Team:Marburg/Notebook:Primer list

From 2013.igem.org

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{{:Team:Marburg/Template:Header}}
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{{:Team:Marburg/Template:ContentTitleNav}}
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Primer list
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Supplementary <html><a href="https://2013.igem.org/Team:Marburg/Notebook:October"><img src="https://static.igem.org/mediawiki/2013/1/13/Mr-igem-previous-arrow.png" alt="Previous" style="float:right;"></a></html>
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{{:Team:Marburg/Template:ContentStartNav}}<html>
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{{:Team:Marburg/Template:ContentStartNav}}<html><div class="suppentry">
 +
<fieldset class="primerlist">
 +
<h2 class="title">
 +
<a name="title">Primer list</a>
 +
</h2>
 +
<br />
<table id="primer_list">
<table id="primer_list">
-
  <col width=130>
+
  <col width=50>
-
  <col width=178>
+
  <col width=192>
-
  <col width=358>
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  <col width=578>
-
  <col width=144>
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  <thead>
-
<tr>
+
   <th height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
-
   <td height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
+
   <th class=xl73 width=207 style='border-left:none;width:155pt'>Name</td>
-
   <td class=xl73 width=207 style='border-left:none;width:155pt'>Name</td>
+
   <th class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
-
   <td class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
+
  </thead>
-
  <td class=xl74 width=229 style='border-left:none;width:172pt'>Comment</td>
+
-
  </tr>
+
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR01</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR01</td>
   <td class=xl65 style='border-top:none;border-left:none'>ble-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>ble-for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 24: Line 26:
   <td class=xl65 style='border-top:none;border-left:none'>ble-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>ble-rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 30: Line 31:
   <td class=xl65 style='border-top:none;border-left:none'>cat-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>cat-for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 36: Line 36:
   <td class=xl65 style='border-top:none;border-left:none'>cat-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>cat-rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 42: Line 41:
   <td class=xl65 style='border-top:none;border-left:none'>P_fcbP-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>P_fcbP-for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 48: Line 46:
   <td class=xl65 style='border-top:none;border-left:none'>P_fcbP-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>P_fcbP-rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 54: Line 51:
   <td class=xl65 style='border-top:none;border-left:none'>P_NR-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>P_NR-for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 60: Line 56:
   <td class=xl65 style='border-top:none;border-left:none'>P_NR-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>P_NR-rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 66: Line 61:
   <td class=xl65 style='border-top:none;border-left:none'>T_fcpA-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>T_fcpA-for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR10</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR10</td>
   <td class=xl65 style='border-top:none;border-left:none'>T_fcpA-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>T_fcpA-rev</td>
-
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAAC
+
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAACTCATCCTGTGCCTT</td>
-
  TCATCCTGTG CCTT</td>
+
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
+
  </tr>
  </tr>
  <tr>
  <tr>
Line 79: Line 71:
   <td class=xl65 style='border-top:none;border-left:none'>T_NR-for</td>
   <td class=xl65 style='border-top:none;border-left:none'>T_NR-for</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 85: Line 76:
   <td class=xl65 style='border-top:none;border-left:none'>T_NR-rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>T_NR-rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 91: Line 81:
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Pvu-For</td>
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Pvu-For</td>
   <td class=xl70 style='border-top:none;border-left:none'>CCAGACCGTTCAGCTAGATATTACG</td>
   <td class=xl70 style='border-top:none;border-left:none'>CCAGACCGTTCAGCTAGATATTACG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 97: Line 86:
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Pvu-Rev</td>
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Pvu-Rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGTAATATCTAGCTGAACGGTCTGG</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGTAATATCTAGCTGAACGGTCTGG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 103: Line 91:
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Eco-For</td>
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Eco-For</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCTCATCCGGAGTTCCGTATGGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCTCATCCGGAGTTCCGTATGGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 109: Line 96:
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Eco-Rev</td>
   <td class=xl66 style='border-top:none;border-left:none'>Cat-bb-Eco-Rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCCATACGGAACTCCGGATGAGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCCATACGGAACTCCGGATGAGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 115: Line 101:
   <td class=xl66 style='border-top:none;border-left:none'>PfcpB-Pvu-For</td>
   <td class=xl66 style='border-top:none;border-left:none'>PfcpB-Pvu-For</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGACGCTCGCTCCAGCTAGGGCTTCCTTAC</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGACGCTCGCTCCAGCTAGGGCTTCCTTAC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 121: Line 106:
   <td class=xl66 style='border-top:none;border-left:none'>PfcpB-Pvu-Rev</td>
   <td class=xl66 style='border-top:none;border-left:none'>PfcpB-Pvu-Rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 127: Line 111:
   <td class=xl66 style='border-top:none;border-left:none'>PNR-Pst-For</td>
   <td class=xl66 style='border-top:none;border-left:none'>PNR-Pst-For</td>
   <td class=xl70 style='border-top:none;border-left:none'>TTCCGGCACCTACAGCGAGGAGTTTTG</td>
   <td class=xl70 style='border-top:none;border-left:none'>TTCCGGCACCTACAGCGAGGAGTTTTG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 133: Line 116:
   <td class=xl66 style='border-top:none;border-left:none'>PNR-Pst-Rev</td>
   <td class=xl66 style='border-top:none;border-left:none'>PNR-Pst-Rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>CAAAACTCCTCGCTGTAGGTGCCGGAA</td>
   <td class=xl70 style='border-top:none;border-left:none'>CAAAACTCCTCGCTGTAGGTGCCGGAA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 139: Line 121:
   <td class=xl66 style='border-top:none;border-left:none'>T_fcpa-Xho-For</td>
   <td class=xl66 style='border-top:none;border-left:none'>T_fcpa-Xho-For</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGATGAGTTTTCTAGAGGCCGGTCTCC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGATGAGTTTTCTAGAGGCCGGTCTCC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 145: Line 126:
   <td class=xl66 style='border-top:none;border-left:none'>T-fcpa-Xho-Rev</td>
