Team:Freiburg/parts/BBa K1150001

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<p id="h1">VP16</p>
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Virus Protein 16 (VP16) is a transcription factor encoded by the UL48 gene of Herpes simplex virus-1 (HSV-1).
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Through complex formation with cellular host factors VP16 can bind to a common regulatory element in the upstream promotor region of immediate-early genes [Weir, 2001]. Through the transactivating function of VP-16 these genes can then be expressed.
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VP16 consists of 490 amino acids with two very important functional domains: a core domain in its central region which is necessary for the indirect DNA binding and a carboxy-terminal transactivation domain (Greaves and O’Hare, 1989; Triezenberg et al., 1988); see Fig.1. The transactivation domain (TAD) of VP16 can be fused to a DNA-binding domain (DBD) of another protein in order to gain expression of a desired target gene [Hirai et al., 2010].
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<tr> <th> VP16 </th> </tr>
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<tr> <td> Function </td> <td> transactivator </td> </tr>
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<tr> <td> Use in </td> <td> Mammalian cells and Herpes simplex virus-1 </td> </tr>
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<tr> <td> RFC standard </td> <td> <a href="http://parts.igem.org/Help:Assembly_standard_25"> RFC 25 </a> </td> </tr>
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<tr> <td> Backbone </td> <td> pSB1C3 </td> </tr>
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<tr> <td> Submitted by </td> <td>
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<a href="https://2013.igem.org/Team:Freiburg"> Freiburg 2013 </a>
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Latest revision as of 23:05, 28 September 2013


VP16

Virus Protein 16 (VP16) is a transcription factor encoded by the UL48 gene of Herpes simplex virus-1 (HSV-1). Through complex formation with cellular host factors VP16 can bind to a common regulatory element in the upstream promotor region of immediate-early genes [Weir, 2001]. Through the transactivating function of VP-16 these genes can then be expressed. VP16 consists of 490 amino acids with two very important functional domains: a core domain in its central region which is necessary for the indirect DNA binding and a carboxy-terminal transactivation domain (Greaves and O’Hare, 1989; Triezenberg et al., 1988); see Fig.1. The transactivation domain (TAD) of VP16 can be fused to a DNA-binding domain (DBD) of another protein in order to gain expression of a desired target gene [Hirai et al., 2010].

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