Team:Groningen/4 July 2013

From 2013.igem.org

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<br/>98 98 85/90 72 72 4  
<br/>98 98 85/90 72 72 4  
<br/>20:00 0:30 0:25 0:27 10:00 forever
<br/>20:00 0:30 0:25 0:27 10:00 forever
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 +
Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
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<table border="1">
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<tr>
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<th>Well</th>
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<th>1</th>
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<th>2</th>
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<th>3</th>
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<th>4</th>
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<th>5</th>
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<th>6</th>
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<th>7</th>
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<th>8</th>
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<th>9</th>
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<th> 10</th>
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</tr>
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<tr>
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<td></td>
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<td>promoter</td>
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<td>Marker</td>
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<td>1</td>
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<td>1.</td>
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<td>2</td>
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<td>2.</td>
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<td>3</td>
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<td>3.</td>
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<td>4</td>
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<td>4.</td>
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</tr>
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</table>
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1. = duplo of sample 1
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 +
Ran the same gel again for 22 min on 100 volt to get a better picture
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<br />(picture was take about an hour and a half after run finished because of our meeting)

Latest revision as of 05:40, 5 July 2013

Sander and Inne


For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever

Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).

Well 1 2 3 4 5 6 7 8 9 10
promoter Marker 1 1. 2 2. 3 3. 4 4.

1. = duplo of sample 1

Ran the same gel again for 22 min on 100 volt to get a better picture
(picture was take about an hour and a half after run finished because of our meeting)

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