Team:UNITN-Trento/Notebook/Labposts/07/40

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(Difference between revisions)

Latest revision as of 10:41, 3 October 2013

{"date" : "2013-07-18","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : " today we performed the miniprep protocol on our inocula. yealds are:
006 a: 169,3 ng/ul;
006 b: 204,4ng/ul;
016 a: 204,9 ng/ul;
016 b: 173,7 ng/ul;
030 a: 276ng/ul;
030 b: 375,8ng/ul;
082 a: 173,1ng/ul;
082 b: 225,9 ng/ul;
After that we screened 006b, 016a,082 and 030.

gel 1

gel 2

As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}

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+{"date" : "2013-07-18","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : "<html>  today we performed the miniprep protocol on our inocula. yealds are: <br>006 a: 169,3 ng/ul; <br>006 b: 204,4ng/ul; <br>016 a: 204,9 ng/ul; <br>016 b: 173,7 ng/ul; <br>030 a: 276ng/ul; <br>030 b: 375,8ng/ul; <br>082 a: 173,1ng/ul; <br>082 b: 225,9 ng/ul; <br>After that we screened 006b, 016a,082 and 030.<br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 1|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3f/Tn-2013_a.jpg\" width=\"450px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 2|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/18/Tn.2013_B.jpg\" width=\"450px\" /></center></html>}}<html>As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}
-	"date" : "2013-07-20",
-	"author" : "fabio-bruno",
-	"title" : " blue and red light sensors!!",
-	"content" : "<html> this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula. <br>Second set yields are: <br>006a : 289 <br>006b : 409.3<br>016° : 188.8<br>016b :266.6<br>030a : 388.6<br>030b : 270<br>082a : 433.0<br>082b : 568<br>Third set yiealds are: <br>006: 321 ng/ul<br>016: 440.9 ng/ul<br>R0082: 441,5 ng/ul<br>030: 199,9 ng/ul<br>These ones haven’t been screened so far. <br>Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.</html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
-}