|
|
| (21 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | <html>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| | | | |
| - |
| |
| - | <style type="text/css">
| |
| - | *{margin:0;padding:0;font-family:"微软雅黑","Arial";}
| |
| - |
| |
| - | body{
| |
| - | width: 960px;
| |
| - | height: auto;
| |
| - | margin: 0 auto;
| |
| - | font-size:12px;
| |
| - | font-family:Arial, Helvetica, sans-serif;
| |
| - | background-color:#fff;
| |
| - | }
| |
| - |
| |
| - | h1,h2,h3,h4,h5,h6{
| |
| - | border:none;}
| |
| - |
| |
| - | p {margin:0.5em 0 !important;
| |
| - | text-decoration:none;
| |
| - | font-family:Arial, Helvetica, sans-serif;
| |
| - | font-size:12px;
| |
| - | }
| |
| - | .content-section{
| |
| - | width:960px;
| |
| - | height:10px;
| |
| - | margin-top:0px;
| |
| - | }
| |
| - | .logo-section{
| |
| - | height:220px;
| |
| - | width:960px;
| |
| - | position:relative;
| |
| - | float:left;
| |
| - | margin-top:5px;
| |
| - | background:url(https://static.igem.org/mediawiki/igem.org/1/16/Tju-Project-banner.jpg) no-repeat;
| |
| - | }
| |
| - | .note{
| |
| - | width:960px;
| |
| - | height:200px;
| |
| - | float:left;
| |
| - | margin-top:10px;
| |
| - | position:relative;
| |
| - | background-color:#fff;
| |
| - | padding:0;
| |
| - | }
| |
| - | .note1{
| |
| - | width:960px;
| |
| - | min-height:200px;
| |
| - | float:left;
| |
| - | background-color:#fff;
| |
| - | margin-top:0px;
| |
| - | }
| |
| - | .note2{
| |
| - | width:880px;
| |
| - | height:auto;
| |
| - | float:left;
| |
| - | position:relative;
| |
| - | margin-left:20px;
| |
| - | padding:30px 10px 10px 30px;
| |
| - | }
| |
| - | #footer{
| |
| - | margin:0 auto;
| |
| - | width:960px;
| |
| - | min-height:73px;
| |
| - | text-align:center;
| |
| - | color:#666;
| |
| - | float:left;
| |
| - | }
| |
| - | #goTopBtn {POSITION: fixed;TEXT-ALIGN: center;LINE-HEIGHT: 30px;WIDTH: 100px;BOTTOM: 35px;HEIGHT: 100px;FONT-SIZE: 12px;RIGHT: 30px;}
| |
| - |
| |
| - |
| |
| - | <!--hidden section-->
| |
| - | .hidden {display:none;}
| |
| - | #p-logo,#footer-box {display:none;}
| |
| - | #top-section {border:none;height:15px;}
| |
| - | .firstHeading {display:none;}
| |
| - | #bodyContent{display:none;}
| |
| - | #menubar a{text-decoration:none;color:#CCC;list-style: none;}
| |
| - | #menubar a:hover{text-decoration:none;color:#FFF;list-style: none;}
| |
| - | #search-controls{display:none;}
| |
| - | .printfooter{display:none;}
| |
| - | #catlinks{display:none;}
| |
| - | #content{background:none;padding:0px;margin-top:0px;border:none;}
| |
| - | #toc{display:none;}
| |
| - | </style>
| |
| - |
| |
| - | <!--content section-->
| |
| - |
| |
| - | <style type="text/css">
| |
| - | .sddm{z-index: 30;width: 960px;height:65px;position:relative;float:left;background-color:#FFF;position:raletive;margin-left:0px;}
| |
| - | .sddm ul{margin-left:0px;}
| |
| - | .sddm li{margin: 0;list-style: none;float: left;font: bold 14px arial;height:25px;background-color:#FFF;}
| |
| - | .sddm li a{display: block;margin:0;padding: 15px 0px 15px 0px;width: 96px;background: #FFF;color: #000;text-align: center;text-decoration: none;border-top:#ececec 5px solid;}
| |
| - | .sddm li a:hover{background:#FCFCFC;color:#0babe7;border-top:#0babe7 5px solid;}
| |
| - | .sddm div{position: absolute;width:96px;z-index:999;visibility: hidden;margin: 0;padding: 0;}
| |
| - | .sddm div a{position: relative;display: block;margin: 0;padding: 5px 5px;width: auto;white-space: nowrap;text-align:center;text-decoration: none;color: #000;font: 12px arial}
| |
| - | .sddm div a:hover{background:#FAFAFA;color: #0babe7;}
| |
| - | </style>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div class="cont1">
| |
| - | <ul class="sddm">
| |
| - |
| |
| - | <style type="text/css">
| |
| - | .A-C{font-size:12px !important;padding-top:0 !important;text-align:center;line-height:100%}
| |
| - | .HP{font-size:14px !important;padding-top:0 !important;text-align:center;}
| |
| - | </style>
| |
| - |
