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Team:Tuebingen/Notebook/Protocols/inoue
From 2013.igem.org
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<p>Mix all reagents and fill up to 1 L with Aqua dest. Serilize through filtration (0.45 µm filter) and store at -20°C.</p> | <p>Mix all reagents and fill up to 1 L with Aqua dest. Serilize through filtration (0.45 µm filter) and store at -20°C.</p> | ||
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Latest revision as of 12:09, 4 October 2013
Inoue Buffer
Reagents
| 9.67 g | MnCl2 * 2 H2O |
| 2.2 g | CaCl2 * 2 H2O |
| 18.65 g | KCl |
| 20.0 mL | PIPES (c = 0.5 M ; pH 6.7) |
| to 1 L | Aqua dest. |
Procedure
Mix all reagents and fill up to 1 L with Aqua dest. Serilize through filtration (0.45 µm filter) and store at -20°C.
Back to Protocols
