From 2013.igem.org
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| - | <div class ="tbnote">
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| - | <h2>Trojan Horse</h2>
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| - | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Trojan_Horse" target="_blank" class="tbnotelogo THlogo"> ASDF </a>
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| - | <div style="clear: both;"></div>
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| - | <h3>24<sup>th</sup> August</h3>
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| - | <p><b><em>
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| - | </br></em></b></p>
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| - | <p lang="en-US">
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| - | Results: failure: as much GFP+ colonies growing on Kan than on LB
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| - | </p>
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| - | <p lang="en-US">
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| - | <em>PCR from mini Gene sRNA anti Cm</em>
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| - | </p>
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| - | <p lang="en-US">
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| - | 35 H2O
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| - | </p>
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| - | <p lang="en-US">
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| - | 10 Buffer 5x
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| - | </p>
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| - | <p lang="en-US">
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| - | 1 dNTP
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| - | </p>
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| - | <p lang="en-US">
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| - | 2 FW iT009
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| - | </p>
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| - | <p lang="en-US">
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| - | 2 RV iT0010
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| - | </p>
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| - | <p lang="en-US">
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| - | 0.2 minigene-plasmid amp from IDT
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| - | </p>
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| - | <p lang="en-US">
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| - | 0.5 phusion
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| - | </p>
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| - | <p lang="en-US">
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| - | PCR Phusion Gradient around 53 °C
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| - | </p>
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| - | <p lang="en-US">
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| - | Time : 30 secondes
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| - | </p>
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| - | <img src="https://static.igem.org/mediawiki/2013/9/93/ParisB2013TH_IMG-PCR_sRNA_Cm_.jpg" width="200" height="200">
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| - | <img src="https://static.igem.org/mediawiki/2013/7/76/ParisB2013TH_IMG-Cut_sRNA_Cm.jpg" width="200" height="200">
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| - | </div>
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| - | </html>
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Latest revision as of 17:59, 3 October 2013
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