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- | <html> | + | <html lang="en"> |
| <head> | | <head> |
- | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> | + | <meta charset="UTF-8" /> |
- | <script src="http://cdn.jquerytools.org/1.2.5/full/jquery.tools.min.js?foo"></script> | + | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> |
| + | <meta name="viewport" content="width=device-width, initial-scale=1.0"> |
| + | <style type="text/css"> |
| | | |
- | <link rel="stylesheet" type="text/css" href="http://cs.wellesley.edu/~hcilab/iGEM2012/css/Team.css">
| + | /* hiding the top section*/ |
- | <STYLE type="text/css">
| + | body{position:absolute; top:0px; width:100%; height:100%;} |
- | /* MAIN STYLE DEFINITIONS */ | + | #top-section{ |
- | a{
| + | height:0px; |
- | color:#870203;
| + | border:none; |
- | -webkit-transition-duration:0.3s; | + | width:980px; |
- | -moz-transition-duration:0.3s;
| + | margin:0 auto; |
- | -o-transition-duration:0.3s; | + | padding:0 0 0 0; |
- | }
| + | background-color:transparent; |
| + | overflow:hide; |
| | | |
- | a:hover {
| |
- | color:#3d3f3c;
| |
| } | | } |
| + | #p-logo{display:none;} |
| + | #search-controls{display:none;} |
| + | #top{display:none;} |
| + | .firstHeading{display:none;} |
| + | /* end of hiding the top section*/ |
| | | |
- | a:visited{
| + | /* global setting*/ |
- | color:#870203;
| + | #globalWrapper{position:absolute; top:0px;left:0px; width:100%; padding:0 0 0 0;margin:0 0 0 0;height:100%;font-family: calibri, Arial, Helvetica, sans-serif;} |
- | }
| + | #content{ |
- | | + | background-color:white; |
- | td
| + | border:none; |
- | { | + | width:1340px; |
- | font-family: Helvetica;
| + | /* height:700px;*/ |
- | font-size: 10pt;
| + | marign:0 auto; |
- | vertical-align: top;
| + | padding:0 0 0 0; |
- | text-align: left;
| + | top:-40px; |
- | padding-right: 10px;
| + | |
- | } | + | |
- | | + | |
- | tr
| + | |
- | { | + | |
- | vertical-align: top;
| + | |
- | }
| + | |
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| + | |
- | font-family: Helvetica; | + | |
- | text-transform: uppercase; | + | |
- | color: #3d3f3c; | + | |
- | text-align: left; | + | |
| } | | } |
- | | + | #bodyContent{ |
- |
| + | background-color:transparent; |
- | H4 {
| + | border:none; |
- | font-family: Helvetica; | + | |
- | text-transform: uppercase; | + | |
- | color: #3d3f3c; | + | |
- | text-align: left;
| + | |
| } | | } |
| + | siteSub{ |
| + | display:none; |
| + | border:none; |
| + | } |
| + | contentSub{ |
| + | display:none; |
| + | } |
| + | /* end of global setting*/ |
| | | |
- | /* CONTENT HEADING STYLES - overrides some main.css styling */ | + | /*hiding the footer-box*/ |
| + | #footer-box{display:none;} |
| + | #catlinks{display:none;} |
| + | /*end of hiding the footer book*/ |
| | | |
- | H6 {
| + | /* menu (page, edit ...) */ |
- | font-family:'Caviar Dreams';
| + | #menubar { |
- | font-size:30px;
| + | background-color:transparent; |
- | font-weight:500;
| + | position: relative; |
- | text-align:left;
| + | float:left; |
- | text-transform:uppercase;
| + | white-space: nowrap; |
- | color: #3d3f3c;
| + | top:-6px; |
- | border-bottom:1px solid orangered;
| + | width: 490px; |
- | padding-bottom:10px;
| + | z-index: 5000; |
- | margin:15px 0;
| + | font-family: sans-serif; |
| + | font-size: 55%; |
| + | line-height: 1em; |
| + | z-index:99; |
| } | | } |
| | | |
- | H1, H3 {
| |
- | font-family:'Source Sans Pro';
| |
- | font-weight:600;
| |
- | text-transform:uppercase;
| |
- | }
| |
| | | |
- | H1 {
| + | #aa{ |
- | font-size:20px;
| + | width:100%; |
- | border:none; | + | height:100%; |
- | }
| + | margin:0 0 0 0; |
| + | padding:0 0 0 0; |
| + | float:left; |
