Team:DTU-Denmark

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== Requiem for a Stream:<br>From Ammonia Pollution to Energy Production via Denitrification ==
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Intense agriculture is the base of feeding a growing world population. However it comes at a cost for the environment. As the demand for food articles is steadily increasing farmers turn to extensive use of fertilizers and nitrogen-enriched animal feeds to increase their yield. Much of this nitrogen comes in the form of ammonia and accumulates in the argicultural runoff, which pollutes streams and the groundwater.
 
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Our project removes ammonia from waste water, and via two ''E. coli'' mutants turns it into nitrous oxide.
 
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[[File:Dtu overview.png|400px|center]]
 
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Ammonia is a common pollutant in agricultural runoff, and excess ammonia in the environment leads to increased algae growth and eutrophication, which starves other water-borne organisms for oxygen. Our project reverses nitrogen fixation, and is a means to clean up polluted streams and wastewater.  The final product is nitrous oxide, which can be sold as an industrial product or reclaimed and used as a source of small scale sustainable energy.
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For more information on the mutants, please see [[Team:DTU-Denmark/Project| project details]], and for information on the experiments we did to characterize the mutants, see [[Team:DTU-Denmark/Experiments|an overview of the experiments]] we conducted.  Also be sure to check out our [[Team:DTU-Denmark/Notebook| online lab notebook]], and the [[Team:DTU-Denmark/Human_Practices | human practices events]] that we hosted.
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==Highlights==
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* We successfully [[Team:DTU-Denmark/Experiment4|characterized ammonia consumption of our AMO transformant (Mutant 1)]].
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* We constructed and characterized a [[Team:DTU-Denmark/pBAD_SPL|synthetic promoter library]] built on the arabinose inducible promoter.
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* We successfully [[Team:DTU-Denmark/HelloWorld|targeted proteins to the periplasm]] in our Hello World pilot project.
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* We [[Team:DTU-Denmark/Modeling|modeled]] several aspects of the project including [[Team:DTU-Denmark/Kinetic_Model|the kinetics of the system]] and [[Team:DTU-Denmark/Reactor_Model|the size of reactor]] required.
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* We conducted several events around [[Team:DTU-Denmark/Human_Practices|human practices]], including a [[Team:DTU-Denmark/Biobrick_Workshop|biobrick workshop]] and a [[Team:DTU-Denmark/IP_and_Synthetic_Biology|discussion on IP and synthetic biology]].
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For more information on our project, please see [[Team:DTU-Denmark/Project| our project details]].  Also be sure to check out our [[Team:DTU-Denmark/Notebook| online lab notebook]].
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Latest revision as of 20:58, 4 October 2013

Highlights

For more information on our project, please see our project details. Also be sure to check out our online lab notebook.