Template:Team:Uppsala/JS/notebook

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MikaelS (Talk | contribs)

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Latest revision as of 23:26, 4 October 2013 (view source)

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<html>

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var month = ~~new Date().getMonth()~~;

+

var month = 8;

var year = new Date().getFullYear();

var cal_table = '';

var eventString;

var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);

-

var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'

+

var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');

-

,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'

+

var inputs = 106;

-

,'t2013910','t2013911','t2013912','~~t2013913~~','~~t2013914~~','~~t2013915~~','~~t2013919~~');

+

-

var inputs = 96;

+

Date.prototype.getWeek = function()

Line 149:

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eventString = '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

document.getElementById('file_holder').innerHTML = eventString;

+

document.getElementById('calendar').style.marginBottom="350px";

}

Line 186:

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eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';

}

+

document.getElementById('calendar').style.marginBottom="350px";

document.getElementById('file_holder').innerHTML = eventString;

eventString = '';

Line 551:

{

ds = '<div id="dairy-text"><h1>Saturday 2013-08-31</h1><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br>386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1)<br><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation)<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'

+

}

+

else if(id == 'd201391')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-01</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>275. pSBLb4C15-red (dam-)<br>352. pSBLbC3-red<br>406. pSBLbC3-red (dam-)<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>275. pSBLb4C15-red (dam-)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br>406. pSBLbC3-red (dam-)<br><br><b>Screening:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2)<br><br><b>Re-streak:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br><br><h2>Results</h2><br>First screening failed (weird gel)<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201392')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-02</h1><br><br><b>Name of participants:</b> Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br><b>O/N:</b><br>169. pSBLbEcC2-red<br><br><b>Mutagenesis PCR:</b><br>111.7 pSBLb4C15-red *<br>*with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night.<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br>352. pSBLbC3-red*<br>*New attempts at ligation, just in case.<br><br><h2>Results: </h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: No bands<br><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201393')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br>Frozen stock and plasmid preparation:<br>169. pSBLbEcC2-red<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red* <br><i>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</i><br><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br><i>*New attempts at ligation, just in case.</i><br><br><b>Sequencing preparation:</b><br>169. pSBLbEcC2-red<br><br><h2>Characterization experiments:</h2><br><b>Western blot sample preparation on construct:</b><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Results: </h2><br><b>Frozen stock and plasmid preparation:</b><br>169. pSBLbEcC2-red: 44.2 ng/µl<br>169. pSBLbEcC2-red: 47.8 ng/µl<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'

+

}

+

else if(id == 'd201394')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'

+

}

+

else if(id == 'd201395')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work</h2><b>First day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201396')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><b>Second day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'

+

}

+

else if(id == 'd201397')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><b>Third day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'

+

}

+

else if(id == 'd201398')

+

{

+

ds = '<div id="dairy-text"><h1>Sunday 2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: “ <br>414. pSB1C3-CP29-amilCP “<br>415. pSB1C3-CP11-amilCP “<br>417. pSB1C3-CP29-aeBlue “<br>418. pSB1C3-CP11-aeBlue “<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'

+

}

+

else if(id == 'd201399')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1><br><b>Name of participants: </b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Digest</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br> <br><b>Ligation</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Fourth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br></div>'

+

}

+

else if(id == 'd2013910')

+

{

+

ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1><br><b>Name of participants:</b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Re-streak</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br><br><b>Fifth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br></div>'

+

}

+

else if(id == 'd2013911')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1><br><b>Name of participants: </b>Sabri<br><br><h2>Todays Work:</h2><br><b>O/N:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd2013912')

+

{

+

ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1><br><b>Name of participants:</b> Sabri<br><br><h2>Todays Work:</h2><br><b>Plasmid prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br> <br><b>Sequence prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'

+

}

+

else if(id == 'd20131013')

+

{

+

ds ='<h1>European Jamboree Lyon end</h1>';

+

}

+

else if(id == 'd20131012')

