Exeter/7 July 2013

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'''Making standard liquid cultures of our transformed cells'''
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In each 10ml Falcon, we need...
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==Making standard liquid cultures of our transformed cells==
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- 5ml LB broth
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In each 10 ml Falcon, we need:
-
- 5ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
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- 5 ml LB broth
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We used a 50ml conical flask of LB broth which had been autoclaved and added 50ul of chloramphenicol to make a "stock" to be pipetted into the Falcons.
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- 5 &micro;l antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
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Stab the chosen colony with a pipette tip and eject into the Falcon tube.  
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We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 &micro;l of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.
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The tubes are incubated overnight in a spinning 37oC incubator.
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We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.
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The tubes were incubated overnight in a shaking incubator at 37 &deg;C.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Latest revision as of 20:47, 1 October 2013

Exeter iGEM 2013 · Paint by Coli

Making standard liquid cultures of our transformed cells

In each 10 ml Falcon, we need:

- 5 ml LB broth

- 5 µl antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)

We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 µl of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.

We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.

The tubes were incubated overnight in a shaking incubator at 37 °C.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/7_July_2013"