   <td class=xl66 style='border-top:none;border-left:none'>T-fcpa-Xho-Rev</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGAGACCGGCCTCTAGAAAACTCATCC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGAGACCGGCCTCTAGAAAACTCATCC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 151: Line 131:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_P_NR_For</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_P_NR_For</td>
   <td class=xl69 style='border-top:none;border-left:none'>CGGGGCTGGCTTAACTAT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CGGGGCTGGCTTAACTAT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 157: Line 136:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_P_NR_Rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_P_NR_Rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTAAATTGAAGCTTGCATGC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTAAATTGAAGCTTGCATGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR25</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR25</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR,
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR and P_fcpB_For</td>
-
  P_fcpB_For</td>
+
   <td class=xl69 style='border-top:none;border-left:none'>ACTTGTGCGAACGGAATT</td>
   <td class=xl69 style='border-top:none;border-left:none'>ACTTGTGCGAACGGAATT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR26</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR26</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR,
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR and P_fcpB_Rev</td>
-
  P_fcpB_Rev</td>
+
   <td class=xl69 style='border-top:none;border-left:none'>CACCGGCGAAGTCGTCCT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CACCGGCGAAGTCGTCCT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 177: Line 151:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_Ble_For</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_Ble_For</td>
   <td class=xl69 style='border-top:none;border-left:none'>CTACGACGCTCGCTCCAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>CTACGACGCTCGCTCCAG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 183: Line 156:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_Ble_Rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_Ble_Rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GGCATCCATGGTGAAGACGA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GGCATCCATGGTGAAGACGA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 189: Line 161:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_fcpA_For</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_fcpA_For</td>
   <td class=xl69 style='border-top:none;border-left:none'>AGATCGGCGAGCAGCCGT</td>
   <td class=xl69 style='border-top:none;border-left:none'>AGATCGGCGAGCAGCCGT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 195: Line 166:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_fcpA_Rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_fcpA_Rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>CCCGCGCGTTGGCCGATT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CCCGCGCGTTGGCCGATT</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 201: Line 171:
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_l_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_l_for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT</td>
-
  <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 207: Line 176:
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_l_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_l_rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC</td>
-
  <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 213: Line 181:
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_h_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_h_for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG</td>
-
  <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 219: Line 186:
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_h_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Cl4mA_h_rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG</td>
-
  <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 225: Line 191:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_for</td>
   <td class=xl69 style='border-top:none;border-left:none'>CGGCGTAATACGACTCACTATA</td>
   <td class=xl69 style='border-top:none;border-left:none'>CGGCGTAATACGACTCACTATA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 231: Line 196:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>ACCTACAACGGTTCCTGATG</td>
   <td class=xl69 style='border-top:none;border-left:none'>ACCTACAACGGTTCCTGATG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 237: Line 201:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_for (VF2)</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_for (VF2)</td>
   <td class=xl70 style='border-top:none;border-left:none'>TGCCACCTGACGTCTAAGAA</td>
   <td class=xl70 style='border-top:none;border-left:none'>TGCCACCTGACGTCTAAGAA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 243: Line 206:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_rev (VR)</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_rev (VR)</td>
   <td class=xl70 style='border-top:none;border-left:none'>ATTACCGCCTTTGAGTGAGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>ATTACCGCCTTTGAGTGAGC</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 249: Line 211:
   <td class=xl65 style='border-top:none;border-left:none'>Seq_cat.int_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_cat.int_rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GCTCATGGAAAACGGTGTAA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GCTCATGGAAAACGGTGTAA</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 255: Line 216:
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_for</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG</td>
-
  <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 261: Line 221:
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 267: Line 226:
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 1 AtpC</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 273: Line 231:
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_rev</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 1 AtpC</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 279: Line 236:
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 2 FCpD</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 285: Line 241:
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_rev</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 2 FCpD</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 291: Line 246:
   <td class=xl65 style='border-top:none;border-left:none'>SP1_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 297: Line 251:
   <td class=xl65 style='border-top:none;border-left:none'>SP1_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_rev</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 303: Line 256:
   <td class=xl65 style='border-top:none;border-left:none'>SP2_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2 CA-VI</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 309: Line 261:
   <td class=xl65 style='border-top:none;border-left:none'>SP2_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_rev</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2 CA-VI</td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 315: Line 266:
   <td class=xl67 style='border-top:none;border-left:none'>RFP_for</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG</td>
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 321: Line 271:
   <td class=xl67 style='border-top:none;border-left:none'>RFP_rev</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_rev</td>
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCAGCACCGGTGGAGTGACG</td>
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCAGCACCGGTGGAGTGACG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
 
