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Revision as of 22:01, 27 September 2013 (view source) Mague (Talk | contribs) ← Older edit		Latest revision as of 02:57, 28 September 2013 (view source) Gabyqz (Talk | contribs)
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		+	[[File:Piezasgens.jpg|right|300px]]
		+	Plasmid extraction from synthetized parts
		+	pUC57: 2710 bp
	*In the lane 1 is the ladder of 5000 bp		*In the lane 1 is the ladder of 5000 bp
-	*The sample of the lane 3 is the promoter 1	+	*The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
-	*The sample of the lane 5 is the promoter 5	+	*The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
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		+	*The sample of the lane 7 is the RBS, inserted sequence: 76 bp
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		+	----
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		+	[[File:Piezas_PsB1C3.jpg|right|300px]]
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		+	Parts clonning in pSB1C3: 2070 bp
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		+	*In the lane 1 is the ladder of 3000 bp
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		+	*The sample of the lane 3 is the promoter 2
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		+	*The sample of the lane 4 is the promoter 1
	*The sample of the lane 7 is the RBS		*The sample of the lane 7 is the RBS
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		+	----
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		+	[[File:Plasmid_GFP.jpg|right|300px]]
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		+	Ligation Promoters with GFP
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		+	*In the lane 1 is the ladder of 5000 bp
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		+	*The sample of the lane 3 is the Plasmid GFP
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		+	*The sample of the lane 5 is the Plasmid GFP
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		+	----
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		+	[[File:P1_expression.jpg|center|300px]]
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		+	GFP expression for L.Plantarum promoter cloned in E.coli
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		+	----
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		+	[[File:P2_expression.jpg|center|300px]]
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		+	GFP expression for L.Plantarum promoter 2 cloned in E.coli

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Latest revision as of 02:57, 28 September 2013

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Results

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Plasmid extraction from synthetized parts

pUC57: 2710 bp

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp

• The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp

• The sample of the lane 7 is the RBS, inserted sequence: 76 bp

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Parts clonning in pSB1C3: 2070 bp

• In the lane 1 is the ladder of 3000 bp

• The sample of the lane 3 is the promoter 2

• The sample of the lane 4 is the promoter 1

• The sample of the lane 7 is the RBS

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Ligation Promoters with GFP

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the Plasmid GFP

• The sample of the lane 5 is the Plasmid GFP

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GFP expression for L.Plantarum promoter cloned in E.coli

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GFP expression for L.Plantarum promoter 2 cloned in E.coli