Team:MSOE Milwaukee/Primers

From 2013.igem.org

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{{Team:MSOE}}
{{Team:MSOE}}
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<TABLE>
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   <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">GENE</TD>
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   <TD bgcolor = #006331 width = 200 valign = bottom height = 50><font color = "white" size = "+2" >DNA SEQUENCE/ENZYME</TD>
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   <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">ENZYME</TD>
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   <TD bgcolor = #006331 width = 760 valign = bottom height = 50><font color = "white" >PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD>
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  <TD bgcolor = #649327 width = 320 valign = bottom height = 50><font color = "white" size = "+20">SIZE</TD>
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  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">xynC-A</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">PURPOSE</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">594</TD>
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  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">xyloA</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">PURPOSE</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align= left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1020</TD>
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  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaS</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA synthase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1152</TD>
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  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaK1</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">879</TD>
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  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaK2</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate kinase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1011</TD>
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  </TR>
  </TR>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaD</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate-5-pyrophosphate decarboxylase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">954</TD>
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  </TR>
  </TR>
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<TR align = center>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">TPS-CIN</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1800</TD>
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  </TR>
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</TABLE>
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</HTML>

Latest revision as of 21:29, 20 September 2013

  • 1 1
DNA SEQUENCE/ENZYME PRIMER TO ADD THE PREFIX (FOR) 5' to 3'
PRIMER TO ADD THE SUFFIX (REV) 5' to 3'
1,8-cineole synthase ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC
GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG
HMG-CoA Synthase reverse ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG
Mevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG
GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG
Phosphomevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC
Mevalonate Diphosphate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG
GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC
Xylanase C ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC
Xylosidase ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG
GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG

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