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| | <h2 class="title2">Characterizations</h2> | | <h2 class="title2">Characterizations</h2> |
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| - | <h3 class="title3">XOR1</h3>
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| | + | <h3 class="texteright"><span class="title3">Riboswitches</span></h3> |
| | + | <h3 class="texteleft"><span class="title3">Logic Gates</span></h3> |
| | + | <div class="clear"></div> |
| | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/ribo"><img src="https://static.igem.org/mediawiki/2013/b/b3/Ribo_-_340px.png" class="imgcontentright" /></a> |
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| - | <div class="accordeon">
| + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/ results/ logic_gates"><img src="https://static.igem.org/mediawiki/2013/2/ 26/ Gates. jpg" class=" imgcontentleft" /></a> |
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2> | + | |
| - | <div class="infos">
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| - | <img style="width:340px;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
| + | |
| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/ notebook/ protocols/charac_recomb"> In vitro recombinase characterization protocol</a></p> | + | |
| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p> | + | |
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| - | </div>
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| - | <div class="accordeon">
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| | | | |
| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| - | <br>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | </div>
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| | <div class="clear"></div> | | <div class="clear"></div> |
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| - | <h3 class="title3">AND1</h3>
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| - | <div class="accordeon">
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - |
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | </div>
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| - | <div class="accordeon">
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| - |
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
| + | <br> |
| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <br>
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| - | </div>
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| - | </div>
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| | + | <h3 class="texteleft"><span class="title3">Carry Diffusion Model</span></h3> |
| | + | <h3 class="texteright"><span class="title3">T7 Polymerase</span></h3> |
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| - | <div class="clear"></div> | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/carry"><img src="https://static.igem.org/mediawiki/2013/1/1b/Diffusion_results340px.png" class="imgcontentleft" /></a> |
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| - | | + | < a href="https:// 2013.igem.org/ Team:INSA_Toulouse/ contenu/ lab_practice/ results/ polT7"><img style="width:340px;" src="https://static.igem.org/mediawiki/2013/ 1/ 14/ Pol_t7.jpg" class=" imgcontentright" /> </a> |
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| - | <h3 class="title3">Red Light Sensor</h3>
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| - | | + | |
| - | <div class="accordeon">
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| - | | + | |
| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2> | + | |
| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px ; float:right;" src="https://static.igem.org/mediawiki/2013/ 6/ 6b/ XOR1_bio_80.jpg" class=" imgcontent" /> | + | |
| | <div class="clear"></div> | | <div class="clear"></div> |
| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - |
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | </div>
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| - | <div class="accordeon">
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| - |
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
| + | <br> |
| - | <br>
| + | |
| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <br>
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| - | </div>
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| - | </div>
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| | + | <h3 class="texteleft"><span class="title3">Blue Light Sensor</span></h3> |
| | + | <h3 class="texteright"><span class="title3">Red Light Sensor</span></h3> |
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| - | <h3 class="title3">Blue Light Sensor</h3>
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| | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/blue_sensor"><img style="width:340px;" src="https://static.igem.org/mediawiki/2013/f/f1/Blue_light_system_notebook_-_340px.png" class="imgcontentleft" /></a> |
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| | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/red_sensor"> <img src="https://static.igem.org/mediawiki/2013/5/5b/Red_light_système_-_340px.png" class="imgcontentright" /></a> |
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| - | <div class="accordeon">
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| | <div class="clear"></div> | | <div class="clear"></div> |
| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | </div>
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| - | <div class="accordeon">
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| | <br> | | <br> |
| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
| + | <br> |
| - | <br>
| + | <h3 class="texteleft"><span class="title3">General Inducer</span></h3> |
| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p> | + | |
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| - | </div>
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| - | </div>
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| | + | <div class="clear"></div> |
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| - | <h3 class="title3">Pol T7</h3> | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/general_inducer"><img src="https://static.igem.org/mediawiki/2013/9/9e/General_inducer_notebook_-_340px.png" class="imgcontentleft" /></a> |
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| - | <div class="accordeon">
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2> | + | |
| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| | <div class="clear"></div> | | <div class="clear"></div> |
| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| - | <br>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | <div class="accordeon">
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/7/72/Test_carac.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <div class="clear"></div>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <br>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <br>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | </div>
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| - | <h3 class="title3">General inductor</h3>
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| - | <div class="accordeon">
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| - |
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <div class="infos">
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| - | <img src="https://static.igem.org/mediawiki/2013/d/d4/General_inducer_notebook_-_680px.png" class="imgcontent" />
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| - | <p class="texte">The couple TetR-pTet system construction is a first step to characterize the entire system. A constitutive promoter (BBa_J23116) was assemble to tetR and then assemble to pTet-rbs-RFP-term (Bba_I13521). pTet is a regulator constitutively ON that expresses the mRFP1 protein. TetR is an inducer that binds to pTet promoter and thus, represses expression the downstream system. Supply of tetracycline or its analog aTc (anhydrotetracycline) is known to bind to tetR and invert the operation (inhibits expression of mRFP in this case).<br>
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| - | After 18 hours, clones containing this assembly (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response. <br>
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| - | Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL).</p>
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