Team:Bonn

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                            <img src="https://static.igem.org/mediawiki/2013/f/f7/IGEM_project_LightProtAct.png" height="170px" >
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                        <div style="width:800px;margin:auto;margin-top:-5px;">
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                            Regulating protein activity is important throughout lifescience research. Even though there are several
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                            systems available for regulation of proteins all suffer from certain disadvantages. </br>
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                            <span style="font-style:italic">Aiming to overcome all of these drawbacks we engineer a novel system for
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                            light dependend regulation of protein degradation resulting in a rapid change of protein activity.
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                            Our system can be not only of great importance for fundamental science to investigate protein function but
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                            also for accurate control of bioreactors and biological machines.</span>
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                        <a href="https://2013.igem.org/Team:Bonn/HP/game/"><img src="https://static.igem.org/mediawiki/2013/f/f8/Bonn_game_titelpic.png" width="950px"></a>
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                                Visit us on Facebook!
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                        <img src="https://static.igem.org/mediawiki/2013/6/67/Bonn_Obiwanecoli001.png">
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                                Project
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                                Making versatile control of biological machines easily possible for everybody we engineer a system for
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                                light dependend control of protein activityper protein degradation.</br>
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                                Using the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused 
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                                protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
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                                activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br>
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                                Modelling of the light dependency of protein degradation will even make simple and accurate control of
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                                protein activity for any desired level of activity possible.</br>
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                                As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br>
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                        <img src="https://static.igem.org/mediawiki/2013/b/b1/Bonn_Masteryoda001.png" height="230px">
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                                To enable protein degradation we decided on one proteases system (ClpXP), which allows specific degradation
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                                of proteins, is highly conserved and well established.
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                                For light dependency we compared several systems to finally find the best suited one. We created a
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                                comprehensive overview of all systems making artificially light control possible so that everyone can
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                                easily choose the optimal system for his approach.
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                                    Human Practice
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                                    The major goal in Human Practices is to make Synthethic Biology easily understandable and intresting for everybody.</br>
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                                    For that purpose we introduce Synthetic Biology and our project to the general public via developing a comic series, programming a computer game and conduct several presentations in schools all over germany.
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                                    Getting the general public even more in touch with science we furthermore planned a Science Slam.
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                Team
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                We are the iGEM team from Bonn consisting of 22 members, studying Biomedicine, Biology, Biotechnology and Mathematics. Organization and planning of our project is carried out by former members, who have already been part of the last year's iGEM Team. Our team is mainly represented by first year bachelor students, making our team highly dynamic, innovative and motivated.
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                We are glad to use parts of the lab of the Life and Medical Sciences Institute in Bonn.
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                We would like to thank all of our sponsors whose support made our project possible.
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            <!-- TODO: Investigate what we are closing here, and if we can do better (if not, DOCUMENT THIS somewhere and make
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                it known to everyone in our Web/PR/Etc. teams.
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                  -- Matthias -->
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{{Template:Team:Bonn:sponsors}}
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Latest revision as of 13:00, 27 October 2013

Retrieved from "http://2013.igem.org/Team:Bonn"