Team:UCL/Labbook/Week18

From 2013.igem.org

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<p class="major_title">October</p>
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<p class="minor_title">Week 18</p>
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<p class="body_text"><b>Monday 30th September</b></p>
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<p class="body_text">Ligated CMV with MMP9 and transformed into Top10 cells. These were subsequently plated on <a href="https://2013.igem.org/Team:UCL/Project/Protocols">chloramphenicol plates</a>.
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<p class="body_text"><b>Tuesday 1st October</b></p>
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<p class="body_text">Inoculated colonies from plates from September 30th.</p>
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<p class="body_text"><b>Wednesday 2nd October</b></p>
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<p class="body_text"> Performed miniprep and generated <a href="https://2013.igem.org/Team:UCL/Project/Protocols">glycerol stock</a> from incoluation on Oct 1st. </p>
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<p class="body_text"> Proceeded with analytical digest and gel</p>
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<p class="body_text"> Transformed CMV+MMP9 plasmid from Sinobiological into Top10 cells</p>
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<p class="body_text"><b>Thursday 3nd October</b></p>
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<p class="body_text"> Religated CMV with MMP9 and continued with transformation into Top10 cells. </p>
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<p class="body_text"> Inoculated from plates of plasmid from Sinobiological</p>
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<p class="body_text"><b>Friday 4th October</b></p>
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<p class="body_text"> Performed <a href="https://2013.igem.org/Team:UCL/Project/Protocols">miniprep</a>, generation of glycerol stock on inoculation from Oct 3rd</p>
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<p class="body_text"> Continued with analytical digest and <a href="https://2013.igem.org/Team:UCL/Project/Protocols">gel</a></p>
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Latest revision as of 03:51, 5 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18

October

Week 18

Monday 30th September

Ligated CMV with MMP9 and transformed into Top10 cells. These were subsequently plated on chloramphenicol plates.

Tuesday 1st October

Inoculated colonies from plates from September 30th.

Wednesday 2nd October

Performed miniprep and generated glycerol stock from incoluation on Oct 1st.

Proceeded with analytical digest and gel

Transformed CMV+MMP9 plasmid from Sinobiological into Top10 cells

Thursday 3nd October

Religated CMV with MMP9 and continued with transformation into Top10 cells.

Inoculated from plates of plasmid from Sinobiological

Friday 4th October

Performed miniprep, generation of glycerol stock on inoculation from Oct 3rd

Continued with analytical digest and gel

Retrieved from "http://2013.igem.org/Team:UCL/Labbook/Week18"