| + | position:absolute; |
| | | |
- | img.headshot {
| + | |
- | width: 100px;
| + | |
- | height: auto;
| + | |
- | vertical-align: text-top;
| + | |
| } | | } |
| | | |
- | | + | #b{ |
- | | + | width:100%; |
- | body {
| + | height:100%; |
- | background:#fff; | + | margin:0 0 0 0; |
- | font-family: Helvetica; | + | padding:0 0 0 0; |
| + | float:left; |
| + | display:none; |
| + | |
| } | | } |
- | | + | </style> |
- | content {
| + | <script language="javascript"> |
- | background: transparent;
| + | function changemodel() |
| + | { |
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/2/27/Nju-home-Model.png"; |
| + | document.getElementById("b").style.display="block"; |
| } | | } |
- | | + | function changeproject() |
- | #tracking_nav
| + | |
| { | | { |
- | margin: 0px 0px 0px 950px;
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/c/c6/Nju-home-Project.png"; |
- | position: fixed;
| + | document.getElementById("b").style.display="block"; |
- | color:#bababa;
| + | |
- | border: 1px solid #3d3f3c;
| + | |
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| + | |
- | font-size: 16pt;
| + | |
- | padding: 5px;
| + | |
- | line-height: 120%;
| + | |
| } | | } |
- | | + | function changehuman() |
- | #tracking_nav a { color:#ffffff; text-transform: lowercase;font-size: 16pt;}
| + | |
- | #tracking_nav a:hover {background:#bababa;}
| + | |
- | | + | |
- | #parts_table
| + | |
| { | | { |
- | border: 1px solid #870203;
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/9/94/Human1111.png"; |
- | border-collapse: collapse;
| + | document.getElementById("b").style.display="block"; |
- | width: 70%;
| + | |
- | margin: auto;
| + | |
| } | | } |
- | | + | function changesafety() |
- | #parts_table td
| + | |
| { | | { |
- | text-align: center;
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/f/f0/Nju-home-Safety.png"; |
- | margin: 5px;
| + | document.getElementById("b").style.display="block"; |
- | border: 1px solid #870203;
| + | |
- |
| + | |
| } | | } |
- | | + | function changewetlab() |
- | #parts_table th
| + | |
| { | | { |
- | background-color: #bababa;
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/e/ed/Nju-logo-Wet_lab.png"; |
- | border: 1px solid #870203;
| + | document.getElementById("b").style.display="block"; |
- | color: #ffffff;
| + | |
| } | | } |
- | | + | function changeteam() |
- | .table_part
| + | |
| { | | { |
- | vertical-align: middle;
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/5/55/Nju-home-Team.png"; |
| + | document.getElementById("b").style.display="block"; |
| } | | } |
- | | + | function changeextras() |
- | /* HEADER STYLES: banner, navbar, etc. */ | + | { |
- | #banner { width:300px; display:block; float:left; }
| + | document.getElementById("image").src = "https://static.igem.org/mediawiki/2013/6/68/Nju-home-Extras.png"; |
- | #banner img { width:100%; }
| + | document.getElementById("b").style.display="block"; |
- | | + | |
- | ul#nav {
| + | |
- | width:1200px;
| + | |
- | margin:0 0 0 30px;
| + | |
- | position:relative;
| + | |
| } | | } |
- | | + | function home() |
- | #nav li {
| + | { |
- | color: #bbb;
| + | document.getElementById("b").style.display="none"; |
- | background-color:none;
| + | |
- | margin: 0 50px 0 0;
| + | |
- | float: left;
| + | |
- | position: relative;
| + | |
- | list-style: none;
| + | |
- | text-transform:uppercase;
| + | |
| } | | } |
- | #nav li:last-child { margin:0; }
| + | </script> |
| | | |
- | /* main level link */ | + | </head> |
- | #nav a {
| + | |
- | font-family:'Source Sans Pro', sans-serif;
| + | |
- | font-size:10pt;
| + | |
- | font-weight:500;
| + | |
- | line-height:110%;
| + | |
- | color: inherit;
| + | |
- | text-decoration: none;
| + | |
- | display: block;
