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Team:Penn/MaGellinToolbox
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| - | <b>Constructing a Toolbox</b>Our goal was to create a toolbox for developing customized site-specific methylases. Like any good toolbox, it includes three components: the tool, the assay, and the automation. | + | For a detailed, graphical explanation of the MaGellin work flow, please download the <a href="https://static.igem.org/mediawiki/2013/e/e5/Spec_Sheet.pdf">MaGellin Workflow Specifications Sheet</a>, which includes all of the steps in the MaGellin workflow. |
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| + | <h4><b>Constructing a Toolbox</b></h4>Our goal was to create a toolbox for developing customized site-specific methylases. Like any good toolbox, it includes three components: the tool, the assay, and the automation. | ||
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<li>The Tool: a fusion between a DNA binding domain and a methylase enzyme</li> | <li>The Tool: a fusion between a DNA binding domain and a methylase enzyme</li> | ||
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| + | <center><a href="https://2013.igem.org/Team:Penn/MaGellinMotivation">←Previous</a> <a href="https://2013.igem.org/Team:Penn/AssayOverview">Next→</a></center> | ||
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Latest revision as of 20:11, 28 October 2013
Toolbox
Constructing a Toolbox
Our goal was to create a toolbox for developing customized site-specific methylases. Like any good toolbox, it includes three components: the tool, the assay, and the automation.- The Tool: a fusion between a DNA binding domain and a methylase enzyme
- The Assay: a digestion based assay, called MaGellin, to measure both methylase activity and sequence specificity
- The Automation: a unique software package that predicts experimental outcomes and analyzes gel electrophoresis images to measure methylase functionality
- Robust – includes functional fusion proteins for comparison
- Standardized – all inclusive in one-plasmid with simple multiple cloning sites, standard bisulfite sequencing primers, and a digestion based methylation assay built in
- Easy to Use – we programmed a software package to predict experimental outcomes and automate gel electrophoresis analysis
- Inexpensive – methylase activity and specificity can be screened by simply digesting and running a gel
- Noiseless – the bacterial chassis has no background CpG methylation
- Open Source – we BioBrick’ed the full plasmid and the methylases
