Team:Berkeley/Safety

From 2013.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Berkeley/header}}
{{Team:Berkeley/header}}
-
{{Team:Berkeley/navigation}}
 
<html>
<html>
-
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Template:Team:Berkeley/foundation?action=raw&ctype=text/css" />
+
   
-
<link rel="stylesheet2" type="text/css" href="https://2013.igem.org/Template:Team:Berkeley/css?action=raw&ctype=text/css" />
+
    <body data-spy="scroll" data-target="#navbar">
-
<div class = "content">
+
        <div id="navbar">
-
<div class = "container">
+
            <ul class="nav">
 +
                <li id="TitleID"> <a href="https://2013.igem.org/Team:Berkeley/Safety" id="TitleID">Safety</a>  
 +
                </li>
 +
                <li><a href="#1">Safety Overview</a>
-
<div class = "heading"><a name="Undergraduates">Safety Overview</a></div> <br />
+
                </li>
-
All of our team members were given a thorough safety training prior to the start of the iGEM program. A list of training programs that we attended can be found here http://rac.berkeley.edu/training.html. UC Berkeley EH&S office (link to eh s) closely supervises research practices on campus, and our project was first approved by this office before its commencement.
+
                <li><a href="#2">Chassis Organisms</a>
-
<!-- DO NOT ERASE -->
+
-
**Safety forms were approved on 9/18/13 by David Lloyd and Julie McNamara. <br>
+
-
<div class = "heading"><a name="EconomicalAnalysis">Chassis Organisms</a></div> <br />
+
                </li>
-
<br> Our team worked only with the Escherichia Coli and Saccharomyces Cerevisiae species. E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). S.cerevisiae is a GRAS organism (Generally recognized as Safe). Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity (http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm). Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks. </br>
+
                <li><a href="#3">Biobrick Part Safety</a>
-
<div class = "heading"><a name="EconomicalAnalysis">Biobrick Part Safety</a></div> <br />
+
                </li>
 +
                <li><a href="#4">Dyeing with Indican-Safety</a>
-
We do not envision any of the parts we are using to be a safety concern in the lab. All of the parts we have cloned and expressed as well as their corresponding substartes and products have been documented previously by other authors. FMO's product, indigo, is known to be a low toxicity compound (http://www.ncbi.nlm.nih.gov/pubmed/3598883) and many of the safety practices surrounding use of indigo have been well documented in the literature.<br>
+
                </li>
 +
            </ul>
 +
        </div>
 +
        <div id="inner-content">
 +
            <div class="heading-large"><a>Safety</a>
-
<div class = "heading"><a name="EconomicalAnalysis">Dyeing with Indican - Safety</a></div> <br />
+
            </div>
 +
            <div id="1">
 +
                <div class="heading"><a name="Undergraduates">Safety Overview</a>
-
In our envisioned dyeing process, indican would be the primary chemical that would coat jeans. Once indican was adhered to the jeans, our B-glucosidase would be added either as cell culture or purified enzyme to the jeans, which would then turn indican into indigo. Once the jeans have been sufficiently dyed, we have two priorities to ensure safety: removing any trace of bacteria from the cloth and removing indican. Indigo has already been proved to be safe to handle by both industry and labs. To remove both bacteria and indican, in the indican dyeing process, several rounds of washes in detergent as well as autoclaving the cloth would be ideal solutions. Because indican is soluble, it will wash off easily in detergent and water. E. Coli, the organism in which this biosynthetic dyeing pathway will be implemented, would also be killed by autoclaving because of the high temperatures. <br>
+
                </div>
-
<br>
+
                <p>
-
We followed our these outlined safety procedures in our own dyeing of lanyards with indican. We washed our lanyards with 5% SDS (a detergent) several times and then autoclaved the cotton material before washing again with 1% detergent water. <br>
+
                    <br />All of our team members were given a thorough safety training prior to the start of the iGEM program. A list of training programs that we attended can be found here http://rac.berkeley.edu/training.html. UC Berkeley EH&S office (http://www.ehs.berkeley.edu/) closely supervises research practices on campus, and our project was first approved by this office before its commencement.
-
<br>
+
                    <br>
-
Scaling our process would present the added safety concerns of building and maintaining a bioreactor facility. Any facility meant to grow indican-producing and B-glucosidase producing bacteria as outlined on our Human Practices page would follow standard bioreactor design regulations as well as waste and operation regulations. <br>
+
                    <br>Safety forms were approved on 9/18/13 by David Lloyd and Julie McNamara.
 +
                    <br>
 +
                    <br>
 +
                </p>
 +
            </div>
 +
            <div id="2">
 +
                <div class="heading"><a name="EconomicalAnalysis">Chassis Organisms</a>
 +
                </div>
 +
                <p>
 +
                    <br />Our team worked only with the Escherichia coli and Saccharomyces cerevisiae species. E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). S. cerevisiae is a GRAS organism (Generally recognized as Safe). Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity (http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm). Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks.</br>
 +
                    <br>
 +
                </p>
 +
            </div>
 +
            <div id="3">
 +
                <div class="heading"><a name="EconomicalAnalysis">Biobrick Part Safety</a>
 +
                </div>
 +
                <p>
 +
                    <br />We do not envision any of the parts we are using to be a safety concern in the lab. All of the parts we have cloned and expressed as well as their corresponding substartes and products have been documented previously by other authors. FMO's product, indigo, is known to be a low toxicity compound (http://www.ncbi.nlm.nih.gov/pubmed/3598883) and many of the safety practices surrounding use of indigo have been well documented in the literature.
 +
                    <br>
 +
                    <br>
 +
                </p>
 +
            </div>
 +
            <div id="4">
 +
                <div class="heading"><a name="EconomicalAnalysis">Dyeing with Indican - Safety</a>
 +
                </div>
 +
                <p>
 +
                    <br />In our envisioned dyeing process, indican would be used as a dyeing material. Once indican permeates fabric, our B-glucosidase would be added either as cell culture or purified enzyme to the jeans, which would then turn indican into indigo. Once the jeans have been sufficiently dyed, we would aim to: remove or eliminate bacteria from the cloth, remove or recycle unbound indigo, and remove or recycle unreacted indican. Indigo and indican are generally safe, though we would want to avoid large-scale environmental release. After dyeing fabric, several rounds of washes in detergent will likely eliminate any remaining bacteria. Our process, if scaled up to industrial levels, would have to undergo rigorous testing to confirm that the dyed product is cell-free. Because indican is soluble, it will wash off easily in detergent and water, and unbound indigo will likewise be removed.
 +
                    <br>
 +
                    <br>After dyeing our cotton bears with indican and our purified B-glucosidase, we washed with 5% SDS (a detergent) several times and then autoclaved the cotton material before washing again with 1% detergent water to be sure that we have completely eliminated all biological activity before removing them from the lab.
 +
                    <br>
 +
                    <br>Scaling our process would present the added safety concerns of building and maintaining a bioreactor facility. Any facility meant to grow indican-producing and B-glucosidase producing bacteria as outlined on our Human Practices page would follow standard bioreactor design regulations as well as waste and operation regulations.
 +
                    <br>
 +
                </p>
 +
            </div>
 +
 