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin" style="padding:20px 0px 15px 0px;height:30px;">Home</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Project" style="padding:20px 0px 15px 0px;height:30px;">Project</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Safety " style="padding:20px 0px 15px 0px;height:30px;">Safety</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Modeling" style="padding:20px 0px 15px 0px;height:30px;">Modeling</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Protocol" style="padding:20px 0px 15px 0px;height:30px;">Protocol</a>
| |
| - | </li>
| |
| - | <li class="HP"><a href="https://2013.igem.org/Team:Tianjin/Human Practice" style="padding:14px 0px 13px 0px;">Human Practice</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Data" style="padding:20px 0px 15px 0px;height:30px;">Data</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Notebook" style="padding:20px 0px 15px 0px;height:30px;">Notebook</a>
| |
| - | </li>
| |
| - | <li><a href=" https://2013.igem.org/Team:Tianjin/Team" style="padding:20px 0px 15px 0px;height:30px;">Team</a>
| |
| - | </li>
| |
| - | <li class="A-C"><a href="https://2013.igem.org/Team:Tianjin/Attributions" style="padding:7px 0px 7px 0px;">Attributions<br/>&<br/>Contributions</a>
| |
| - | </li>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div class="content-section">
| |
| - | </div>
| |
| - |
| |
| - | <div class="logo-section">
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div class="note1">
| |
| - | <style type="text/css">
| |
| - | .box{ width:960px; margin:0 auto; overflow:hidden;}
| |
| - | .main{ width:720px; height:auto; float:right;position:relative;padding:10px 10px 10px 10px;}
| |
| - | .fixed{ width:220px; height:400px;text-align:center;float:left;word-spacing:0.1em;top:10px;margin-top:10px;}
| |
| - | .main img{border:hidden;margin-bottom:5px;}
| |
| - | .main div,li,p{font-family:Arial;line-height:150%;word-spacing:0.1em;}
| |
| - |
| |
| - |
| |
| - | .box p{color:#000;font-family:Arial, Helvetica, sans-serif;font-size:14px;line-height:150%;text-align:left; clear:both;}
| |
| - | .box h1{text-decoration:none;font-weight:normal;color:#0babe7;font-family:Arial;}
| |
| - | .img1{margin:0 150px 15px 150px;padding:5px 5px 5px 5px;background-color:#fafafa;border:thin solid #999; vertical-align:middle;width:400px;}
| |
| - | .img1 a{target="_blank";}
| |
| - |
| |
| - |
| |
| - |
| |
| - | </style>
| |
| - |
| |
| - | <script type="text/javascript">
| |
| - | $(document).ready(function(e) {
| |
| - | t = $('.fixed').offset().top;
| |
| - | mh = $('.main').height();
| |
| - | fh = $('.fixed').height();
| |
| - | $(window).scroll(function(e){
| |
| - | s = $(document).scrollTop();
| |
| - | if(s > t - 100){
| |
| - | $('.fixed').css('position','fixed');
| |
| - | if(s + fh - 6000 > mh){
| |
| - | $('.fixed').css('top',mh-s-fh+'px');
| |
| - | }
| |
| - | }else{
| |
| - | $('.fixed').css('position','');
| |
| - | }
| |
| - | })
| |
| - | });
| |
| - | </script>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div class="box">
| |
| - |
| |
| - | <div class="sub">
| |
| - | <div class="fixed">
| |
| - | <style type="text/css">
| |
| - | li{margin-bottom:0px;}
| |
| - | .cont ul,li{list-style: none;}
| |
| - | .cont ul {padding: 0; margin: 0;text-align:center;}
| |
| - | .cont .hmain{background-color:#0babe7 ;width: 220px;font-size:16px;float: left;border-top:#fff thin solid;border-bottom:#fff thin solid;}
| |
| - |
| |
| - | .cont a {text-decoration: none; /* padding-left: 10px;*/ display: block; display: inline-block; width: 220px;padding-top: 7px;padding-bottom: 7px;}
| |
| - | .cont .hmain a{color:#fff; }
| |
| - | .cont .hmain a:hover{color:#0babe7;background:#fff;border-top:#0babe7 thin solid;border-bottom:#0babe7 thin solid; }
| |
| - | .cont .hmain ul {display: none;}
| |
| - | .cont .hmain li a{font-size:14px;color:#fff;background-color:#8dc7e9;line-height:150%;border:#fff thin solid ;}
| |
| - | .cont .hmain li a:hover{color:#8dc7e9;background:#fff; line-height:150%;border:#8dc7e9 thin solid ;}
| |
| - | </style>
| |
| - |
| |
| - | <script type="text/javascript">
| |
| - | $(document).ready(function(){
| |
| - | $(".hmain").hover(function(){
| |
| - | $(this).children("ul").slideDown();
| |
| - | changeIcon($(this).children("a"));
| |
| - | },function(){
| |
| - | $(this).children("ul").slideUp();
| |
| - | changeIcon($(this).children("a"));
