Team:EPF Lausanne/Calendar/28 October 2013

From 2013.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
<font size = "4"> Cell Surface Display</font>
 +
 +
<BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR>
 +
 +
Repeating the microscopy
 +
<br>- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
 +
<br>- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-YFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-YFP antibody (revealed with avidin dylight (red)).
 +
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.
 +
 +
<br><br><br>
 +
<font size = "4"> Nanoparticles</font> <BR>
<font size = "4"> Nanoparticles</font> <BR>

Latest revision as of 23:43, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display


CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

Repeating the microscopy
- Samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
- Different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-YFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-YFP antibody (revealed with avidin dylight (red)).
- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.




Nanoparticles

Microfluidic
- Repeat microfluidics experiment for nanoparticles digestion with MMP2

Wiki Links

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/28_October_2013"