"
Team:UCL/Notebook/August
From 2013.igem.org
(Created page with "<html> <head> <script type="text/javascript" src="https://2013.igem.org/Team:UCL/static/head.js?action=raw&ctype=text/javascript"> </script> <link rel="stylesheet" type="text...") |
|||
| (One intermediate revision not shown) | |||
| Line 61: | Line 61: | ||
<p class="body_text"> | <p class="body_text"> | ||
Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter. | Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter. | ||
| - | The team met to discuss fundraising ideas | + | The team met to discuss fundraising ideas such as Kickstarter. Starting idea: brain-with-plaques-for-sale. |
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| - | + | The concept of a Memory Lane, where people could upload their memories onto a page within the wiki, was discussed. | |
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| Line 75: | Line 75: | ||
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| - | + | Team abstracts were written and uploaded onto the wiki. | |
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| Line 110: | Line 110: | ||
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| - | Darren | + | Dr Darren Nesbeth visted the lab on numerous occasions to check project progress. |
The requested batch of biobricks arrived as glycerol stocks. | The requested batch of biobricks arrived as glycerol stocks. | ||
</p> | </p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| - | The team discussed | + | The team further discussed crowdfunding via Kickstarter. Beautiful pieces of art are to be generated by FYi for the Memory Palace. |
</p> | </p> | ||
<p class="minor_title">12th August</p> | <p class="minor_title">12th August</p> | ||
<p class="body_text"> | <p class="body_text"> | ||
| - | + | A strategy meeting was held in the lab with Dr Darren Nesbeth. | |
</p> | </p> | ||
| + | <p class="body_text"> | ||
FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts. | FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts. | ||
| + | The team also debated on the wiki design and a consensus was reached regarding the site map, default banner and logo. | ||
</p> | </p> | ||
| - | + | <p class="body_text"> | |
| - | < | + | |
In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up. | In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The website came to life today! | The website came to life today! | ||
</p> | </p> | ||
| Line 135: | Line 137: | ||
Alex and Oran came up with the idea of a Creative writing competition. | Alex and Oran came up with the idea of a Creative writing competition. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs. | FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs. | ||
</p> | </p> | ||
| Line 143: | Line 146: | ||
The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection. | The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering. | Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering. | ||
</p> | </p> | ||
| Line 152: | Line 156: | ||
<p class="minor_title">16th August</p> | <p class="minor_title">16th August</p> | ||
| - | + | <p class="body_text"> | |
| - | + | Alex finished the essay on Neuroethics on which he has dedicated around 2 weeks of research. | |
| - | + | </p> | |
| - | <p class=" | + | |
| - | + | ||
| - | + | ||
<p class="minor_title">19th August</p> | <p class="minor_title">19th August</p> | ||
| Line 163: | Line 164: | ||
Alex advertised the writing competition on prizemagic.co.uk. | Alex advertised the writing competition on prizemagic.co.uk. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze. | Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze. | ||
</p> | </p> | ||
| Line 170: | Line 172: | ||
The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback. | The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The members’ Profiles are ready to be uploaded on wiki! | The members’ Profiles are ready to be uploaded on wiki! | ||
</p> | </p> | ||
| Line 177: | Line 180: | ||
The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us. | The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered. | The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered. | ||
</p> | </p> | ||
| Line 184: | Line 188: | ||
The first Creative Competition Entry! Yey! Thank you! | The first Creative Competition Entry! Yey! Thank you! | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin. | The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin. | ||
</p> | </p> | ||
| - | The | + | <p class="body_text"> |
| + | The Zeocin kill curve was derived, a concentration of 150 ug/ml was used. | ||
</p> | </p> | ||
| Line 193: | Line 199: | ||
The main poster for the front page was finalised. Well done FYi! | The main poster for the front page was finalised. Well done FYi! | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
New submissions for the Creative writing! | New submissions for the Creative writing! | ||
Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling! | Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling! | ||
| Line 202: | Line 209: | ||
The lab was closed today hence we all focused on the wiki content. | The lab was closed today hence we all focused on the wiki content. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The front page poster background - wasteland was completed. | The front page poster background - wasteland was completed. | ||
</p> | </p> | ||
| Line 207: | Line 215: | ||
<p class="minor_title">27th August</p> | <p class="minor_title">27th August</p> | ||
| + | <p class="body_text"> | ||
| + | Weiling emailed Geneious and Eppendorf with regards to Sponsorship. | ||
| + | </p> | ||
<p class="minor_title">28th August</p> | <p class="minor_title">28th August</p> | ||
| Line 212: | Line 223: | ||
The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th. | The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
We met Darren at 4 pm in the lab to discuss about the biobrick processing. | We met Darren at 4 pm in the lab to discuss about the biobrick processing. | ||
</p> | </p> | ||
| Line 219: | Line 231: | ||
We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences. | We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences. | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible. | Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible. | ||
</p> | </p> | ||
| Line 226: | Line 239: | ||
We uploaded the first samples of memories on the Memory Lane page. | We uploaded the first samples of memories on the Memory Lane page. | ||
</p> | </p> | ||
| + | |||
</div> | </div> | ||
Latest revision as of 03:15, 5 October 2013
August
1st August
Bacterial lab had good results today in the preparation of a new stock of competent cells. In the evening we celebrated the success of the speed debate.