| |
| - | });
| |
| - | });
| |
| - | </script>
| |
| - | <div class="cont">
| |
| - | <ul>
| |
| - | <li class="hmain" style="margin-top:20px;">
| |
| - |
| |
| - | <li class="hmain">
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project">Background</a>
| |
| - | </li>
| |
| - | <li class="hmain">
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Design">Design</a>
| |
| - | </li>
| |
| - | <li class="hmain">
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project">Experiment</a>
| |
| - | <ul>
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment">System construction and optimization</a>
| |
| - | </li>
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Endo-Induce">Endogenous Alkanes Induce</a>
| |
| - | </li>
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Exo-Induce">Exogenous Alkanes Induce</a>
| |
| - | </li>
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/TetA">TetA</a>
| |
| - | </li>
| |
| - | </ul>
| |
| - | </li>
| |
| - |
| |
| - | <li>
| |
| - | <a href="https://2013.igem.org/Main_Page" title="Main Page"><img src="https://static.igem.org/mediawiki/2013/7/75/IGEM-logo-blue.png" width="200px" height="200px" border="none" style="margin-top:80px;"/></a>
| |
| - | </li>
| |
| - | </ul>
| |
| - | </div>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | <div class="main">
| |
| - |
| |
| - | <center><span style="font-family:Arial;font-size:46px;color:#000;"> Experiment</span></center>
| |
| - | </html>
| |
| - |
| |
| - | =1=
| |
| - |
| |
| - |
| |
| - | <html>
| |
| - | <hr/>
| |
| - | <br/>
| |
| - |
| |
| - | <p>Here, we want to test the resistance against tetracycline of Alk-Sensor induced by endogenous alkanes.</p>
| |
| - | <p>We add alkanes outside the cell to test the function of TetA:</p>
| |
| - | <br/>
| |
| - | <div style="text-align:center;vertical-align:middle;"><a href="#" target="_blank" ><img src="#" width="500px" /></a></div>
| |
| - | <br/>
| |
| - | <p><b>Construct:</b></p>
| |
| - | <p>Left: No.60: TetA downstream of Palkm and AlkR downstream of J23103, are cloned into vector PSB1C3 and there’s a alkane producing vector PSB3K3.</p>
| |
| - | <p>Right: No.61: We construct a blank vector PSB3K3 without NPDC and AAR as blank control, and the other vector PSB1C3 is the same as No.60.</p>
| |
| - | <br/>
| |
| - | <p><b>Characterization: </b></p>
| |
| - | <p>Strains of No.60 are cultured in LB medium for about 12 hours. Add 17μg/mL Kan, 12μg/mL Cm and 12μg/mL Tc respectively. After that, inoculate into 3 ml LB medium for an overnight cultures at 37 ℃ with 220rpm shaking.</p>
| |
| - |
| |
| - | <br/>
| |
| - | <div style="text-align:center;vertical-align:middle;"><a href="#" target="_blank" ><img src="#" width="500px" /></a></div>
| |
| - | <br/>
| |
| - |
| |
| - | <p><b>Result:</b> </p>
| |
| - | <p>Alkane producing E.coli can grow in tube but blank vector E.coli cannot grow .</p>
| |
| - | <p><b>Conclusion:</b></p>
| |
| - | <p>Synthesized alkanes induce the expression of alkR, which enables E.coli to survive under the pressure of Tetracycline.</p>
| |
| - | <br/>
| |
| - | <p>Thus, we can get a 3×3 matrix, with promoter strength from weak, medium to strong, and plasmid copy number from small, medium to large.</p>
| |
| - |
| |
| - | <br/>
| |
| - | <div style="text-align:center;vertical-align:middle;"><a href="#" target="_blank" ><img src="#" width="500px" /></a></div>
| |
| - | <br/>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div class="note2">
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <!--
| |
| - | <div class="note">
| |
| - |
| |
| - | </div>
| |
| - | <div class="note">
| |
| - |
| |
| - | </div>
| |
| - | -->
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - | <div id="footer">
| |
| - | <p><br />
| |
| - | <br />
| |
| - | E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
| |
| - | <br />Copyright 2013 © Tianjin University iGEM Team
| |
| - | </p>
| |
| - | </div>
| |
| - |
| |
| - | <!--top button section-->
| |
| - | <div style="display: block" id="goTopBtn">
| |
| - | <a href="#top" title="Top"><img border=0 src="https://static.igem.org/mediawiki/2013/6/61/Top.jpg"></a>
| |
| - | </div>
| |
| - |
| |
| - | </html>
| |
"
