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Team:Groningen/Labwork/4 July 2013
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| - | <Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) . | + | |
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| + | <h2>Sander and Inne</h2> | ||
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| + | <Br/>For the silk it is decided to try a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) . | ||
<br/>1 2 3 4 5 6 | <br/>1 2 3 4 5 6 | ||
<br/>98 98 85/90 72 72 4 | <br/>98 98 85/90 72 72 4 | ||
<br/>20:00 0:30 0:25 0:27 10:00 forever | <br/>20:00 0:30 0:25 0:27 10:00 forever | ||
| - | Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check). | + | Ran a <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> with the 8 PCR products and the purified promoter from yesterday (as a check). |
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1. = duplo of sample 1 | 1. = duplo of sample 1 | ||
| - | Ran the same gel again for 22 min on 100 volt to get a better picture | + | Ran the same <a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> again for 22 min on 100 volt to get a better picture |
<br />(picture was take about an hour and a half after run finished because of our meeting) | <br />(picture was take about an hour and a half after run finished because of our meeting) | ||
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Latest revision as of 09:53, 30 July 2013
Sander and Inne
For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
| Well | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| promoter | Marker | 1 | 1. | 2 | 2. | 3 | 3. | 4 | 4. |
(picture was take about an hour and a half after run finished because of our meeting)
