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Team:Tuebingen/Notebook/Protocols/lb
From 2013.igem.org
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| + | <h3>Reagents</h3> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="80"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">10.0 g</td> | ||
| + | <td>Trypton</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">5.0 g</td> | ||
| + | <td>Yeast extract</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">10.0 g</td> | ||
| + | <td>NaCl</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center"></td> | ||
| + | <td>Antibiotics</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <h3>Procedure</h3> | ||
| + | <p>Inan autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.</p> | ||
| + | |||
| + | |||
| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
Latest revision as of 12:08, 4 October 2013
LB Medium
Reagents
| 10.0 g | Trypton |
| 5.0 g | Yeast extract |
| 10.0 g | NaCl |
| Antibiotics |
Procedure
Inan autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down and add antibiotics if required. Store at 4 °C.
Back to Protocols
