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Team:Heidelberg/Templates/Del week10 AG
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==05-08-2013== | ==05-08-2013== | ||
===Amplification from FS_02 to FS_07; 16.4 kb=== | ===Amplification from FS_02 to FS_07; 16.4 kb=== | ||
| - | [[File: | + | [[File:Heidelberg_20130705 DelAG.png|150px|thumb|Gel electrophoresis of DelA-G]] |
| Line 9: | Line 10: | ||
! what !! µl | ! what !! µl | ||
|- | |- | ||
| - | | ''D. acidovorans'' || 1 | + | | ''D. acidovorans'' DSM-39 || 1 |
|- | |- | ||
| FS_02: (1/10) || 1 | | FS_02: (1/10) || 1 | ||
Latest revision as of 12:24, 30 September 2013
05-08-2013
Amplification from FS_02 to FS_07; 16.4 kb
- Reaction
| what | µl |
|---|---|
| D. acidovorans DSM-39 | 1 |
| FS_02: (1/10) | 1 |
| FS_07: (1/10) | 1 |
| Phusion Master Mix | 10 |
| DMSO | 1 |
| dd H2O | 6 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles-PCR | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 6 | 98 | 1 |
| 66 ↓ 0.5 | 5 | |
| 72 | 5min 40s | |
| 24 | 98 | 1 |
| 60 | 5 | |
| 72 | 5min 40s | |
| 1 | 72 | 17 min |
| 1 | 4 | inf |
Results:
- amplification of DelAG did not work
- repeat PCR with higher annealing temperature to increase specifity