+

{

+

ds ='<h1>European Jamboree Lyon</h1>';

+

}

+

else if(id == 'd20131011')

+

{

+

ds ='<h1>European Jamboree Lyon start</h1>';

+

}

+

else if(id == 'd2013104')

+

{

+

ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';

+

}

+

else if(id == 'd2013102')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb47. reuteri 100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Sequencing preparation:</b><br>Lb47. reuteri 100-23 pSBLbE-red<br></div>'

+

}

+

else if(id == 'd2013101')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs </h2><br>Lb47. reuteri 100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Screening PCR:</b><br>Lb47. reuteri 100-23 pSBLbE-red<br><br><h2>Results</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2: <b>Growing</b><br>Lb13. pJP059: <b>Growing</b><br>420. pSBLbE-CP11-amilCP: Not growing<br>435. pSBLbC-CP29-amilCP: Not growing<br>440. pSBLbC-red: Not growing<br>441. pSBLbE-red: <b>Growing</b><br>All negative controls were clear, and the positive controls grew</b>.<br></div>'

+

}

+

else if(id == 'd2013928')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1><br><b>Name of participants:</b> Viktor Blomkvist, Mikael Strandgren, Erik Edlund<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><b>L. reuteri:</b><br>Lb3. pVS2<br><br><b>Lc. lactis:</b><br>Lb13. pJP059<br><br><h2>Todays work</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2<br>Lb13. pJP059<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'

+

}

+

else if(id == 'd2013927')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Overnight:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br><br><b>Streak from frozen stock (for photos):</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'

+

}

+

else if(id == 'd2013924')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br><br><h2>Results</h2><br><b>Transformation</b>:<br>No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday.<br></div>'

+

}

+

else if(id == 'd2013921')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist <br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Transformation</b><br><i>To L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2 (positive control)<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>Erythromycin<br>Chloramphenicol <br>Antibiotic-free<br></div>'

+

}

+

else if(id == 'd2013920')

+

{

+

ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'

}

else if(id == 'd2013919')

Line 556:

Line 648:

ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';

}

+

else if(id == 'd2013918')

+

{

+

ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1><br><b>Name of participants: </b> Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>O/N:</b><br>435. pSBLbC-CP29-amilCP<br></div>'

+

}

+

else if(id == 'd2013917')

+

{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>435. pSBLbC-CP29-amilCP<br></div>'

+

}

+

else if(id == 'd2013916')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>440. pSBLbC-red<br><br><b>Assembly and transformation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'

