Team:UNITN-Trento/Notebook/Labposts/07/50

From 2013.igem.org

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{"date" : "2013-07-22","author" : "thomas","title" : "Inocula of AraC-pBAD + EFE + Venus","content" : "<html>This is the part four of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37&deg;C in thermoshaker.  </html>","tags" : "EFE-Venus"}
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"date" : "2013-07-23",
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"author" : "bruno",
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"title" : "Blue sensor",
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"content" : "<html>I made mini prep from inoculum overnight. I oubtained the following yields:<br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>K952003-1 </td><td>1019,1 ng/ul</td></tr><tr><td>K952003-2</td><td>1715,7 ng/ul</td></tr><tr><td>K952003-3 </td><td>180,0  ng/ul</td></tr><tr><td>K952003-4</td><td>236,4 ng/ul</td></tr></TABLE></center></html>}}<html><br/>from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.</html>",
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"tags" : "BBa_K952003"
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}
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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-22","author" : "thomas","title" : "Inocula of AraC-pBAD + EFE + Venus","content" : "This is the part four of this experiment. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37°C in thermoshaker. ","tags" : "EFE-Venus"}