| + | |
- | padding: 0 0 0 5px;
| + | |
- | margin: 0;
| + | |
- | }
| + | |
| | | |
- | ul#nav > li > a {
| + | <body style="margin:0px;padding:0px; "> |
- | line-height:12px;
| + | <div style="float:left; position:absolute; z-index:1; display:block; "> |
- | border-left:solid 2px #bbb;
| + | </br></br> |
- | padding:0 0 0 3px;
| + | <a href="https://2013.igem.org"><img src="https://static.igem.org/mediawiki/2013/2/26/NJU-Miniminilogo.png" ></a> |
- | }
| + | </div> |
| + | <div id="aa"> |
| | | |
- | #nav a:hover { | + | <img src="https://static.igem.org/mediawiki/2013/0/0b/NJU_home_Index.png" usemap="#Map"/> |
- | /*background-color: #870203;
| + | <map name="Map" id="Map"> |
- | color: #ffffff;*/
| + | <area shape="rect" coords="400,590,970,679" onmouseover="home();" href="#"onfocus="blur(this);"> |
- | }
| + | <area shape="circle" coords="400,122,82" onmouseover="changemodel();" href="https://2013.igem.org/Team:NJU_China/Modeling" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="236,432,116" onmouseover="changeproject();" href="https://2013.igem.org/Team:NJU_China/Project" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="530,315,110" onmouseover="changehuman();" href="https://2013.igem.org/Team:NJU_China/Human Practice" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="875,173,105" onmouseover="changesafety();" href="https://2013.igem.org/Team:NJU_China/Safety" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="762,388,105" onmouseover="changewetlab();" href="https://2013.igem.org/Team:NJU_China/Wet lab" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="995,362,78" onmouseover="changeteam();" href="https://2013.igem.org/Team:NJU_China/Team" onfocus="blur(this);"/> |
| + | <area shape="circle" coords="1138,490,71" onmouseover="changeextras();" href="https://2013.igem.org/Team:NJU_China/Extras" onfocus="blur(this);"/> |
| + | </map> |
| + | </div> |
| + | <div id ="b"> |
| + | <img id = "image" src="https://static.igem.org/mediawiki/2013/6/68/Nju-home-Extras.png"> |
| + | </div> |
| | | |
- | /* main level link hover */ | + | </body> |
- | #nav .current a, #nav li:hover > a {
| + | |
- | color: #000;
| + | |
- | border-color:orangered;
| + | |
- | }
| + | |
| | | |
- | /* sub levels link hover */
| |
- | #nav ul li:hover a, #nav li:hover li a {
| |
- | border: none;
| |
- | /*background-color: #FA9D1C;*/
| |
- | color:#000;
| |
- | }
| |
- |
| |
- | #nav ul a:hover {
| |
- | color: orangered !important;
| |
- | /*background: #fff url(img/gradient.png) repeat-x 0 -100px !important;
| |
- | text-shadow: 0 1px 1px rgba(0,0,0, .1);*/
| |
- | }
| |
- |
| |
- |
| |
- | /* dropdown */
| |
- | #nav li:hover > ul {
| |
- | /*display: block;*/
| |
- | opacity:1;
| |
- | margin:0;
| |
- | background-color: none;
| |
- | z-index:0;
| |
- | }
| |
- |
| |
- | /* level 2 list */
| |
- | #nav ul {
| |
- | /*display: none;*/
| |
- | opacity:0;
| |
- | margin: 20px 0 0 0;
| |
- | padding: 7px 0 0 0;
| |
- | width: 205px;
| |
- | position: absolute;
| |
- | left: 0;
| |
- | z-index:-1;
| |
- | -webkit-transition-duration:0.5s;
| |
- | -moz-transition-duration:0.5s;
| |
- | -o-transition-duration:0.5s;
| |
- | }
| |
- | #nav ul li {
| |
- | float: none;
| |
- | margin: 0;
| |
- | padding: 0;
| |
- | }
| |
- |
| |
- | #nav ul a {
| |
- | font-weight: normal;
| |
- | /*text-shadow: 0 1px 0 #fff;*/
| |
- | }
| |
- |
| |
- | /* clearfix */
| |
- | #nav:after {
| |
- | content: ".";
| |
- | display: block;
| |
- | clear: both;
| |
- | visibility: hidden;
| |
- | line-height: 0;
| |
- | height: 0;
| |
- | }
| |
- | #nav {
| |
- | display: inline-block;
| |
- | }
| |
- | html[xmlns] #nav {
| |
- | display: block;
| |
- | }
| |
- |
| |
- | * html #nav {
| |