+

}

else

{

@@ Line 1: / Line 1: @@
 <html>
 <script type="text/javascript">
-var month = new Date().getMonth();
+var month = 8;
 var year = new Date().getFullYear();
 var cal_table = '';
 var eventString;
 var days_in_month = new Array(31,28,31,30,31,30,31,31,30,31,30,31);
-var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385'
+var days_with_dirys = new Array('t2013610','t2013612','t2013613','t2013617','t2013618','t2013619','t2013620','t2013621','t2013622','t2013623','t2013624','t2013625','t2013626','t2013627','t2013628','t2013629','t2013630','t2013630','t201371','t201372','t201373','t201374','t201375','t201376','t201377','t201379','t201378','t201379','t2013710','t2013711','t2013712','t2013713','t2013714','t2013715','t2013716','t2013717','t2013718','t2013719','t2013720','t2013721','t2013722','t2013723','t2013724','t2013725','t2013726','t2013727','t2013728','t2013729','t2013730','t2013731','t201381','t201382','t201383','t201384','t201385','t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399','t2013910','t2013911','t2013912','t2013917','t2013918','t2013919','t2013920','t2013921','t2013924','t2013927','t2013928','t2013101','t2013102','t2013104','t20131011','t20131012','t20131013');
-,'t201386','t201387','t201388','t201389','t2013810','t2013811','t2013812','t2013813','t2013814','t2013815','t2013816','t2013817','t2013818','t2013819','t2013820','t2013821','t2013822','t2013823','t2013824','t2013825','t2013826','t2013827','t2013828','t2013829','t2013830','t2013831','t201391','t201392','t201393','t201394','t201395','t201396','t201397','t201398','t201399'
+var inputs = 106;
-,'t2013910','t2013911','t2013912','t2013913','t2013914','t2013915','t2013919');
-var inputs = 96;
 Date.prototype.getWeek = function()
@@ Line 149: / Line 147: @@
    eventString =  '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
    document.getElementById('file_holder').innerHTML = eventString;
+document.getElementById('calendar').style.marginBottom="350px";
   }
@@ Line 186: / Line 185: @@
      eventString = eventString + '<div class="eventBox" id="'+'d'+y+m+day+'" onclick="diry('+y+', '+m+', '+day+');"><h1>'+y+'-'+m+'-'+day+'</h1></div>';
     }
+document.getElementById('calendar').style.marginBottom="350px";
    document.getElementById('file_holder').innerHTML = eventString;
    eventString = '';
@@ Line 551: / Line 551: @@
   {
 	ds = '<div id="dairy-text"><h1>Saturday  2013-08-31</h1><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br>386. pSBLbEcC2-red (in PK401 dam-) (pEL+replicon from pAMbeta1)<br><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation)<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>169. pSBLbEcC2-red (ligation): No band, possibly to weak due to being ligation → transform and screen.</div>'
+ }
+      else if(id == 'd201391')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-01</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Alona Nyberg, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red<br>406. pSBLbC3-red (dam-)<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL+ replicon from pJP)<br>275.  pSBLb4C15-red (dam-)<br>352. pSBLbC3-red (pEL+ replicon from pVS2)<br>406. pSBLbC3-red (dam-)<br><br><b>Screening:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1) (x2)<br><br><b>Re-streak:</b><br>169. pSBLbEcC2-red (pEL+replicon from pAMbeta1)<br><br><h2>Results</h2><br>First screening failed (weird gel)<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201392')
+ {
+	ds = '<div id="dairy-text"><h1>Monday  2013-09-02</h1><br><br><b>Name of participants:</b> Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br><b>O/N:</b><br>169. pSBLbEcC2-red<br><br><b>Mutagenesis PCR:</b><br>111.7 pSBLb4C15-red *<br>*with primers to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried). Two attempts, second over night.<br><br><b>Ligation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br>352. pSBLbC3-red*<br>*New attempts at ligation, just in case.<br><br><h2>Results: </h2><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: No bands<br><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>failed.