-optimized laccase  from Bacillus sp. HR03 and catechol 1,2-dioxygenase from Pseudomonas putida  KT2440 for Escherichia coli...</p>
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   <p2>To rapidly regulate biological process, we designed a novel transmembrane protein called Voltage Switch (VS), which is a fusion protein utilizing the voltage sensing domain from potassium ion channels. Triggered by a change of potential across the inner membrane, VS can bring targeting enzymes into proximity, thus allowing an instant control of enzymatic reaction...</p2>
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   <p>An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will place them in the Registry of Standard Biological Parts...</p>
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   <h1>Human Practice</h1>
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  <p2>Interest and mindset formation should start at young  age for permanence and  continuity. High school and university students are  great audiences  to be  approached to break the blurred paradigm of synthetic  biology, which term is rarely  known by the general public...</p2>
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  <p>The system  was capable of degrading PAHs into less toxic simple carboxylic acid. Also,  since quinones are intermediates in the pathway, we added quinone sensing and  response repressor (QsrR) to regulate the degradation process. To accelerate  the degradation, we used Voltage Switch (VS), the other great highlight of our  project. We designed a novel transmembrane protein called Voltage Switch (VS),  which is a fusion protein utilizing the voltage sensing domain from potassium  ion channels. Triggered by change in potential across the cell membrane, VS can  separate or bring targeting enzymes into proximity; thus allowing an instant  control of enzymatic reaction. </p>
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<div id="partunder" onmouseout="MM_showHideLayers('PAHsurface','','show','PAHunder','','hide')"><p1> The iGEM software provides an easy way to present the parts your team has created . The &quot;groupparts&quot; tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the Registry, not on your team wiki.</p1>
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   <h1>Voltage Switch</h1>
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   <p>Another great highlight of our project is Voltage Switch. In order to increase the efficiency of PAHs degradation pathway, we design a novel switch triggered by electricity. The switch includes differently combined protein domains that regulate the distance between enzymes.</p>
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   <p2>Voltage Switch is a response system triggered by a change of potential across the bacterial inner membrane. Components of this system include the transmembrane voltage sensor peptide originated from potassium ion channels, polyproline linker in the inner membrane, glycine serine linker, PDZ domain and PDZ ligand in the periplasm, and glycine serine linker and effectors in the cytosol...</p2>
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   <h1>PAH Degradation</h1>
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   <p>We propose a pathway of PAH degradation, which is carried out by a combination of enzyme laccase and catechol 1, 2-dioxygenase....<br />
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   <p2>Our project initiated from dealing with toxic chemicals found in second-hand smoke (or environmental  tobacco smoke, ETS) and cooking fume. After carefully examining the composition  of pollutes, we noticed that there was one group of highly toxic...</p2>
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  It allows fast control of concentration of reaction mixture, thus achieving fast response by electricity. Apart from using Voltage Switch, we also try to link the two enzymes together as a reaction complex by Protein Scaffolding, the project from SJTU 2012 iGEM team.</div>
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<div id="PAHunder" onmouseout="MM_showHideLayers('BGPAH','','show','BGPAHunder','','hide')"><p1></p1><p> The final product is a kind of simple carboxylic acid, which is less harmful to human body. Since quinones are generated in the beginning of the metabolism, we use quinone sensing and response repressor (QsrR) to control the degradation process.</p>
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   Address: Rm. 184, Science Centre, CUHK <br/>
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   Address: Room 184, Science Centre, CUHK <br/>
   Email: kingchan@cuhk.edu.hk  Tel: (852)-39434420  Fax: (852)-26037246
   Email: kingchan@cuhk.edu.hk  Tel: (852)-39434420  Fax: (852)-26037246
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Latest revision as of 02:10, 29 October 2013

iGEM CUHK

Retrieved from "http://2013.igem.org/Team:Hong_Kong_CUHK"