- | height: 1%;
| |
- | }
| |
- |
| |
- | /* pinterest like photo grid for social page*/
| |
- |
| |
- | /*
| |
- | body {
| |
- | background: url(http://subtlepatterns.com/patterns/scribble_light.png) ;
| |
- | }
| |
- | */
| |
- |
| |
- | #wrapper {
| |
- | width: 90%;
| |
- | max-width: 1100px;
| |
- | min-width: 800px;
| |
- | margin: 50px auto;
| |
- | }
| |
- |
| |
- | #columns {
| |
- | -webkit-column-count: 3;
| |
- | -webkit-column-gap: 10px;
| |
- | -webkit-column-fill: auto;
| |
- | -moz-column-count: 3;
| |
- | -moz-column-gap: 10px;
| |
- | -moz-column-fill: auto;
| |
- | column-count: 3;
| |
- | column-gap: 15px;
| |
- | column-fill: auto;
| |
- | }
| |
- |
| |
- | .pin {
| |
- | display: inline-block;
| |
- | background: #FEFEFE;
| |
- | border: 2px solid #FAFAFA;
| |
- | box-shadow: 0 1px 2px rgba(34, 25, 25, 0.4);
| |
- | margin: 0 2px 15px;
| |
- | -webkit-column-break-inside: avoid;
| |
- | -moz-column-break-inside: avoid;
| |
- | column-break-inside: avoid;
| |
- | padding: 15px;
| |
- | padding-bottom: 5px;
| |
- | background: -webkit-linear-gradient(45deg, #FFF, #F9F9F9);
| |
- | opacity: 1;
| |
- |
| |
- | -webkit-transition: all .2s ease;
| |
- | -moz-transition: all .2s ease;
| |
- | -o-transition: all .2s ease;
| |
- | transition: all .2s ease;
| |
- | }
| |
- |
| |
- | .pin img {
| |
- | width: 100%;
| |
- | border-bottom: 1px solid #ccc;
| |
- | padding-bottom: 15px;
| |
- | margin-bottom: 5px;
| |
- | }
| |
- |
| |
- | .pin p {
| |
- | font: 12px/18px Arial, sans-serif;
| |
- | color: #333;
| |
- | margin: 0;
| |
- | }
| |
- |
| |
- | @media (min-width: 960px) {
| |
- | #columns {
| |
- | -webkit-column-count: 4;
| |
- | -moz-column-count: 4;
| |
- | column-count: 4;
| |
- | }
| |
- | }
| |
- |
| |
- | @media (min-width: 1100px) {
| |
- | #columns {
| |
- | -webkit-column-count: 5;
| |
- | -moz-column-count: 5;
| |
- | column-count: 5;
| |
- | }
| |
- | }
| |
- |
| |
- | #columns:hover .pin:not(:hover) {
| |
- | opacity: 0.4;
| |
- | }
| |
- | </STYLE>
| |
- |
| |
- | <link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:400,600' rel='stylesheet' type='text/css'>
| |
- |
| |
- | <!--Start NavBar-->
| |
- | <ul id="nav">
| |
- | <li><a href="https://2013.igem.org/Team:NJU_China">Home</a></li>
| |
- |
| |
- | <li><a href="https://2013.igem.org/Team:NJU_China/Team">Team</a></li>
| |
- |
| |
- | <li><a href="https://2013.igem.org/Team:NJU_China/Project">Project</a>
| |
- | <ul>
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- | <li><a href="http://https://2013.igem.org/Team:NJU_China/Project#Overall_project">Project Overview</a></li>
| |
- | <li><a https://2013.igem.org/Team:NJU_China/Project#Project_Details">Project Details</a></li>
| |
- | <li><a https://2013.igem.org/Team:NJU_China/Project#Part_2">Part2</a></li>
| |
- | <li><a https://2013.igem.org/Team:NJU_China/Project#The_Experiments">The_Experiments</a></li>
| |
- | <li><a https://2013.igem.org/Team:NJU_China/Project#Part_3">Part3</a></li>
| |
- | <li><a https://2013.igem.org/Team:NJU_China/Project#Results">Results</a></li>
| |
- | </ul>
| |
- | </li>
| |
- |
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- | <li><a href="https://2013.igem.org/Team:NJU_China/Parts">Parts</a></li>
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- |
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- | <li><a href="https://2013.igem.org/Team:NJU_China/Modeling">Modeling</a></li>
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- |
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- | <li><a href="https://2013.igem.org/Team:NJU_China/Notebook">Notebook</a></li>
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- |