<br><br><h2>Characterization experiments:</h2><br><b>Western blot on construct:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201393')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-03</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>261. pSB3K3-J23110-B0034-His-4CL<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work: </h2><br>Frozen stock and plasmid preparation:<br>169. pSBLbEcC2-red<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red* <br><i>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</i><br><br><b>Transformation:</b><br>111. pSBLb4C15-red (pEL4C15+replicon from pJP059)*<br><i>*New attempts at ligation, just in case.</i><br><br><b>Sequencing preparation:</b><br>169. pSBLbEcC2-red<br><br><h2>Characterization experiments:</h2><br><b>Western blot sample preparation on construct:</b><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Results: </h2><br><b>Frozen stock and plasmid preparation:</b><br>169. pSBLbEcC2-red: 44.2 ng/µl<br>169. pSBLbEcC2-red: 47.8 ng/µl<br><br><b>Gel electrophoresis:</b><br>111.7 pSBLb4C15-red: Correct bands in five lanes of six, weird ladder.</div>'
+ }
+      else if(id == 'd201394')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-04</h1><br><br><b>Name of participants: </b>Anders Edlund, Mikael Strandgren, Stephanie Herman, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs: </h2><br>111. pSBLb4C15-red<br>169. pSBLbEcC2-red<br>352. pSBLbC3-red<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>Assembly:</b><br>353. pSBLb4E15-red (ermR-cassette from three different sources, 2.1, 2.3 and old)<br><br><b>Trasformation:</b><br>111.7 (old, used in earlier attempts of ermR, cut with SacI+SalI and ligated to test RFP)<br>353. pSBLb4E15-red <br><br><b>PCR-purification:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)<br><br><b>DpnI-treatment:</b><br>111.7 pSBLb4C15-red* <br>*to change the in the chloramphenicol-cassette promotor into one from the CP-serie. (CP11, CP29 and CP30 were tried)</div>'
+ }
+      else if(id == 'd201395')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-05</h1><br><b>Name of participants:</b> Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs:</h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work</h2><b>First day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201396')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-06</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work: </h2><br><b>PCR-purification and recircularization:</b><br>Mutagenesis´d pSBLb4C15 with promotor in CmR changed to CP11, CP29 or CP30)<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red*<br><i>*to do a new plasmid preparation since old may have corrupt red-cassette/contamination)</i><br><br><b>Streak from frozen stock:</b><br>111.7 pSBLb4C15-red*<br><i>* to test if red-cassette has been damaged, or possibly the stock contaminated.</i><br><br><b>Second day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Characterization:</h2><br><b>Western blot on construct: </b><br>335. pSB3K3-J23101-HIs-STS</div>'
+ }
+      else if(id == 'd201397')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-07</h1><br><br><b>Name of participants:</b> Mikael Strandgren, Christoffer L<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work:</h2> <br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE) <br>355.2 pSB3K3-CP29-mCherry<br>355.3 pSB3K3-CP29-mCherry<br>357.3 pSB3K3-CP30-usp45-mCherry<br>357.4 pSB3K3-CP30-usp45-mCherry<br>358.1 pSB3K3-CP29-usp45-mCherry<br>358.2 pSB3K3-CP29-usp45-mCherry<br>363. pSB3K3-CP30-tspurple<br>364. pSB3K3-CP29-tspurple<br>365. pSB3K3-CP11-tspurple<br>366. pSB3K3-CP30-amilCP<br>367. pSB3K3-CP29-amilCP<br>368. pSB3K3- CP11-amilCP<br>369. pSB3K3-CP30-aeblue<br>370. pSB3K3-CP29-aeblue<br>371. pSB3K3-CP11-aeblue<br><br><b>Assembly:</b><br>407. pSB1C3-CP29-Mcherry*<br>408. pSB1C3-CP30-usp45-mCherry*<br>409. pSB1C3-CP29-usp45-mCherry*<br>410. pSB1C3-CP30-tsPurple*<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP* <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue*<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red (two variants, from two different sources of ermR. 419.1=2.3, 419.2=old)<br>* pSB1C3 ran out after six ligations. New was made and ligated later, but couldn’t be included in the transformation today.