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 329: Line 276:
   <td class=xl67 style='border-top:none;border-left:none'>MTS_for</td>
   <td class=xl67 style='border-top:none;border-left:none'>MTS_for</td>
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCGCGCCGCAGCGGGAGGAAG</td>
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCGCGCCGCAGCGGGAGGAAG</td>
-
  <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
 
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
   <td height=22 class=xl75 style='height:16.5pt;border-top:none'>iMR53</td>
   <td height=22 class=xl75 style='height:16.5pt;border-top:none'>iMR53</td>
   <td class=xl67 style='border-top:none;border-left:none'>MTS_rev</td>
   <td class=xl67 style='border-top:none;border-left:none'>MTS_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font13">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTACCTTTCTAGGAAAAGTTGGCGC</td>
-
  class="font10">CTACCTTTCTAGGAAAAGTTGGCGC</font></td>
+
-
  <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 346: Line 286:
   <td class=xl80 style='border-top:none;border-left:none'>RBS_for</td>
   <td class=xl80 style='border-top:none;border-left:none'>RBS_for</td>
   <td class=xl81 style='border-top:none;border-left:none'>AAAGAGGAGAAA</td>
   <td class=xl81 style='border-top:none;border-left:none'>AAAGAGGAGAAA</td>
-
  <td class=xl82 style='border-top:none;border-left:none'>Colonie-PCR PlacI-RBS</td>
 
