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Team:Heidelberg/Templates/Del week14 AE
From 2013.igem.org
(Difference between revisions)
(→Restriction digest of fragment FS_02 to FS_03; 5.3 kb;(27-07-2013; II) with BglII) |
(→Restriction digest of fragment FS_02 to FS_03; 5.3 kb;(27-07-2013; II) with BglII) |
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| - | ===Restriction digest of fragment FS_02 to FS_03 | + | ===Restriction digest of fragment FS_02 to FS_03 (5.3 kb; 27-07-2013; II) with BglII === |
[[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|100px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|100px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | ||
Latest revision as of 18:38, 3 October 2013
29-07-2013
Restriction digest of fragment FS_02 to FS_03 (5.3 kb; 27-07-2013; II) with BglII
Incubation at 37°C for about 3 h
| what | µL |
|---|---|
| FS_02 to FS_03 (27-07-2013; II) | 15 |
| BglII | 1 |
| Buffer 3.1 | 2 |
| dd H2O | 2 |
| Expected fragment lengths [bp] | 2146, 1862, 1306 |
Results:
- Restriction digest shows the expected product sizes
- indicator for correct amplicon but to be sure, PCR product will be prepared for single read sequencing by GATC
