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Team:Washington/60mmPetriDishAssay
From 2013.igem.org
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| - | + | Obtain <a href = "http://catalog2.corning.com/Lifesciences/en-US/Shopping/ProductDetails.aspx?productid=430589(Lifesciences)&categoryname=">60 mm Petri Dish</a>.</br> | |
1. Add 7.5 ml of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> above into two 60 mm petri dishes. </br> | 1. Add 7.5 ml of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> above into two 60 mm petri dishes. </br> | ||
2. Cover one of the 60 mm petri dishes with foil.</br> | 2. Cover one of the 60 mm petri dishes with foil.</br> | ||
Latest revision as of 22:28, 27 September 2013
60 mm Petri Dish Assay Obtain 60 mm Petri Dish. 1. Add 7.5 ml of the overnight culture prepared according to the general protocol above into two 60 mm petri dishes. 2. Cover one of the 60 mm petri dishes with foil. 3. Secure the foiled petri dish with tape on top of the orbital shaker. 4. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish. 5. Place the stacked plates such that the bottom of the spacer plate aligns with the lighted circle in the app. 6. Secure the plates with tape. Incubate the tablet and dishes on an orbital shaker at 140 RPM for 16 hours at 37C 7. Follow the Fluorescent Analysis Protocol.
