Team:DTU-Denmark

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Our project simultaneously cleans and reclaims energy from waste water streams by using two E. coli mutants to turn ammonia into nitrous oxide.
 
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[[File:DTU Project sketch v2.png|500px]]
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Industrial processes have greatly increased the amount of fixed nitrogen, leading to an excess of ammonia in the environment.
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Ammonia is a common pollutant in agricultural runoff, and excess in the environment leads to increased algae growth (eutrophication) that potentially starves other water-borne organisms for oxygen.  
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Our project reverses nitrogen fixation, and is a means to clean up polluted streams and wastewater.
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The final product is nitrous oxide, which can be reclaimed and used as a fuel source.
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In effect, we are turning pollution into energy.
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==Highlights==
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* We successfully [[Team:DTU-Denmark/Experiment4|characterized ammonia consumption of our AMO transformant (Mutant 1)]].
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* We constructed and characterized a [[Team:DTU-Denmark/pBAD_SPL|synthetic promoter library]] built on the arabinose inducible promoter.
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* We successfully [[Team:DTU-Denmark/HelloWorld|targeted proteins to the periplasm]] in our Hello World pilot project.
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* We [[Team:DTU-Denmark/Modeling|modeled]] several aspects of the project including [[Team:DTU-Denmark/Kinetic_Model|the kinetics of the system]] and [[Team:DTU-Denmark/Reactor_Model|the size of reactor]] required.
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* We conducted several events around [[Team:DTU-Denmark/Human_Practices|human practices]], including a [[Team:DTU-Denmark/Biobrick_Workshop|biobrick workshop]] and a [[Team:DTU-Denmark/IP_and_Synthetic_Biology|discussion on IP and synthetic biology]].
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For more information on our project, please see [[Team:DTU-Denmark/Project| our project details]]. Also be sure to check out our [[Team:DTU-Denmark/Notebook| online lab notebook]].
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Latest revision as of 20:58, 4 October 2013

Highlights

For more information on our project, please see our project details. Also be sure to check out our online lab notebook.

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