<br><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>417. pSB1C3-CP29-aeBlue <br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red <br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette.<br><br><b>O/N:</b><br>111.7 pSBLb4C15-red<br><br><b>Third day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br><h2>Results: </h2><br><b>O/N:</b><br>111.7 pSBLb4C15-red: Little growth, since 15ml-tube had to be used, and the lid was closed to tight. → new ON today.<br><br><b>Plasmid preparation:</b><br>351.5 pSB1C3-pemp-mut1 (mutE): 205.6 ng/µl<br>355.2 pSB3K3-CP29-mCherry: 90.6 ng/µl<br>355.3 pSB3K3-CP29-mCherry: 79.3 ng/µl<br>357.3 pSB3K3-CP30-usp45-mCherry: 77.3 ng/µl<br>357.4 pSB3K3-CP30-usp45-mCherry: 95.5 ng/µl<br>358.1 pSB3K3-CP29-usp45-mCherry: 90.4 ng/µl<br>358.2 pSB3K3-CP29-usp45-mCherry: 87.1 ng/µl<br>363. pSB3K3-CP30-tspurple: 75.5 ng/µl<br>364. pSB3K3-CP29-tspurple: 43.9 ng/µl<br>365. pSB3K3-CP11-tspurple: 47.3 ng/µl<br>366. pSB3K3-CP30-amilCP: 74.5 ng/µl<br>367. pSB3K3-CP29-amilCP: 77.2 ng/µl<br>368. pSB3K3- CP11-amilCP: 34.3 ng/µl<br>369. pSB3K3-CP30-aeblue: 37.4 ng/µl<br>370. pSB3K3-CP29-aeblue: 60.3 ng/µl<br>371. pSB3K3-CP11-aeblue: 76.2 ng/µl </div>'
+ }
+      else if(id == 'd201398')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday  2013-09-08</h1><br><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs: </h2><br>407. pSB1C3-CP29-Mcherry<br>408. pSB1C3-CP30-usp45-mCherry<br>409. pSB1C3-CP29-usp45-mCherry<br>410. pSB1C3-CP30-tsPurple<br>411. pSB1C3-CP29-tsPurple <br>412. pSB1C3-CP11-tsPurple <br>413. pSB1C3-CP30-amilCP <br>414. pSB1C3-CP29-amilCP <br>415. pSB1C3-CP11-amilCP <br>416. pSB1C3-CP30-aeBlue<br>417. pSB1C3-CP29-aeBlue<br>418. pSB1C3-CP11-aeBlue<br>419. pSBLbEcE-red<br><br><h2>Todays work: </h2><br><b>Transformations:</b><br>411. pSB1C3-CP29-tsPurple *<br>412. pSB1C3-CP11-tsPurple *<br>414. pSB1C3-CP29-amilCP *<br>415. pSB1C3-CP11-amilCP *<br>417. pSB1C3-CP29-aeBlue *<br>418. pSB1C3-CP11-aeBlue *<br>* The ones yesterday were by mistake spread on Kanamycin-plates, the same ones are now spread on chloramphenicol with 50x-dilutions, hoping they are still good.<br><br><b>ON:</b><br>353. pSBLB4E15-red<br><br><h2>Results: </h2><br><b>Transformation</b><br>411. pSB1C3-CP29-tsPurple: Growth, but spread on kanamycin plates by mistake (source of cp:s was pSB3K3) → Spread on C-plates today.<br>412. pSB1C3-CP11-tsPurple: 			“ <br>414. pSB1C3-CP29-amilCP 			“<br>415. pSB1C3-CP11-amilCP 			“<br>417. pSB1C3-CP29-aeBlue 			“<br>418. pSB1C3-CP11-aeBlue			“<br>419. pSBLbEcE-red: Growth covering the plates, only white colonies.<br>pSBLb4C15-red with CP11, CP29 or CP30 in cmR-cassette: No growth</div>'
+ }
+      else if(id == 'd201399')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-09</h1><br><b>Name of participants: </b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Digest</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br> <br><b>Ligation</b><br>338.pSB1C3-meffBlue<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Fourth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br><br></div>'
+ }
+      else if(id == 'd2013910')
+ {
+	ds = '<div id="dairy-text"><h1>Tueday 2013-09-10</h1><br><b>Name of participants:</b> Sabri, Christoffer L<br><br><h2>Todays Work:</h2><br><b>Re-streak</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br><br><b>Fifth day of survival test:</b><br>O/N in antibiotic-free medium and re-streak on plates with and without antibiotics.<br></div>'
+ }
+      else if(id == 'd2013911')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-11</h1><br><b>Name of participants: </b>Sabri<br><br><h2>Todays Work:</h2><br><b>O/N:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+      else if(id == 'd2013912')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-09-12</h1><br><b>Name of participants:</b> Sabri<br><br><h2>Todays Work:</h2><br><b>Plasmid prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue<br> <br><b>Sequence prep:</b><br>339. pSB1C3-gfasPurple<br>340. pSB1C3-AmajCP(AmajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>338.pSB1C3-meffBlue</div>'
+ }
+ else if(id == 'd20131013')
+ {
+  ds ='<h1>European Jamboree Lyon end</h1>';
+ }
+ else if(id == 'd20131012')
+ {
