Team:NU Kazakhstan

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<title>NU_Kazakhstan</title>
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!align="center"|[[Team:NU_Kazakhstan|Home]]
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!align="center"|[[Team:NU_Kazakhstan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=NU_Kazakhstan Official Team Profile]
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!align="center"|[[Team:NU_Kazakhstan/Project|Project]]
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!align="center"|[[Team:NU_Kazakhstan/Modeling|Modeling]]
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!align="center"|[[Team:NU_Kazakhstan/Safety|Safety]]
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|<div>Our team consists of five enthusiastic undergraduate students, who manifest genuine interest in the sphere of ''Synthetic Biology''. We became familiar with '''IGEM''' competition in summer 2012 and asked our faculty to organize special Synthetic Biology course in order to learn more about this exciting sphere of biology. Fortunately, our professors also became interested in this competition and were glad to organize two wonderful courses of Synthetic Biology. These courses gave us a great background in many different topics, but the most interesting topic for us was the ''application of aptamers'' in diagnosis, treatment and research. Then, in April 2013 we asked Dr. Damira, our mentor, who has been working with aptamers in our University research center, to join her team in lab.</div> <div>After working with aptamers more closely we started discussing our ideas for the IGEM project. After long discussions we came with the idea of the development of biosensor for detecting cancer at early stages using aptamers. We are planning to construct the system which will be similar to sandwich assay. In this system, aptamers will bind the target protein present in a patient’s serum, and the binding will be detected using aptamers conjugated to quantum dots.</div>
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|align="center"|[[Team:NU_Kazakhstan | Team NU_Kazakhstan]]
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<li><a href="https://2013.igem.org/Team:NU_Kazakhstan">Home</a></li>
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<li><a href="https://2013.igem.org/Team:NU_Kazakhstan/Team">Team</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2013">Official team profile</a></li>
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<li><a href="https://2013.igem.org/Team:NU_Kazakhstan/Modeling">Overview</a></li>
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<li><a href="https://2013.igem.org/wiki/index.php?title=Team:NU_Kazakhstan/E.Coli&action=edit&redlink=1">E. Coli expression system</a></li>
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        <a href="#" rel="1">1</a>
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        <a href="#" rel="2">2</a>
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<div class="pause"><a href="#">II</a></div>
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<div><center><h3>Detection of Carcinoembryonic antigen with sandwich-biosensor
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Diagnosis of certain types of cancer at early stages is still challenging issue. Therefore, many biomarkers for early cancer detection have been investigated. Carcinoembryonic antigen (CEA) is one of the examples of the biomarker which appears at early stages of such types of cancer as colorectal, gastric, pancreatic, lung, and breast carcinoma. In this study, it is planned to develop a biosensor which will be used to detect the presence of CEA. The first part of the study is about selection of ssDNA aptamers, which have strong affinity for CEA, during 12 cycles of SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure, followed by characterization of them using dot-blot analysis, ELONA (Enzyme Linked Oligonucleotide Assay) and SPR (Surface Plasmon Resonance) methods. In the last part, it is planned to clone the genes that will assist in expression of streptavidin on the surface of membrane of the model organisms. The model organisms for creating the biosensor are Escherichia coli and Saccharomyces cerevisiae. E. coli will express the streptavidin through Lpp-Omp expression system, while S. cerevisiae will express this protein through Aga1 – Aga2 system. Since streptavidin has strong affinity to biotin, biotinylated aptamers will be used to make a sandwich biosensor for CEA detection. Twelve cycles of SELEX for CEA have been already finished. </p>
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Latest revision as of 16:06, 27 September 2013

NU_Kazakhstan









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II



Detection of Carcinoembryonic antigen with sandwich-biosensor

Diagnosis of certain types of cancer at early stages is still challenging issue. Therefore, many biomarkers for early cancer detection have been investigated. Carcinoembryonic antigen (CEA) is one of the examples of the biomarker which appears at early stages of such types of cancer as colorectal, gastric, pancreatic, lung, and breast carcinoma. In this study, it is planned to develop a biosensor which will be used to detect the presence of CEA. The first part of the study is about selection of ssDNA aptamers, which have strong affinity for CEA, during 12 cycles of SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure, followed by characterization of them using dot-blot analysis, ELONA (Enzyme Linked Oligonucleotide Assay) and SPR (Surface Plasmon Resonance) methods. In the last part, it is planned to clone the genes that will assist in expression of streptavidin on the surface of membrane of the model organisms. The model organisms for creating the biosensor are Escherichia coli and Saccharomyces cerevisiae. E. coli will express the streptavidin through Lpp-Omp expression system, while S. cerevisiae will express this protein through Aga1 – Aga2 system. Since streptavidin has strong affinity to biotin, biotinylated aptamers will be used to make a sandwich biosensor for CEA detection. Twelve cycles of SELEX for CEA have been already finished.

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