"
Team:Hong Kong CUHK/overview
From 2013.igem.org
(Difference between revisions)
Shengry0716 (Talk | contribs) |
|||
| (3 intermediate revisions not shown) | |||
| Line 38: | Line 38: | ||
<style type="text/css"> | <style type="text/css"> | ||
<!-- | <!-- | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
body{background-color:#660099} | body{background-color:#660099} | ||
#globalWrapper { | #globalWrapper { | ||
| Line 54: | Line 49: | ||
#content | #content | ||
{width: 1000px;border:none;background-color:#660099;padding:0px;margin-top:0px;line-height: 1.5em;color: black;} | {width: 1000px;border:none;background-color:#660099;padding:0px;margin-top:0px;line-height: 1.5em;color: black;} | ||
| - | #footnote { | + | #footnote {background-color:#FFFF93; |
position:absolute; | position:absolute; | ||
left:0; | left:0; | ||
| Line 77: | Line 72: | ||
#containerX2{ | #containerX2{ | ||
position:relative; | position:relative; | ||
| - | width: | + | width:1100px; |
| - | height: | + | height:600px; |
z-index:1; | z-index:1; | ||
background-color: #FFF; | background-color: #FFF; | ||
} | } | ||
| + | #containerX4{ | ||
| + | position:relative; | ||
| + | width:1025px; | ||
| + | height:600px; | ||
| + | left:15px; | ||
| + | z-index:1; | ||
| + | background-color: #FFF; | ||
| + | } | ||
| + | |||
#partable { | #partable { | ||
top:75px; | top:75px; | ||
| Line 104: | Line 108: | ||
<div id="buttons"> | <div id="buttons"> | ||
| - | <div class="menubar" id="homeUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/e/ee/Bpurple_home.png" width=" | + | <div class="menubar" id="homeUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK"><img src="https://static.igem.org/mediawiki/2013/e/ee/Bpurple_home.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="teamUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/team"><img src="https://static.igem.org/mediawiki/2013/d/d8/Bpurple_team.png" width=" | + | <div class="menubar" id="teamUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/team"><img src="https://static.igem.org/mediawiki/2013/d/d8/Bpurple_team.png" width="110" height="71" /></a></div> |
| - | <div class="menubar" id="projectUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/project"><img src="https://static.igem.org/mediawiki/2013/2/2d/Bpurple_project.png" width=" | + | <div class="menubar" id="projectUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/project"><img src="https://static.igem.org/mediawiki/2013/2/2d/Bpurple_project.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="biobrickUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/biobrick"><img src="https://static.igem.org/mediawiki/2013/b/b6/Bpurple_biobrick.png" width=" | + | <div class="menubar" id="biobrickUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/biobrick"><img src="https://static.igem.org/mediawiki/2013/b/b6/Bpurple_biobrick.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="hpUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/humanpractice"><img src="https://static.igem.org/mediawiki/2013/9/95/Bpurple_human_practice.png" width=" | + | <div class="menubar" id="hpUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/humanpractice"><img src="https://static.igem.org/mediawiki/2013/9/95/Bpurple_human_practice.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="modelUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/modelling"><img src="https://static.igem.org/mediawiki/2013/3/3f/Bpurple_modelling.png" width=" | + | <div class="menubar" id="modelUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/modelling"><img src="https://static.igem.org/mediawiki/2013/3/3f/Bpurple_modelling.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="safetyUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/safety"><img src="https://static.igem.org/mediawiki/2013/1/1e/Bpurple_safety.png" width=" | + | <div class="menubar" id="safetyUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/safety"><img src="https://static.igem.org/mediawiki/2013/1/1e/Bpurple_safety.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="docUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/documentation"><img src="https://static.igem.org/mediawiki/2013/1/12/Bpurple_documentation.png" width=" | + | <div class="menubar" id="docUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/documentation"><img src="https://static.igem.org/mediawiki/2013/1/12/Bpurple_documentation.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="galleryUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/gallery"><img src="https://static.igem.org/mediawiki/2013/e/ed/Bpurple_gallery.png" width=" | + | <div class="menubar" id="galleryUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/gallery"><img src="https://static.igem.org/mediawiki/2013/e/ed/Bpurple_gallery.png" width="110" height="71"/></a></div> |
| - | <div class="menubar" id="ackUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/acknowledgement"><img src="https://static.igem.org/mediawiki/2013/4/4b/Bpurple_ack.png" width=" | + | <div class="menubar" id="ackUP"><a href="https://2013.igem.org/Team:Hong_Kong_CUHK/acknowledgement"><img src="https://static.igem.org/mediawiki/2013/4/4b/Bpurple_ack.png" width="110" height="71"/></a></div> |
</div> | </div> | ||
| Line 126: | Line 130: | ||
<div id="middle"> | <div id="middle"> | ||
<div id="containerX2" style="margin: 0px auto;"> | <div id="containerX2" style="margin: 0px auto;"> | ||
| + | <div id="containerX4" style="margin: 0px auto;"> | ||
<div id="heading" style="margin: 0px auto;"> | <div id="heading" style="margin: 0px auto;"> | ||
<h2>Overview</h2> | <h2>Overview</h2> | ||
| Line 138: | Line 143: | ||
</p3> | </p3> | ||
</div> | </div> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 150: | Line 156: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 14:11, 28 October 2013
Overview
The goal of our project is to develop a method using E.coli to degrade polycyclic aromatic hydrocarbons (PAHs) into other less toxic chemicals. PAHs are notorious for their damage on the environment and human health. These compounds can be found anywhere, from second-hand smoke to cooking fume. The wide spread and toxicity of PAHs indeed arouse our concern and interest to tackle the issue.
We proposed the PAHs degradation system, which contains codon-optimized laccase from Bacillus sp. HR03 and catechol 1,2-dioxygenase from Pseudomonas putida KT2440 for Escherichia coli. Laccase can first degrade PAHs into quinones, and followed by catechol 1,2-dioxygenase, quinones would further be degraded into some simple carboxylic acid. The final product is a much less harmful compound to the human body. For the regulation of this PAHs degradation system, we added quinone sensing and response repressor (QsrR) from Staphylococcus aureus, as quinones are generated at the beginning of the metabolism.
Another great highlight of our project is the Voltage Switch (VS), which can help to accelerate the PAHs degradation. We designed a novel transmembrane protein called Voltage Switch (VS), which is a fusion protein utilizing the voltage sensing domain from potassium ion channels. Triggered by change in potential across the cell membrane, VS can separate or bring targeting enzymes into proximity; thus allowing an instant control of the enzymatic reaction.