2nd August
Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter. The team met to discuss fundraising ideas such as Kickstarter. Starting idea: brain-with-plaques-for-sale.
The concept of a Memory Lane, where people could upload their memories onto a page within the wiki, was discussed.
Alex suggested a collaboration with Westminster iGEM team regarding the speed debate idea.
5th August
Snapshots of the team members were taken!
Team abstracts were written and uploaded onto the wiki.
Alex contacted the Imperial iGEM team regarding an eventual collaboration.
6th August
Rob invited the team at 12 noon in the Anatomy Hub to discuss about the wiki design in order to make sure that all the ideas about this matter are taken into account.
7th August
Barbeque evening, venue Wilkins Roof Garden!
Prof. Eli Keshavarz-Moore was our guest and at 3 pm we also had the chance to present our project. (venue: Malet Place Engineering LT 1.03)
8th August
The team discussed about the work on zeocin,pA-f1-Zec biobrick, which will indeed be an improvement of BBa_J176124 because:
i) it gives most of the functionality of BBa_J176124 but is compatible with standard assembly
ii) it allows people to simply insert a PROMOTER-ORF fragment upstream of a pA to give an expression cassette for the ORF of interest, and a ZEC to select stable transfectants.
9th August
Project description is up on Wiki!
Dr Darren Nesbeth visted the lab on numerous occasions to check project progress. The requested batch of biobricks arrived as glycerol stocks.
The team further discussed crowdfunding via Kickstarter. Beautiful pieces of art are to be generated by FYi for the Memory Palace.
12th August
A strategy meeting was held in the lab with Dr Darren Nesbeth.
FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts. The team also debated on the wiki design and a consensus was reached regarding the site map, default banner and logo.
In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up.
The website came to life today!
13th August
Alex and Oran came up with the idea of a Creative writing competition.
FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs.
14th August
The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection.
Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering.
15th August
Continued intensively planning and brainstorming for the design of our wiki, especially on the front page design.
16th August
Alex finished the essay on Neuroethics on which he has dedicated around 2 weeks of research.
19th August
Alex advertised the writing competition on prizemagic.co.uk.
Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze.
20th August
The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback.
The members’ Profiles are ready to be uploaded on wiki!
21th August
The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us.
The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered.
22th August
The first Creative Competition Entry! Yey! Thank you!
The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin.
The Zeocin kill curve was derived, a concentration of 150 ug/ml was used.
23th August
The main poster for the front page was finalised. Well done FYi!
New submissions for the Creative writing! Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling!
26th August
The lab was closed today hence we all focused on the wiki content.
The front page poster background - wasteland was completed.
27th August
Weiling emailed Geneious and Eppendorf with regards to Sponsorship.
28th August
The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th.
We met Darren at 4 pm in the lab to discuss about the biobrick processing.
29th August
We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences.
Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible.
30th August
We uploaded the first samples of memories on the Memory Lane page.
