Team:MSOE Milwaukee/Primers

From 2013.igem.org

(Difference between revisions)

Latest revision as of 21:29, 20 September 2013

DNA SEQUENCE/ENZYME	PRIMER TO ADD THE PREFIX (FOR) 5' to 3' PRIMER TO ADD THE SUFFIX (REV) 5' to 3'
1,8-cineole synthase	ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG
HMG-CoA Synthase reverse	ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG
Mevalonate Kinase	ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG
Phosphomevalonate Kinase	ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC
Mevalonate Diphosphate Kinase	ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC
Xylanase C	ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC
Xylosidase	ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG

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 <TABLE>
   <TR align = center>
-   <TD bgcolor = #649327 width = 200 valign = bottom height = 50><font color = "white" >DNA SEQUENCE/ENZYME</TD>
+   <TD bgcolor = #006331 width = 200 valign = bottom height = 50><font color = "white" size = "+2" >DNA SEQUENCE/ENZYME</TD>
-   <TD bgcolor = #649327 width = 760 valign = bottom height = 50><font color = "white">PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD>
+   <TD bgcolor = #006331 width = 760 valign = bottom height = 50><font color = "white" >PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase forward</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase reverse</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" >GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align= left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaS-F</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaS-R</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" >GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaK2</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate kinase</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">mvaD</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate-5-pyrophosphate decarboxylase</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD>
   </TR>
 <TR align = center>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">TPS-CIN</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD>
-   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
+   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD>
   </TR>
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