Latest revision as of 20:59, 16 September 2013

07 June 2013

Kristian

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

Colonies of Top 10 E.coli were streaked on two plates
Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

Navigate to the Previous or the Next Entry

@@ Line 1: / Line 1: @@
-{{:Team:DTU-Denmark/Templates/StartPage|09 June 2013}}
+{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}}
-__TOC__
 =lab 208=
@@ Line 9: / Line 8: @@
 *prepare solutions
 *[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
+*plate ''E.coli''
 ==Who was in the lab==
@@ Line 17: / Line 17: @@
 <hr/>
 #1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
-#50% glycerol (500ml)
+#50% glycerol (500 ml)
+#LB medium (liquid) (3 L);
+->20 g of LB in every 1 L of distilled water. Autoclaved medium
+->in 1 of these L, 14 g of agar were added for making LB solid medium
+===plating E.coli===
+*Colonies of Top 10 ''E.coli'' were streaked on two plates
+*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
+Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry
 {{:Team:DTU-Denmark/Templates/EndPage}}