Team:UNITN-Trento/Notebook/Labposts/07/55

From 2013.igem.org

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{"date" : "2013-07-23","author" : "bruno","title" : "Blue sensor","content" : "<html>I made mini prep from inoculum overnight. I oubtained the following yields:<br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>K952003-1 </td><td>1019,1 ng/ul</td></tr><tr><td>K952003-2</td><td>1715,7 ng/ul</td></tr><tr><td>K952003-3 </td><td>180,0  ng/ul</td></tr><tr><td>K952003-4</td><td>236,4 ng/ul</td></tr></TABLE></center></html>}}<html><br/>from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.</html>","tags" : "BBa_K952003"}
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"date" : "2013-07-25",
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"author" : "fabio",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html> today I miniprepped and screened (on a medium and a large size gel) the 6 ligation inocula! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/c/ce/Tn-2013Eee.jpg\" width=\"450px\" /></center></html>}}<html><br>As we can see from the gel the ligation seems not to be occurred!! I forgot to run the plasmid (016) alone to see a difference with the ligations though!! However I need to digest k592016 again to repeat another ligation with the blu promoter!!this time I digested 3 ug of the A sample from the first set!!</html>",
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"tags" : " YF1_FixJ - FixK2"
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Latest revision as of 10:45, 3 October 2013

{"date" : "2013-07-23","author" : "bruno","title" : "Blue sensor","content" : "I made mini prep from inoculum overnight. I oubtained the following yields:

Quantification Results
SampleConcentration
K952003-1 1019,1 ng/ul
K952003-21715,7 ng/ul
K952003-3 180,0 ng/ul
K952003-4236,4 ng/ul

from that I made an overnight digestion of 5ug of DNA with EcoRI and XbaI but only for 003-1 sample, two set of digestion.","tags" : "BBa_K952003"}