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- | <li><a href="https://2013.igem.org/Team:NJU_China/Safety">Safety</a></li>
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- |
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- | <li><a href="https://2013.igem.org/Team:NJU_China/Attributions">Attributions</a></li>
| |
- |
| |
- | </ul>
| |
- | <!--End NavBar-->
| |
- | </body>
| |
| </html> | | </html> |
- |
| |
- |
| |
- |
| |
- | {|align="justify"
| |
- |
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- |
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- | [[Image:NJU China TeamLogo.png|1000px]]
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- |
| |
- | |'''Biomissile'''
| |
- | Site-specific drug delivery has always been a challenge in gene therapy. It is urgently required to develop a new system to overcome the off-target effect, low efficiency and high toxicity of the currently available approaches. Recently, small interfering RNA (siRNA) is emerging as a promising therapeutic drug against a wide array of diseases. Current techniques for siRNA transfer use viruses or synthetic agents as delivery vehicles. However, these approaches are toxic and low-efficient, and more importantly, can not deliver siRNA to specific tissues and organs.
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- |
| |
- | {|align="justify"
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- |
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- | |To better deliver the siRNA and other drugs, we are trying to construct a novel system which employs MVs to encapsulate and protect siRNAs for delivery to target cells. Microvesicles (MVs) are lipid bilayer vesicles which
| |
- | {|align="justify"
| |
- | |are naturally secreted by almost all cell types, playing crucial roles in intercellular transport of bioactive molecules, including siRNAs. Given the intrinsic ability to traverse biological barriers and to naturally transport functional siRNAs between cells, MVs potentially represent a novel and exciting drug carrier for therapeutic uses. Thus, MVs derived from cells engineered to express siRNAs may be capable of delivering siRNAs to local cellular environment.
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- |
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- | To achieve site-specific siRNA delivery, we will express targeting protein onto the surface of MVs and the modified MVs will, in theory, have the ability to target specific tissues and organs. By transfecting the producer cells with siRNA plasmids and collect MVs, we will fill the MVs with therapeutic siRNAs.
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- |
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- | Our modified MVs are just like the “biomissile”, which can be delivered to specific cells and destroy target mRNAs. Our project will open up avenues for therapeutic applications of MVs as biomissile.
| |
- | |[[Image:NJU China Team.JPG|520px|right]]
| |
- | |-|
| |
- | |align="center"|[[Team:NJU_China | Team NJU_China]]
| |
- | |}
| |
- |
| |
- | <!--- The Mission, Experiments
| |
- |
| |
- | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| |
- | !align="center"|[[Team:NJU_China|Home]]
| |
- | !align="center"|[[Team:NJU_China/Team|Team]]
| |
- | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=NJU_China Official Team Profile]
| |
- | !align="center"|[[Team:NJU_China/Project|Project]]
| |
- | !align="center"|[[Team:NJU_China/Parts|Parts Submitted to the Registry]]
| |
- | !align="center"|[[Team:NJU_China/Modeling|Modeling]]
| |
- | !align="center"|[[Team:NJU_China/Notebook|Notebook]]
| |
- | !align="center"|[[Team:NJU_China/Safety|Safety]]
| |
- | !align="center"|[[Team:NJU_China/Attributions|Attributions]]
| |
- | |}--->
| |