Team:Shenzhen BGIC ATCG/parts

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:57, 28 October 2013

Playing with my eyes
aren't you?

Hi I am Dr. Mage!
A "budding" yeast cell!

Best BioBricks
Devices List
Basic Parts
Parts Sequencing

Best BioBricks

Best BioBrick (Natural/Collection), BBa_K1051303, BBa_K1051300 ~ BBa_K1051306

K1051303

K1051300

K1051301

K1051302

K1051304

K1051305

K1051306

Best BioBrick (Engineered), BBa_K1051900

K1051900

Best Measurement, BBa_K1051257 ~ BBa_K1051259

K1051257

K1051258

K1051259

Devices List

Final Constructions

Final Constructions of E.coli

K1051910

K1051911

K1051912

Final Constructions of Budding Yeast

K1051900a

K1051900b

K1051901

K1051902

K1051903

Promoter Verification

Verification of E.coli

K1051245

K1051246

K1051247

Verification of Budding Yeast

K1051307

K1051355

K1051356

K1051357

K1051358

K1051359

K1051365

K1051366

K1051367

K1051368

K1051369

Degradation Rate Measurement

Measurement of E.coli

K1051257

K1051258

K1051259

Measurement of Budding Yeast

K1051278

K1051279

K1051280

K1051281

K1051288

Targeting Peptides Verification

K1051150

K1051151

K1051152

K1051153

CRISPRi Verification

K1051852

Alternative Splicing Verification

K1051706

Basic Parts

Universal Parts

Reporters

Name	Type	Description	Designer	Length
BBa_K1051000	Reporter	Stop codon free RFP in RFC[23] standard	Xiang LI	675
BBa_K1051001	Reporter	Stop codon free ECFP in RFC[23] standard	Xiang LI	716
BBa_K1051002	Reporter	Stop codon free EYFP in RFC[23] standard	Xiang LI	717
BBa_K1051003	Reporter	Stop codon free GFP in RFC[23] standard	Xiang LI	714
BBa_K1051004	Reporter	Stop codon free mOrange in RFC[23] standard	Xiang LI	738

Yeast Terminators

Name	Type	Description	Designer	Length
BBa_K1051006	Terminator	Saccharomycescerevisiae TBY-1 terminator with stopcodon	Xiang LI	31
BBa_K1051007	Terminator	S. cerevisiae SSV7 terminator with stopcodon	Xiang LI	39
BBa_K1051008	Terminator	S. cerevisiae CAA terminator with stopcodon	Xiang LI	18

E. coli Terminators

Name	Type	Description	Designer	Length
BBa_K1051009	Terminator	e.coli terminator ilvGEDA_T with stop codon	Xiang LI	92

Cyclin Promoters

Yeast

Name	Type	Description	Designer	Length
BBa_K1051300	Regulatory	cln2 promoter (G1 phase to S phase)	Kequan Lin	1000
BBa_K1051301	Regulatory	clb2 promoter (during G2 phase)	Kequan Lin	1063
BBa_K1051302	Regulatory	clb5 promoter (during G1 phase)	Kequan Lin	648
BBa_K1051304	Regulatory	cln3 promoter (M phase to G1 phase)	Kequan Lin	1075
BBa_K1051305	Regulatory	met16 promoter (M phase to G1 phase)	Zhu Shuang	551
BBa_K1051306	Regulatory	met28 promoter (S phase)	Zhu Shuang	500

E. coli

Name	Type	Description	Designer	Length
BBa_K1051400	Regulatory	promoter->dnaAp1	Bingwei Zheng	359
BBa_K1051401	Regulatory	promoter->dnaAp1 no RBS	Bingwei Zheng	184
BBa_K1051402	Regulatory	promoter->gyrB	Bingwei Zheng	188
BBa_K1051403	Regulatory	promoter->gyrB no RBS	Bingwei Zheng	158
BBa_K1051404	Regulatory	promoter->ftsQp	Bingwei Zheng	485
BBa_K1051405	Regulatory	promoter->ftsQp no RBS	Bingwei Zheng	198

Degradation Tags

Yeast

Name	Type	Description	Designer	Length
BBa_K1051200	DNA	ubiquitin	wanling ZHOU	1146
BBa_K1051201	DNA	D-BOX	wanling ZHOU	39
BBa_K1051202	DNA	cln1-PEST	wanling ZHOU	489
BBa_K1051203	DNA	Cln2-PEST	wanling ZHOU	537
BBa_K1051204	DNA	cln3-PEST	wanling ZHOU	588