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
</table>
</table>
 +
</fieldset>
 +
</div>
 +
 +
<div class="suppentry">
 +
<br />
 +
<br />
 +
<fieldset class="internalbb">
 +
<h2 class="title">
 +
<a name="title">Internal BioBricks - Classification</a>
 +
</h2>
 +
<br />
 +
<table class="ibb">
 +
<colgroup>
 +
<col width="30%" />
 +
<col width="5%" />
 +
<col width="30%" />
 +
<col width="5%" />
 +
<col width="30%" />
 +
</colgroup>
 +
<thead>
 +
<th>Internal number</th>
 +
<th>&nbsp;</th>
 +
<th>Part name</th>
 +
<th>&nbsp;</th>
 +
<th>Description</th>
 +
</thead>
 +
<tr>
 +
<td>iBB1</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071001" target="_blank">BBa_K1071001</a></td>
 +
<th>&nbsp;</th>
 +
<td>ble resistance gene</td>
 +
</tr>
 +
<tr>
 +
<td>iBB2</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071002" target="_blank">BBa_K1071002</a></td>
 +
<th>&nbsp;</th>
 +
<td>cat resistance gene</td>
 +
</tr>
 +
<tr>
 +
<td>iBB3</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071003" target="_blank">BBa_K1071003</a></td>
 +
<th>&nbsp;</th>
 +
<td>P<sub>fcpB</sub></td>
 +
</tr>
 +
<tr>
 +
<td>iBB4</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071004" target="_blank">BBa_K1071004</a></td>
 +
<th>&nbsp;</th>
 +
<td>P<sub>NR</sub></td>
 +
</tr>
 +
<tr>
 +
<td>iBB5</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071005" target="_blank">BBa_K1071005</a></td>
 +
<th>&nbsp;</th>
 +
<td>T<sub>fcpA</sub></td>
 +
</tr>
 +
<tr>
 +
<td>iBB6</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071006" target="_blank">BBa_K1071006</a></td>
 +
<th>&nbsp;</th>
 +
<td>T<sub>NR</sub></td>
 +
</tr>
 +
<tr>
 +
<td>iBB7</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071007" target="_blank">BBa_K1071007</a></td>
 +
<th>&nbsp;</th>
 +
<td>antibody light chain</td>
 +
</tr>
 +
<tr>
 +
<td>iBB8</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071008" target="_blank">BBa_K1071008</a></td>
 +
<th>&nbsp;</th>
 +
<td>antibody heavy chain</td>
 +
</tr>
 +
<tr>
 +
<td>iBB9</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071009" target="_blank">BBa_K1071009</a></td>
 +
<th>&nbsp;</th>
 +
<td>eGFP</td>
 +
</tr>
 +
<tr>
 +
<td>iBB10</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071010" target="_blank">BBa_K1071010</a></td>
 +
<th>&nbsp;</th>
 +
<td>SPTP1 (Stroma localization)</td>
 +
</tr>
 +
<tr>
 +
<td>iBB11</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071011" target="_blank">BBa_K1071011</a></td>
 +
<th>&nbsp;</th>
 +
<td>SPTP2 (Stroma localization)</td>
 +
</tr>
 +
<tr>
 +
<td>iBB12</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071012" target="_blank">BBa_K1071012</a></td>
 +
<th>&nbsp;</th>
 +
<td>SP1 (ER localization)</td>
 +
</tr>
 +
<tr>
 +
<td>iBB13</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071013" target="_blank">BBa_K1071013</a></td>
 +
<th>&nbsp;</th>
 +
<td>SP2 (ER localization)</td>
 +
</tr>
 +
<tr>
 +
<td>iBB14-15</td>
 +
<th>&nbsp;</th>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1071014" target="_blank">BBa_K1071014</a></td>
 +
<th>&nbsp;</th>
 +
<td>RFP-MTS</td>
 +
</tr>
 +
</table>
 +
</fieldset>
 +
</div>
 +
 +
<div class="suppentry">
 +
<br />
 +
<br />
 +
<h2 class="title">
 +
<a name="title">Cloning strategies</a>
 +
</h2>
 +
<br />
 +
<fieldset class="cloningstrategies">
 +
<p>Antibody construct (pSB1A3 iBB486476315)</p>
 +
<br />
 +
<img src="https://static.igem.org/mediawiki/2013/3/34/MR_cloning_antibody.png" width="100%" alt="cloningstrategies" />
 +
</fieldset>
 +
<br />
 +
<br />
 +
<fieldset class="cloningstrategies">
 +
<p>Promotor P<sub>NR</sub> strength measurement (pSB1A3 iBB496315)</p>
 +
<br />
 +
<img src="https://static.igem.org/mediawiki/2013/8/87/MR_cloning_PNR.png" width="100%" alt="cloningstrategies" />
 +
</fieldset>
 +
<br />
 +
<br />
 +
<fieldset class="cloningstrategies">
 +
<p>Promotor P<sub>fcpB</sub> strength measurement (pSB1A3 iBB395416)</p>
 +
<br />
 +
<img src="https://static.igem.org/mediawiki/2013/7/7c/MR_cloning_PfcpB.png" width="100%" alt="cloningstrategies" />
 +
</fieldset>
 +
<br />
 +
<br />
 +
<fieldset class="cloningstrategies">
 +
<p>Signal peptide constructs (pSB1A3 iBB4-10/11/12/13-96315)</p>
 +
<br />
 +
<img src="https://static.igem.org/mediawiki/2013/0/08/MR_cloning_signalpeptide.png" width="100%" alt="cloningstrategies" />
 +
</fieldset>
 +
 +
<br />
 +
<br />
 +
</html>
</html>
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Latest revision as of 11:17, 27 October 2013

