"
Team:METU Turkey/coumeric-acid.html
From 2013.igem.org
Ilkem.kumru (Talk | contribs) |
Linoinsenger (Talk | contribs) |
||
| (10 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
<html> | <html> | ||
| + | <style> | ||
| + | body | ||
| + | { | ||
| + | background-color:#F3F781; | ||
| + | } | ||
| + | </style> | ||
<head> | <head> | ||
| Line 67: | Line 73: | ||
<div class="clearfix colelem" id="pu20661"><!-- group --> | <div class="clearfix colelem" id="pu20661"><!-- group --> | ||
<div class="grpelem" id="u20661"><!-- image --> | <div class="grpelem" id="u20661"><!-- image --> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
<img class="block" id="u20661_img" src="http://ghucuk.netii.net/8.png" alt="" width="755" height="566"/> | <img class="block" id="u20661_img" src="http://ghucuk.netii.net/8.png" alt="" width="755" height="566"/> | ||
| + | <p> </p> | ||
</div> | </div> | ||
<ul class="MenuBar gradient clearfix grpelem" id="menuu7920"><!-- row --> | <ul class="MenuBar gradient clearfix grpelem" id="menuu7920"><!-- row --> | ||
| Line 143: | Line 152: | ||
<p>• <span id="u20660-2">P- coumaric Acid Production:</span></p> | <p>• <span id="u20660-2">P- coumaric Acid Production:</span></p> | ||
<p>The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.</p> | <p>The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.</p> | ||
| - | + | ||
| - | <p> In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.</p> | + | |
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> In our circuit, Sam8 gene is transcribed with <i> B. subtilis</i> specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.</p> | ||
<p id="u20660-22"> </p> | <p id="u20660-22"> </p> | ||
| Line 154: | Line 172: | ||
</div> | </div> | ||
<div class="clearfix grpelem" id="u20667-4"><!-- content --> | <div class="clearfix grpelem" id="u20667-4"><!-- content --> | ||
| + | <p> </p> | ||
<p>Mathematical model:</p> | <p>Mathematical model:</p> | ||
</div> | </div> | ||
Latest revision as of 03:26, 5 October 2013
• P- coumaric Acid Production:
The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.
In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.
Mathematical model:
Differential equations:
• In our model, we calculated the
[1]
Simulation:
• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s , 12E9 molecule of p-coumaric acid is produced according to our model.
