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| - | : '''Phage Purification September - October Notebook: September 1 - September 14 Daily Log'''</font> | + | : '''Phage Purification September - October Notebook: October 14 - October 20 Daily Log'''</font> |
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| - | <font size="4"> '''9/4/13''' </font> | + | <font size="4"> '''10/16/2013''' </font> |
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| - | - Went over the results of our T4 and T7 purification with the Large Phage and small phage group.
| + | Took TEMs of mutated T7 phage for the small phage group. Found 3 small mutants and 1 large. We will repeat this experiment to get more measurements on capsid size. |
| - | - Updated wiki and discussed plans to have everything completed by the deadlines.
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| | + | :-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/10.16T7EM| 10.16 T7 EM]] |
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| - | <font size="4"> '''9/6/13''' </font>
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| - | Continued updating wiki.
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| - | <font size="4"> '''9/7/13''' </font>
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| - | We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.
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| - | :-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient| 9.7 CsCl Gradient]]
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| - | <font size="4"> '''9/9/13''' </font>
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| - | We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
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| - | :-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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| - | <font size="4"> '''9/11/13''' </font>
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| - | - We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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| - | : -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
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| - | <font size="4"> '''9/13/13''' </font>
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| - | - We discussed the results from [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] and determined a new gradient to be run. Our plan is to run three gradients next class, one wild type and two mutant gradients.
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| - | : Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
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| - | : Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6
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| - | : Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6
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| - | - We also worked on our team profile and updated the notebook on the wiki.
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