Supplementary Previous

Primer list


No Name Sequence
iMR01 ble-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC
iMR02 ble-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG
iMR03 cat-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA
iMR04 cat-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC
iMR05 P_fcbP-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC
iMR06 P_fcbP-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA
iMR07 P_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA
iMR08 P_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA
iMR09 T_fcpA-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT
iMR10 T_fcpA-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAACTCATCCTGTGCCTT
iMR11 T_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC
iMR12 T_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG
iMR13 Cat-bb-Pvu-For CCAGACCGTTCAGCTAGATATTACG
iMR14 Cat-bb-Pvu-Rev CGTAATATCTAGCTGAACGGTCTGG
iMR15 Cat-bb-Eco-For GCTCATCCGGAGTTCCGTATGGC
iMR16 Cat-bb-Eco-Rev GCCATACGGAACTCCGGATGAGC
iMR17 PfcpB-Pvu-For CGACGCTCGCTCCAGCTAGGGCTTCCTTAC
iMR18 PfcpB-Pvu-Rev GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG
iMR19 PNR-Pst-For TTCCGGCACCTACAGCGAGGAGTTTTG
iMR20 PNR-Pst-Rev CAAAACTCCTCGCTGTAGGTGCCGGAA
iMR21 T_fcpa-Xho-For GGATGAGTTTTCTAGAGGCCGGTCTCC
iMR22 T-fcpa-Xho-Rev GGAGACCGGCCTCTAGAAAACTCATCC
iMR23 Seq_P_NR_For CGGGGCTGGCTTAACTAT
iMR24 Seq_P_NR_Rev GTAAATTGAAGCTTGCATGC
iMR25 Seq_T_NR and P_fcpB_For ACTTGTGCGAACGGAATT
iMR26 Seq_T_NR and P_fcpB_Rev CACCGGCGAAGTCGTCCT
iMR27 Seq_Ble_For CTACGACGCTCGCTCCAG
iMR28 Seq_Ble_Rev GGCATCCATGGTGAAGACGA
iMR29 Seq_T_fcpA_For AGATCGGCGAGCAGCCGT
iMR30 Seq_T_fcpA_Rev CCCGCGCGTTGGCCGATT
iMR31 Cl4mA_l_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT
iMR32 Cl4mA_l_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC
iMR33 Cl4mA_h_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG
iMR34 Cl4mA_h_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG
iMR35 Seq_pJET12_for CGGCGTAATACGACTCACTATA
iMR36 Seq_pJET12_rev ACCTACAACGGTTCCTGATG
iMR37 Seq_for (VF2) TGCCACCTGACGTCTAAGAA
iMR38 Seq_rev (VR) ATTACCGCCTTTGAGTGAGC
iMR39 Seq_cat.int_rev GCTCATGGAAAACGGTGTAA
iMR40 eGFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG
iMR41 eGFP_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC
iMR42 SPTP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG
iMR43 SPTP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG
iMR44 SPTP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC
iMR45 SPTP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG
iMR46 SP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT
iMR47 SP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG
iMR48 SP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG
iMR49 SP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG
iMR50 RFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG
iMR51 RFP_rev ATATGGATCCAGCACCGGTGGAGTGACG
iMR52 MTS_for ATATGGATCCGCGCCGCAGCGGGAGGAAG
iMR53 MTS_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTACCTTTCTAGGAAAAGTTGGCGC
iMR54 RBS_for AAAGAGGAGAAA


Internal BioBricks - Classification


Internal number   Part name   Description
iBB1   BBa_K1071001   ble resistance gene
iBB2   BBa_K1071002   cat resistance gene
iBB3   BBa_K1071003   PfcpB
iBB4   BBa_K1071004   PNR
iBB5   BBa_K1071005   TfcpA
iBB6   BBa_K1071006   TNR
iBB7   BBa_K1071007   antibody light chain
iBB8   BBa_K1071008   antibody heavy chain
iBB9   BBa_K1071009   eGFP
iBB10   BBa_K1071010   SPTP1 (Stroma localization)
iBB11   BBa_K1071011   SPTP2 (Stroma localization)
iBB12   BBa_K1071012   SP1 (ER localization)
iBB13   BBa_K1071013   SP2 (ER localization)
iBB14-15   BBa_K1071014   RFP-MTS


Cloning strategies


Antibody construct (pSB1A3 iBB486476315)


cloningstrategies


Promotor PNR strength measurement (pSB1A3 iBB496315)


cloningstrategies


Promotor PfcpB strength measurement (pSB1A3 iBB395416)


cloningstrategies


Signal peptide constructs (pSB1A3 iBB4-10/11/12/13-96315)


cloningstrategies


Retrieved from "http://2013.igem.org/Team:Marburg/Notebook:Primer_list"