+  ds ='<h1>European Jamboree Lyon</h1>';
+ }
+ else if(id == 'd20131011')
+ {
+  ds ='<h1>European Jamboree Lyon start</h1>';
+ }
+ else if(id == 'd2013104')
+ {
+  ds ='<h1>WIKIFREEZE 23.59 Boston time</h1>';
+ }
+      else if(id == 'd2013102')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-10-02</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Sequencing preparation:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br></div>'
+ }
+       else if(id == 'd2013101')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-10-01</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs </h2><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Todays work</h2><br><b>Screening PCR:</b><br>Lb47. reuteri  100-23 pSBLbE-red<br><br><h2>Results</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2: <b>Growing</b><br>Lb13. pJP059: <b>Growing</b><br>420. pSBLbE-CP11-amilCP: Not growing<br>435. pSBLbC-CP29-amilCP: Not growing<br>440. pSBLbC-red: Not growing<br>441. pSBLbE-red: <b>Growing</b><br>All negative controls were clear, and the positive controls grew</b>.<br></div>'
+ }
+       else if(id == 'd2013928')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-28</h1><br><b>Name of participants:</b> Viktor Blomkvist, Mikael Strandgren, Erik Edlund<br><br><h2>Ongoing constructs</h2><br><b>E. coli:</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><b>L. reuteri:</b><br>Lb3. pVS2<br><br><b>Lc. lactis:</b><br>Lb13. pJP059<br><br><h2>Todays work</h2><br><b>Transformation:</b><br><i>To L. reuteri 100-23, L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2<br>Lb13. pJP059<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013927')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-27</h1><br><b>Name of participants: </b>Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Overnight:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb11. Plantarum 256 no plasmid<br>Lb12. Reuteri DSM20016 no plasmid<br><br><b>Streak from frozen stock (for photos):</b><br>420. pSBLbE-CP11-amilCP<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br></div>'
+ }
+       else if(id == 'd2013924')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-24</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br><br><h2>Results</h2><br><b>Transformation</b>:<br>No growth. Failed negative controls and bad electroporations encourage us to try again when we have time, probably next saturday.<br></div>'
+ }
+       else if(id == 'd2013921')
+ {
+	ds = '<div id="dairy-text"><h1>Saturday 2013-09-21</h1><br><b>Name of participants: </b>Erik Edlund, Mikael Strandgren, Viktor Blomkvist <br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Todays work</h2><br><b>Transformation</b><br><i>To L. reuteri DSM 20016 and L. plantarum 256:</i><br>Lb3. pVS2 (positive control)<br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br>441. pSBLbE-red <br><br><h2>Other experiments</h2><br><b>Making MRS-agar plates:</b><br>Erythromycin<br>Chloramphenicol <br>Antibiotic-free<br></div>'
+ }
+       else if(id == 'd2013920')
+ {
+	ds = '<div id="dairy-text"><h1>Friday 2013-09-20</h1><br><b>Name of participants: </b>Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
   }
   else if(id == 'd2013919')
@@ Line 556: / Line 648: @@
    ds ='<h1>Uppsala iGEMs Synbioday, 12.15 to 17.00</h1>';
   }
+       else if(id == 'd2013918')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-09-18</h1><br><b>Name of participants: </b> Viktor Blomkvist<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>O/N:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+        else if(id == 'd2013917')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday 2013-09-17</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
+         else if(id == 'd2013916')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-09-16</h1><br><b>Name of participants: </b> Mikael Strandgren, Anders Edlund<br><br><h2>Ongoing constructs</h2><br>435. pSBLbC-CP29-amilCP<br>440. pSBLbC-red<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>440. pSBLbC-red<br><br><b>Assembly and transformation:</b><br>435. pSBLbC-CP29-amilCP<br></div>'
+ }
   else
   {

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Latest revision as of 23:26, 4 October 2013