Team:UNITN-Trento/Notebook/Labposts/07/61

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:46, 3 October 2013

{"date" : "2013-07-26","author" : "viola","title" : "I'm near the solution...","content" : "Poor bacillus!!! Fried in a super basic pH...the media that we used were too basic! so tonight i tried to bring to pH 7 the Groningen's media and tomorrow wi we'll see the truth... meanwhile i prepared the samples for the second sequencing of pspac and pxyl vectors with EFE... i hope that this time there will be something good!!!! ","tags" : "Bacillus"}

@@ Line 1: / Line 1: @@
-{
+{"date" : "2013-07-26","author" : "viola","title" : "<html>I'm near the solution...</html>","content" : "<html>Poor bacillus!!! Fried in a super basic pH...the media that we used were too basic! so tonight i tried to bring to pH 7 the Groningen's media and tomorrow wi we'll see the truth... meanwhile i prepared the samples for the second sequencing of pspac and pxyl vectors with EFE... i hope that this time there will be something good!!!!  </html>","tags" : "Bacillus"}
-	"date" : "2013-07-29",
-	"author" : "emil",
-	"title" : "The incompetent Bacillus saga:the final transformation",
-	"content" : "<html>Finally we have decided to try to transform PXyl+GFP that is an integrative vector for the thr locus.I have digested 1&micro;g PXyl with the enzyme Sca1 following the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion'> standard protocol for the double digestion </a>and exploiting the specific buffer included in the kit from Biolabs.I have previously grown B. subtilis in the <a href='https://2013.igem.org/Team:UNITN-Trento/Protocols#subtilis-transformation'>medium from Groeningen </a> o.n.,this morning I diluted the Bacillus(1:100) in the same medium then when it reached the O.D. of 1.1 I added 1&micro;l of DNA and I let grow for two hours.After that I plated on spectinomicin added LB agar(stock solution 100mg/ml).</html>",
-	"tags" : "B.subtilis-PXyl"
-}