E. coli

Name	Type	Description	Designer	Length
BBa_K1051206	Tag	The degradation tag in E. coil ,M0050 with TAATAA.	Rui Guan	39
BBa_K1051207	Tag	The degradation tag in E. coil ,M0051 with TAATAA.	Rui Guan	45
BBa_K1051208	Tag	The degradation tag in E. coil ,M0052 with TAATAA.	Rui Guan	39

Targeting Peptides

Mitochondria

Name	Type	Description	Designer	Length
BBa_K1051100	Coding	targeting peptide to mitochondria inner membrane	Jinchun Zhang	209
BBa_K1051101	Coding	Cox4 targeting peptide to mitochondria inner membrane	Jinchun Zhang	75
BBa_K1051102	Coding	targeting peptide to mitochondria inner membrane	Jinchun Zhang	141
BBa_K1051103	Coding	TOM22, targeting peptide to mitochondria outer membrane	Jinchun Zhang	69
BBa_K1051104	Coding	VDAC1;targeting peptide to mitochondria outer membrane	Jinchun Zhang	93
BBa_K1051105	Coding	TOM40;targeting peptide to mitochondria outer membrane	Jinchun Zhang	147
BBa_K1051106	Coding	targeting peptide to mitochondria outer membrane	Jinchun Zhang	96
BBa_K1051107	Coding	Tom70;targeting peptide to mitochondria outer membrane	Jinchun Zhang	90
BBa_K1051108	Coding	targeting peptide to mitochondria outer membrane;Tom20	Jinchun Zhang	120
BBa_K1051109	Coding	targeting peptide to mitochondria matrix;MIA40	Jinchun Zhang	93

Centrosome

Name	Type	Description	Designer	Length
BBa_K1051110	Coding	ScSfi1;targeting peptide to centrosome	Jinchun Zhang	63
BBa_K1051111	Coding	ScSfi1E; targeting peptide to centrosome;	Jinchun Zhang	63
BBa_K1051112	Coding	ScKar1;targeting peptide to centrosome	Jinchun Zhang	60
BBa_K1051113	Coding	ScSac3;targeting peptide to centrosome	Jinchun Zhang	57

Nucleus

Name	Type	Description	Designer	Length
BBa_K1051114	Coding	H2A2;targeting peptide to nucleus	Jinchun Zhang	408

Vacuolar

Name	Type	Description	Designer	Length
BBa_K1051115	Coding	ZRC1;Vacuolar Membrane Targeting Protein	Jinchun Zhang	396

Actin

Name	Type	Description	Designer	Length
BBa_K1051116	Coding	Actin Targeting Protein	Jinchun Zhang	1779

Plasma Membrane

Name	Type	Description	Designer	Length
BBa_K1051117	Coding	Plasma Membrane Targeting Sequence	Jinchun Zhang	12

Peroxisomes

Name	Type	Description	Designer	Length
BBa_K1051118	Coding	ScPEX21p；baking yeast targeting peptide to peroxisome ;	Jinchun Zhang	30

CRISPRi Verification

Name	Type	Description	Designer	Length
BBa_K1051800	Coding	protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.	Shihong Chen	4164
BBa_K1051801	Device	Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib	Shihong Chen	388
BBa_K1051801	Device	Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib	Shihong Chen	388

Alternative Splicing Verification

Name	Type	Description	Designer	Length
BBa_K1051700	DNA	SRC1 Intron -4bp at 5'	Yang Zhou	129
BBa_K1051701	DNA	SRC1 Intron+GG	Yang Zhou	132
BBa_K1051702	DNA	SRC1 Intron+CGG	Yang Zhou	133
BBa_K1051703	DNA	SRC1 Intron with additional 6bp at both directions	Yang Zhou	142

Parts Sequencing

All of our parts have been sequenced to get verification.

Sequencing example of K1051701

Figure. Sequencing Result

Alignment example of K1051701

Figure. Alignment Result

@@ Line 161: / Line 161: @@
 <h4>Final Constructions</h4>
 <h5>Final Constructions of <i>E.coli</i></h5>
-<p>K1051410</p>
+<p>K1051910</p>
-<img src="https://static.igem.org/mediawiki/2013/f/f5/K1051410.png">
+<img src="https://static.igem.org/mediawiki/2013/d/d7/K1051910.png">
-<p>K1051412</p>
+<p>K1051911</p>
-<img src="https://static.igem.org/mediawiki/2013/8/8d/K1051412.png">
+<img src="https://static.igem.org/mediawiki/2013/0/0a/K1051911.png">
-<p>K1051414</p>
+<p>K1051912</p>
-<img src="https://static.igem.org/mediawiki/2013/f/fa/K1051414.png">
+<img src="https://static.igem.org/mediawiki/2013/b/bc/K1051912.png">
 <h5>Final Constructions of Budding Yeast</h5>
 <p>K1051900a</p>