 +
<script>
 +
var offset = 54;
 +
$('#navbar li a').click(function(event) {
 +
if ( ($(this).attr('id')) == 'TitleID') {
 +
return;
 +
}
 +
else {
 +
    event.preventDefault();
 +
    $($(this).attr('href'))[0].scrollIntoView();
 +
    scrollBy(0, -offset);
 +
}
 +
});
 +
</script>
 +
</html>

Latest revision as of 03:45, 29 October 2013

Safety
Safety Overview


All of our team members were given a thorough safety training prior to the start of the iGEM program. A list of training programs that we attended can be found here http://rac.berkeley.edu/training.html. UC Berkeley EH&S office (http://www.ehs.berkeley.edu/) closely supervises research practices on campus, and our project was first approved by this office before its commencement.

Safety forms were approved on 9/18/13 by David Lloyd and Julie McNamara.

Chassis Organisms


Our team worked only with the Escherichia coli and Saccharomyces cerevisiae species. E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). S. cerevisiae is a GRAS organism (Generally recognized as Safe). Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity (http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm). Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks.

Biobrick Part Safety


We do not envision any of the parts we are using to be a safety concern in the lab. All of the parts we have cloned and expressed as well as their corresponding substartes and products have been documented previously by other authors. FMO's product, indigo, is known to be a low toxicity compound (http://www.ncbi.nlm.nih.gov/pubmed/3598883) and many of the safety practices surrounding use of indigo have been well documented in the literature.

Dyeing with Indican - Safety


In our envisioned dyeing process, indican would be used as a dyeing material. Once indican permeates fabric, our B-glucosidase would be added either as cell culture or purified enzyme to the jeans, which would then turn indican into indigo. Once the jeans have been sufficiently dyed, we would aim to: remove or eliminate bacteria from the cloth, remove or recycle unbound indigo, and remove or recycle unreacted indican. Indigo and indican are generally safe, though we would want to avoid large-scale environmental release. After dyeing fabric, several rounds of washes in detergent will likely eliminate any remaining bacteria. Our process, if scaled up to industrial levels, would have to undergo rigorous testing to confirm that the dyed product is cell-free. Because indican is soluble, it will wash off easily in detergent and water, and unbound indigo will likewise be removed.

After dyeing our cotton bears with indican and our purified B-glucosidase, we washed with 5% SDS (a detergent) several times and then autoclaved the cotton material before washing again with 1% detergent water to be sure that we have completely eliminated all biological activity before removing them from the lab.

Scaling our process would present the added safety concerns of building and maintaining a bioreactor facility. Any facility meant to grow indican-producing and B-glucosidase producing bacteria as outlined on our Human Practices page would follow standard bioreactor design regulations as well as waste and operation regulations.

Retrieved from "http://2013.igem.org/Team:Berkeley/Safety"