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Team:Tuebingen/Notebook/Protocols/gelextraction
From 2013.igem.org
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| - | + | Gel Extraction | |
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<h3>Procedure</h3> | <h3>Procedure</h3> | ||
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<li>Elute in 20 µL Genaxxon Buffer 3.2.</li> | <li>Elute in 20 µL Genaxxon Buffer 3.2.</li> | ||
<li>Run eluate twice through the column in order to increase DNA yields.</li> | <li>Run eluate twice through the column in order to increase DNA yields.</li> | ||
| - | <li>Control gelextraction via <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">gelelectrophoresis</a>.</li> | + | <li>Store at -20 °C.</li> |
| + | <li>Control success of gelextraction via <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">gelelectrophoresis</a>.</li> | ||
</ol> | </ol> | ||
| + | <p> </p> | ||
| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
Latest revision as of 12:05, 4 October 2013
Gel Extraction
Procedure
- Follow Genaxxon PCR and Gel extraction Mini Prep Kit Manual
- Elute in 20 µL Genaxxon Buffer 3.2.
- Run eluate twice through the column in order to increase DNA yields.
- Store at -20 °C.
- Control success of gelextraction via gelelectrophoresis.
Back to Protocols
