Template:Kyoto/Notebook/Sep 28

From 2013.igem.org

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!Sample||base pair
!Sample||base pair
|-
|-
-
|9/27 pT181 attenuatorC(C3)1||1174
+
|9/27 pT181 attenuatorC(C3)1||330
|-
|-
-
|9/27 pT181 attenuatorC(C3)2||1174
+
|9/27 pT181 attenuatorC(C3)2||330
|-
|-
-
|9/27 Pcon antisense-DT1||1174
+
|9/27 Pcon antisense-DT1||372
|-
|-
-
|9/27 Pcon antisense-DT2||1174
+
|9/27 Pcon antisense-DT2||372
|}
|}
{| class="wikitable"
{| class="wikitable"
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|94°C||94°C||55°C||68°C||--
|94°C||94°C||55°C||68°C||--
|-
|-
-
|5min||30s||30s||24min||
+
|5min||30s||30s||24s||
|}
|}
 +
</div>
 +
<div class="experiment">
 +
{| class="wikitable"
 +
!Sample||base pair
 +
 +
|-
 +
|9/27 Pcon-RBS-GFP-DT (4&5)||1158
 +
|-
 +
|9/27 Pcon-RBS-GFP-DT (4&5)||1158
 +
|-
 +
|9/27 Pcon-RBS-GFP-DT (4&5)||1158
 +
|-
 +
|9/27 Pcon-RBS-GFP-DT (4&5)||1158
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
 +
|-
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|5min||30s||30s||1min24s||
 +
|}
 +
</div>
 +
===Electrophoresis===
 +
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||Pcon-tetR aptamer-DT+GADPH+A3
 +
|-
 +
|3||Pcon-antisense-Spinach-DT+GADPH+A7
 +
|-
 +
|4||Spinach-DT+GADPH+A1
 +
|-
 +
|5||Pcon-RBS-GFP-DT+GADPH+A5
 +
|-
 +
|6||Pcon-Spinach-DT+GADPH+A9
 +
|}
 +
<br>
 +
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||Pcon-tetR aptamer-DT+gfp+E3
 +
|-
 +
|3||Pcon-antisense-Spinach-DT+gfp+E7
 +
|-
 +
|4||Spinach-DT+gfp+E1
 +
|-
 +
|5||Pcon-RBS-GFP-DT+gfp+E5
 +
|-
 +
|6||Pcon-Spinach-DT+gfp+E9
 +
|}
 +
[[File:Igku_0928_E1.jpg]]<br>
 +
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||Pcon-tetR aptamer-DT+Spinach+C3
 +
|-
 +
|3||Pcon-antisense-Spinach-DT+gfp+C7
 +
|-
 +
|4||Spinach-DT+Spinach+C1
 +
|-
 +
|5||Pcon-RBS-GFP-DT+Spinach+C5
 +
|-
 +
|6||Pcon-Spinach-DT+Spinach+C9
 +
|}
 +
<br>
 +
{| class="wikitable"
 +
!Lane||Sample
 +
|-
 +
|1||100bp ladder
 +
|-
 +
|2||Pcon-tetR aptamer-DT+aptamer+G3
 +
|-
 +
|3||Pcon-antisense-Spinach-DT+aptamer+G7
 +
|-
 +
|4||Spinach-DT+aptamer+G1
 +
|-
 +
|5||Pcon-RBS-GFP-DT+aptamer+G5
 +
|-
 +
|6||Pcon-Spinach-DT+aptamer+G9
 +
|}
 +
[[File:Igku_0928_E2.jpg]]<br>
</div>
</div>

Latest revision as of 03:57, 28 September 2013

Contents

Sep 28

Electrophoresis

Tatsui

LaneSample
1100bp ladder
2Spinach-DT+GAPDH+A1
3Spinach-DT+Spinach+C1
4Spinach-DT+gfp+E1
5Spinach-DT+aptamer+G1
6Pcon-tetR aptamer-DT+GADPH+A3
7Pcon-tetR aptamer-DT+Spinach+C3
8Pcon-tetR aptamer-DT+gfp+E3
9Pcon-tetR aptamer-DT+aptamer+G3
10Pcon-RBS-GFP-DT+GADPH+A5
11Pcon-RBS-GFP-DT+Spinach+C5
12Pcon-RBS-GFP-DT+gfp+E5
13Pcon-RBS-GFP-DT+aptamer+G5
14100bp ladder

File:Igku xxxxxx.xxx

LaneSample
1Pcon-antisense-Spinach-DT+GAPDH+A7
2Pcon-antisense-Spinach-DT+Spinach+C7
3Pcon-antisense-Spinach-DT+gfp+E7
4Pcon-antisense-Spinach-DT+aptamer+G7
5100bp ladder
6Pcon-Spinach-DT+GAPDH+A9
7Pcon-Spinach-DT+Spinach+C9
8Pcon-Spinach-DT+gfp+E9
9Pcon-Spinach-DT+aptamer+G9

File:Igku xxxxxx.xxx

Colony PCR

Samplebase pair
9/27 pT181 attenuatorC(C3)1330
9/27 pT181 attenuatorC(C3)2330
9/27 Pcon antisense-DT1372
9/27 Pcon antisense-DT2372
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s24s
Samplebase pair
9/27 Pcon-RBS-GFP-DT (4&5)1158
9/27 Pcon-RBS-GFP-DT (4&5)1158
9/27 Pcon-RBS-GFP-DT (4&5)1158
9/27 Pcon-RBS-GFP-DT (4&5)1158
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min24s

Electrophoresis

No name

LaneSample
1100bp ladder
2Pcon-tetR aptamer-DT+GADPH+A3
3Pcon-antisense-Spinach-DT+GADPH+A7
4Spinach-DT+GADPH+A1
5Pcon-RBS-GFP-DT+GADPH+A5
6Pcon-Spinach-DT+GADPH+A9


LaneSample
1100bp ladder
2Pcon-tetR aptamer-DT+gfp+E3
3Pcon-antisense-Spinach-DT+gfp+E7
4Spinach-DT+gfp+E1
5Pcon-RBS-GFP-DT+gfp+E5
6Pcon-Spinach-DT+gfp+E9

Igku 0928 E1.jpg

LaneSample
1100bp ladder
2Pcon-tetR aptamer-DT+Spinach+C3
3Pcon-antisense-Spinach-DT+gfp+C7
4Spinach-DT+Spinach+C1
5Pcon-RBS-GFP-DT+Spinach+C5
6Pcon-Spinach-DT+Spinach+C9


LaneSample
1100bp ladder
2Pcon-tetR aptamer-DT+aptamer+G3
3Pcon-antisense-Spinach-DT+aptamer+G7
4Spinach-DT+aptamer+G1
5Pcon-RBS-GFP-DT+aptamer+G5
6Pcon-Spinach-DT+aptamer+G9

Igku 0928 E2.jpg

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