Team:Tuebingen/Notebook/Protocols/glycerol-stocks

From 2013.igem.org

(Difference between revisions)
 
Line 27: Line 27:
-
<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
+
<p><a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a></p>
</div>
</div>

Latest revision as of 12:11, 4 October 2013

Return to iGEM Main Page.

Glycerol Stocks

Procedure

  1. Inoculate 5 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
  2. On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.
  3. Add 1 mL of cell-culture and mix.

Back to Protocols


Retrieved from "http://2013.igem.org/Team:Tuebingen/Notebook/Protocols/glycerol-stocks"